GapMind for catabolism of small carbon sources

 

thymidine catabolism in Klebsiella michiganensis M5al

Best path

nupG, deoA, deoB, deoC, adh, ackA, pta

Also see fitness data for the top candidates

Rules

Overview: Thymidine degradation in GapMind is based on thymidine phoshorylase (EC 2.4.2.4), which yields 2-deoxyribose-1-phosphate and thymine. The catabolism of thymine is not represented, as it may be excreted.

12 steps (11 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
nupG thymidine permease NupG/XapB BWI76_RS24170 BWI76_RS20600
deoA thymidine phosphorylase DeoA BWI76_RS04135 BWI76_RS01295
deoB phosphopentomutase BWI76_RS04140 BWI76_RS25130
deoC deoxyribose-5-phosphate aldolase BWI76_RS04130 BWI76_RS23665
adh acetaldehyde dehydrogenase (not acylating) BWI76_RS21985 BWI76_RS15205
ackA acetate kinase BWI76_RS20235 BWI76_RS23200
pta phosphate acetyltransferase BWI76_RS20240 BWI76_RS20805
Alternative steps:
acs acetyl-CoA synthetase, AMP-forming BWI76_RS02095 BWI76_RS17800
ald-dh-CoA acetaldehyde dehydrogenase, acylating BWI76_RS17250 BWI76_RS20790
nupC thymidine permease NupC BWI76_RS20540 BWI76_RS10810
Slc28a3 thymidine:Na+ symporter SLC28A3 BWI76_RS04125 BWI76_RS19710
Slc29a1 thymidine transporter Slc29a1

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Aug 02 2021. The underlying query database was built on Aug 02 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory