L-valine catabolism in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

L-valine catabolism in Klebsiella michiganensis M5al

Best path

livF, livG, livJ, livH, livM, ofo, acdH, ech, bch, mmsB, mmsA, prpC, acnD, prpF, acn, prpB

Also see fitness data for the top candidates

Rules

Overview: Valine degradation in GapMind is based on MetaCyc pathway L-valine degradation I (link). The other pathways do not produce any fixed carbon and are not included.

all: valine-transport, BKD, acdH, ech, bch, mmsB, mmsA and propionyl-CoA-degradation

Comment: An aminotransferase (not represented) forms 3-methyl-2-oxobutanoate, the decarboxylating alpha-ketoacid dehydrogenase (BKD) forms isobutanoyl-CoA, dehydrogenase acdH forms methylacrylyl-CoA (2-methylprop-2-enoyl-CoA), the hydratase ech forms (S)-3-hydroxy-isobutaonoyl-CoA, a hydrolase forms (S)-3-hydroxy-isobutanoate, a dehydrogenase forms (S)-methylmalonate semialdehyde (2-methyl-3-oxopropanoate), and a decarboxylating dehydrogenase forms propionyl-CoA.

BKD:

bkdA, bkdB, bkdC and lpd
or vorA, vorB and vorC
or ofoA and ofoB
or ofo
Comment: These decarboxylating dehydrogenases act on 4-methyl-2-oxopentanoate, 3-methyl-2-oxobutanoate (2-oxoisovalerate) and (S)-3-methyl-2-oxopentanoate and are known as the branched-chain alpha-ketoacid dehydrogenases. They can pass electrons to NAD (EC 1.2.1.25) or to ferredoxin (EC 1.2.7.7). The NAD-dependent enzyme is the sum of three activities: EC 1.2.4.4 (the 4-methyl-2-oxopentanoate dehydrogenase, with transfer to the lipopoyllysine residue of 2.3.1.168) which is itself heteromeric, with alpha and beta subunits; EC 2.3.1.168 (dihydrolipoyllysine-residue (3-methylbutanoyl)transferase); and EC 1.8.1.4 (dihydrolipoyl dehydrogenase, transferring electrons to NAD). The well-characterized ferredoxin-dependent enzymes have 3 subunits (vorABC) or 2 subunits (ofoAB). Genetic data identified a fused ferredoxin-dependent enzyme with just 1 subunit (ofo).

propionyl-CoA-degradation:

prpC, prpD, acn and prpB
or prpC, acnD, prpF, acn and prpB
or propionyl-CoA-carboxylase, epi and methylmalonyl-CoA-mutase
or pco, hpcD, dddA and iolA
Comment: In 2-methylcitrate cycle I, propionyl-CoA is combined with oxalacetate (by prpC) to give methylcitrate, dehydrated to cis-2-methylaconitate by prpD, hydrated to (2R,3S)-2-methylisocitrate, and a lyase produces pyruvate and succinate. (We consider succinate as a central intermediate, as most organisms can activate it to succinyl-CoA or can oxidize it to fumarate and convert that to oxaloacetate.) In 2-methylcitrate cycle II, a different dehydratase (acnD) and an isomerase (prpF) replace the dehydratase prpD; acnD dehydrates (2S,3S)-2-methylcitrate to 2-methyl-trans-aconitate, and prpF isomerizes it to cis-2-methylaconitate. In propanoyl CoA degradation I, propionyl-CoA carboxylase forms (S)-methylmalonyl-CoA, methylmalonyl-CoA epimerase forms (R)-methylmalonyl-CoA, and methylmalonyl-CoA mutase forms succinyl-CoA, which is a central metabolite. (Note that methylmalonyl-CoA mutase requires adenosylcobamide, a form of vitamin B12, for activity.) In propanoyl-CoA degradation II: propionyl-CoA is oxidized to acrylyl-CoA by pco, hydrated to 3-hydroxypropionyl-CoA, hydrolzed to 3-hydroxypropionate, oxidized to 3-oxopropionate (malonate semialdehyde), and oxidized to acetyl-CoA and CO2.

methylmalonyl-CoA-mutase:

mcmA
or mcm-large and mcm-small
Comment: methylmalonyl-CoA mutase has a catalytic domain and a B12-binding domain. These are usually found in the same protein, which we call mcmA. In Metallosphaera and Pyrococcus, the B12-binding domain is a separate subunit. In Propionibacterium and Methylorubrum, there is an additional subunit with a catalytic domain only; this may have a protective role (PMID:14734568) and is not described here. There's also a mcm-interacting GTPase (known as MeaB or YgfD) that loads B12 onto mcm and protects it from inactivation (see PMC4631608); this is not described here. Some fused mcm proteins include a MeaB domain as well (i.e., Q8F222, Q8Y2U5).

propionyl-CoA-carboxylase:

pccA and pccB
or pccA1, pccA2 and pccB
Comment: propionyl-CoA carboxylase is a heteromer, usually with alpha and beta subunits pccAB. Haloferax mediterranei has a third subunit as well (pccX), which is not described here. Acidianus brierleyi has a diverged pccA split into two pieces.

valine-transport:

livF, livG, livJ, livH and livM
or natA, natB, natC, natD and natE
or Bap2
or brnQ
or phtJ
or bcaP
Comment: Transporters were identified using query: transporter:valine:L-valine:val

47 steps (32 with candidates)

Or see definitions of steps

Step	Description	Best candidate	2nd candidate
livF	L-valine ABC transporter, ATPase component 1 (LivF/BraG)	BWI76_RS26330	BWI76_RS07265
livG	L-valine ABC transporter, ATPase component 2 (LivG/BraF)	BWI76_RS26335	BWI76_RS05980
livJ	L-valine ABC transporter, substrate-binding component (LivJ/LivK/BraC/BraC3)	BWI76_RS26365	BWI76_RS26350
livH	L-valine ABC transporter, permease component 1 (LivH/BraD)	BWI76_RS26345	BWI76_RS05990
livM	L-valine ABC transporter, permease component 2 (LivM/BraE)	BWI76_RS26340	BWI76_RS07275
ofo	branched-chain alpha-ketoacid:ferredoxin oxidoreductase, fused
acdH	isobutyryl-CoA dehydrogenase
ech	(S)-3-hydroxybutanoyl-CoA hydro-lyase	BWI76_RS13115	BWI76_RS20455
bch	3-hydroxyisobutyryl-CoA hydrolase	BWI76_RS13120	BWI76_RS20065
mmsB	3-hydroxyisobutyrate dehydrogenase	BWI76_RS24825	BWI76_RS07000
mmsA	methylmalonate-semialdehyde dehydrogenase	BWI76_RS03070	BWI76_RS02840
prpC	2-methylcitrate synthase	BWI76_RS08385	BWI76_RS19140
acnD	2-methylcitrate dehydratase (2-methyl-trans-aconitate forming)	BWI76_RS11940
prpF	methylaconitate isomerase	BWI76_RS04370	BWI76_RS14180
acn	(2R,3S)-2-methylcitrate dehydratase	BWI76_RS04910	BWI76_RS11940
prpB	2-methylisocitrate lyase	BWI76_RS01665
Alternative steps:
Bap2	L-valine permease Bap2	BWI76_RS19685	BWI76_RS07360
bcaP	L-valine uptake transporter BcaP/CitA
bkdA	branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit	BWI76_RS14160
bkdB	branched-chain alpha-ketoacid dehydrogenase, E1 component beta subunit	BWI76_RS14155
bkdC	branched-chain alpha-ketoacid dehydrogenase, E2 component	BWI76_RS04885	BWI76_RS14150
brnQ	L-valine:cation symporter BrnQ/BraZ/BraB	BWI76_RS06240
dddA	3-hydroxypropionate dehydrogenase	BWI76_RS07610
epi	methylmalonyl-CoA epimerase
hpcD	3-hydroxypropionyl-CoA dehydratase	BWI76_RS13115	BWI76_RS13120
iolA	malonate semialdehyde dehydrogenase (CoA-acylating)	BWI76_RS03070	BWI76_RS02840
lpd	branched-chain alpha-ketoacid dehydrogenase, E3 component	BWI76_RS04890	BWI76_RS14145
mcm-large	methylmalonyl-CoA mutase, large (catalytic) subunit	BWI76_RS23935
mcm-small	methylmalonyl-CoA mutase, small (adenosylcobamide-binding) subunit	BWI76_RS23935
mcmA	methylmalonyl-CoA mutase, fused catalytic and adenosylcobamide-binding components	BWI76_RS23935
natA	L-valine ABC transporter, ATPase component 1 (NatA)	BWI76_RS26335	BWI76_RS07270
natB	L-valine ABC transporter, substrate-binding component NatB
natC	L-valine ABC transporter, permease component 1 (NatC)	BWI76_RS05985
natD	L-valine ABC transporter, permease component 2 (NatD)	BWI76_RS05990	BWI76_RS26345
natE	L-valine ABC transporter, ATPase component 2 (NatE)	BWI76_RS26330	BWI76_RS05975
ofoA	branched-chain alpha-ketoacid:ferredoxin oxidoreductase, alpha subunit OfoA
ofoB	branched-chain alpha-ketoacid:ferredoxin oxidoreductase, beta subunit OfoB
pccA	propionyl-CoA carboxylase, alpha subunit	BWI76_RS25540	BWI76_RS13985
pccA1	propionyl-CoA carboxylase, biotin carboxyl carrier subunit	BWI76_RS25540	BWI76_RS13985
pccA2	propionyl-CoA carboxylase, biotin carboxylase subunit
pccB	propionyl-CoA carboxylase, beta subunit
pco	propanyl-CoA oxidase
phtJ	L-valine uptake permease PhtJ
prpD	2-methylcitrate dehydratase
vorA	branched-chain alpha-ketoacid:ferredoxin oxidoreductase, alpha subunit VorA
vorB	branched-chain alpha-ketoacid:ferredoxin oxidoreductase, beta subunit VorB
vorC	branched-chain alpha-ketoacid:ferredoxin oxidoreductase, gamma subunit VorC

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources