Alignments for a candidate for ilvC in Bacteroides fluxus YIT 12057

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvC in Bacteroides fluxus YIT 12057

Align Ketol-acid reductoisomerase (NAD(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; EC 1.1.1.382 (characterized)
to candidate WP_009124397.1 HMPREF9446_RS05230 ketol-acid reductoisomerase

Query= SwissProt::A8ZTR0
         (352 letters)



>NCBI__GCF_000195635.1:WP_009124397.1
          Length = 347

 Score =  459 bits (1181), Expect = e-134
 Identities = 225/347 (64%), Positives = 273/347 (78%), Gaps = 1/347 (0%)

Query: 1   MPTINFGGVEENVVTSEEFTLKKAREVLKNEVITVLGYGVQGPAQALNLKDNGFEVIIGQ 60
           M  +NFGGV ENV+T EEF L+KARE+LK+E I V+GYGVQGP QA NL+DNGF VI+GQ
Sbjct: 1   MAQLNFGGVIENVMTREEFPLEKAREILKDETIAVIGYGVQGPGQACNLRDNGFNVIVGQ 60

Query: 61  LEGDAYWEKAIADGFVPGKTLFPIEEAAKKGTIIKMLLSDAGQVAVWPKVKKCLKKGDAL 120
             G  Y EKAIADG+VPG+TLF IEEA +KGT+I  LLSDA  ++VWP +K  L  G AL
Sbjct: 61  RPGKTY-EKAIADGWVPGETLFGIEEACQKGTVIMCLLSDAAVMSVWPVMKPYLTAGKAL 119

Query: 121 YFSHGFGIVYKDQTGIVPPKNVDVILVAPKGSGTNVRRNFKDGSGINSSYAVFQDATGRA 180
           YFSHGF I + D+TG+VPPK++DVI+VAPKGSGT++R  F +G G+NSSYA++QDATG+A
Sbjct: 120 YFSHGFAITWSDRTGVVPPKDIDVIMVAPKGSGTSLRTMFLEGRGLNSSYAIYQDATGKA 179

Query: 181 EERTIALGIAIGSGYLFPTTFEKEVFSDLTGERGVLMGCLAGTMEAQYNVLRKHGHSPSE 240
            +RT+ALGI IGSGYLF TTF++E  SDLTGERG LMG + G + AQY VLR++GH+PSE
Sbjct: 180 LDRTLALGIGIGSGYLFETTFQREATSDLTGERGSLMGAIQGLLLAQYEVLRENGHTPSE 239

Query: 241 AFNETVEELTQSLIRLVAENGMDWMFANCSTTAQRGALDWAPKFRDAVAPVFDSLYRRVK 300
           AFNETVEELTQSL+ L A+NGMDWM+ANCSTTAQRGALDW   F DA+ PV + LY  VK
Sbjct: 240 AFNETVEELTQSLMPLFAKNGMDWMYANCSTTAQRGALDWMTPFHDAIKPVVEKLYASVK 299

Query: 301 NGAETRRVLKVNSAPNYLEKLRKELDTIKNSEMWQAGAAVRALRPEN 347
           +G E +  +  NS P+Y EKL  EL  ++ SEMWQ    VR LRPEN
Sbjct: 300 SGNEAQISIDSNSKPDYREKLNAELKALRESEMWQTAVTVRKLRPEN 346


Lambda     K      H
   0.316    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 423
Number of extensions: 11
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 352
Length of database: 347
Length adjustment: 29
Effective length of query: 323
Effective length of database: 318
Effective search space:   102714
Effective search space used:   102714
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

Align candidate WP_009124397.1 HMPREF9446_RS05230 (ketol-acid reductoisomerase)
to HMM TIGR00465 (ilvC: ketol-acid reductoisomerase (EC 1.1.1.86))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00465.hmm
# target sequence database:        /tmp/gapView.1191560.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00465  [M=314]
Accession:   TIGR00465
Description: ilvC: ketol-acid reductoisomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
    5.1e-98  313.9   0.0    5.7e-98  313.7   0.0    1.0  1  NCBI__GCF_000195635.1:WP_009124397.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000195635.1:WP_009124397.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  313.7   0.0   5.7e-98   5.7e-98       1     313 [.      28     346 ..      28     347 .] 0.96

  Alignments for each domain:
  == domain 1  score: 313.7 bits;  conditional E-value: 5.7e-98
                             TIGR00465   1 lkgkkvaiiGyGsqGeaqalnlrdsglnvivglrkeaaswkkAeed....GfkvltveeaikkadlimiLlpD 69 
                                           lk++++a+iGyG qG +qa nlrd+g nvivg r++ ++++kA+ d    G  ++ +eea +k+ +im Ll+D
  NCBI__GCF_000195635.1:WP_009124397.1  28 LKDETIAVIGYGVQGPGQACNLRDNGFNVIVGQRPG-KTYEKAIADgwvpGETLFGIEEACQKGTVIMCLLSD 99 
                                           799*****************************9998.9********8888899******************** PP

                             TIGR00465  70 evqkevyeaeikpllkegkallfsHGfnivfkqiv...ipkdvdvvlvAPKgpGalvReeykegrGvpsliAv 139
                                           ++  +v+   +kp+l++gkal+fsHGf i +++ +   +pkd+dv++vAPKg+G+ +R ++ egrG++s++A+
  NCBI__GCF_000195635.1:WP_009124397.1 100 AAVMSVWPV-MKPYLTAGKALYFSHGFAITWSDRTgvvPPKDIDVIMVAPKGSGTSLRTMFLEGRGLNSSYAI 171
                                           *******99.*******************997654444*********************************** PP

                             TIGR00465 140 eqdvtgeakeiAlayAkaiGgaragvlettFkeEvesDLfGEqavLcGglealikaafdtLveaGyqpelAyf 212
                                           +qd+tg+a +  la+  +iG++  +++ettF++E  sDL GE++ L+G +++l++a++++L+e+G+ p++A++
  NCBI__GCF_000195635.1:WP_009124397.1 172 YQDATGKALDRTLALGIGIGSG--YLFETTFQREATSDLTGERGSLMGAIQGLLLAQYEVLRENGHTPSEAFN 242
                                           ********************99..************************************************* PP

                             TIGR00465 213 eivhel.klivdllkekGlelmrdavsntAklgalelreilkeelkkemqkilkeiqnGefakewalekeagk 284
                                           e+v+el +++++l  ++G++ m+ ++s+tA++gal++   +++++k++++k++ ++++G++a+  ++++ ++k
  NCBI__GCF_000195635.1:WP_009124397.1 243 ETVEELtQSLMPLFAKNGMDWMYANCSTTAQRGALDWMTPFHDAIKPVVEKLYASVKSGNEAQ-ISIDS-NSK 313
                                           ******9*******************************************************9.66665.9** PP

                             TIGR00465 285 pafeearkke....keqeiekvGkelralvkae 313
                                           p+++e+ ++e    +e e++++ +++r+l++++
  NCBI__GCF_000195635.1:WP_009124397.1 314 PDYREKLNAElkalRESEMWQTAVTVRKLRPEN 346
                                           *****999999999**************99875 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (314 nodes)
Target sequences:                          1  (347 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 14.98
//
[ok]

This GapMind analysis is from Jul 26 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the original publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis