GapMind for Amino acid biosynthesis

 

Alignments for a candidate for aroE in Thermophagus xiamenensis HS1

Align shikimate dehydrogenase (EC 1.1.1.25) (characterized)
to candidate WP_010528856.1 GQW_RS0115955 shikimate dehydrogenase

Query= reanno::Btheta:353741
         (248 letters)



>NCBI__GCF_000220155.1:WP_010528856.1
          Length = 248

 Score =  284 bits (727), Expect = 1e-81
 Identities = 147/249 (59%), Positives = 184/249 (73%), Gaps = 3/249 (1%)

Query: 1   MEKYGLIGYPLRHSFSIGYFNEKFRSEGINAEYVNFEIPNINDFMEVIEENPNLCGLNVT 60
           M+ +GLIGYPL HSFS  YF EKF+ E I+A Y+NF IP IN+  ++++++P L GLNVT
Sbjct: 1   MQTFGLIGYPLGHSFSQSYFTEKFKKENIDARYLNFPIPEINELPDLLKKHPYLAGLNVT 60

Query: 61  IPYKEQVIPFLNELDRDTAKIGAVNVIKIIRQPKGKVK-LVGYNSDIIGFTQSIQPLLQP 119
           IPYK+QVI +L+ELD     IGAVNVIKI    K KV  L GYNSD+IGF++SI PLL+P
Sbjct: 61  IPYKQQVIDYLDELDPQAKDIGAVNVIKITW--KNKVPYLKGYNSDVIGFSRSITPLLKP 118

Query: 120 QHKKALILGTGGASKAVYHGLKNLGIESVFVSRTHKTDDMLTYEELTPEIMEEYTVIVNC 179
            H KALILGTGGASKAV + LK LG+   FVSR  +    ++Y+ LTPEI++EY VI+N 
Sbjct: 119 LHTKALILGTGGASKAVAYALKQLGLLFRFVSRNPQHPSHVSYQALTPEIIKEYKVIINT 178

Query: 180 TPVGMYPKVDFCPNIPYELLTPNHLLYDLLYNPNVTLFMKKGEAQGAVTKNGLEMLLLQA 239
           TP+GM P  D  P IPYE +T  HL +DL+YNP  TLF+ K   +GAV KNGLEML LQA
Sbjct: 179 TPLGMAPNTDVAPPIPYEGITQEHLAFDLIYNPETTLFLNKCANKGAVIKNGLEMLHLQA 238

Query: 240 FAAWEIWHR 248
            AAWEI+++
Sbjct: 239 EAAWEIFNQ 247


Lambda     K      H
   0.320    0.140    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 263
Number of extensions: 8
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 248
Length of database: 248
Length adjustment: 24
Effective length of query: 224
Effective length of database: 224
Effective search space:    50176
Effective search space used:    50176
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Jul 26 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory