Align branched-chain-amino-acid transaminase (EC 2.6.1.42) (characterized)
to candidate WP_010529812.1 ON01_RS04515 D-amino-acid transaminase
Query= BRENDA::E6TUA8 (302 letters) >NCBI__GCF_000224785.1:WP_010529812.1 Length = 287 Score = 142 bits (359), Expect = 7e-39 Identities = 85/272 (31%), Positives = 147/272 (54%), Gaps = 7/272 (2%) Query: 6 IFSNGKFVTKQEAVISVYDHGFLYGDGVFEGIRVYDGNIFKLTEHLKRLYESAQSIMLEI 65 I S F + + G +GDG++E IR+Y G + +TEH+ RL+ SA++I +++ Sbjct: 7 ILSQADFTNRDSLTYPFEERGLQFGDGIYEVIRIYQGAYYLITEHVNRLFRSAEAIKIDL 66 Query: 66 PYSKEDFQQIIVDTVRKNQLES-GYIRVVVSRGPGNLGLDPSRCSAPHVIVIAEELALFP 124 P+S+++ ++ D + +N++ S G + + +RG + +V +++ P Sbjct: 67 PFSRQEITSLLHDLLSQNEMTSDGIVYLQATRGSAPRNHAFPVDTPANVYAYVQDM---P 123 Query: 125 KELYELGLTVASVASRRNRPDVLSPQIKSLNYLNNILVKLEANQAGAHEALMLNDQGYVT 184 ++ L V+++ R R + + IKSLN L N+L K A + G++EA++ D G VT Sbjct: 124 RKTKNLTNGVSAITKRDTRWE--NCYIKSLNLLPNVLAKQAAQENGSYEAILERD-GKVT 180 Query: 185 EGSADNIFIVKNNTIITPPVYLGALEGITRNAIIDLAKECGYEMKETPFTRHDVYVADEV 244 E S+ N+++VK+ I T P L G R + A E F+ D+ ADEV Sbjct: 181 ECSSSNVYLVKDGKIYTHPATNRILHGCVRMRVEQFAAGLNIPFVEQAFSIEDIADADEV 240 Query: 245 FLTGTAAEVIAVVEVDKRMISDGKPGKVTNHL 276 FL+ + +EV+ +VEVD + I++G PGK T L Sbjct: 241 FLSSSTSEVMPIVEVDGKKIAEGIPGKTTQML 272 Lambda K H 0.318 0.136 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 236 Number of extensions: 10 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 302 Length of database: 287 Length adjustment: 26 Effective length of query: 276 Effective length of database: 261 Effective search space: 72036 Effective search space used: 72036 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Jul 26 2024. The underlying query database was built on Jul 25 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory