Alignments for a candidate for lysJ in Streptacidiphilus oryzae TH49

GapMind for Amino acid biosynthesis

Alignments for a candidate for lysJ in Streptacidiphilus oryzae TH49

Align [LysW]-aminoadipate semialdehyde transaminase; EC 2.6.1.118 (uncharacterized)
to candidate WP_037574263.1 BS73_RS19430 aminotransferase class III-fold pyridoxal phosphate-dependent enzyme

Query= curated2:Q9RW75
         (429 letters)



>NCBI__GCF_000744815.1:WP_037574263.1
          Length = 425

 Score =  231 bits (589), Expect = 3e-65
 Identities = 151/398 (37%), Positives = 210/398 (52%), Gaps = 39/398 (9%)

Query: 28  VVMVRGQGATVWDENGRSYIDCVVGYGVATLGHSHPDVVKAVQEQAGKLMVMPQTVPNDK 87
           V+  RG+G  +++E+GR ++D   G GV + GH HP VV A QEQ G+L+    T    K
Sbjct: 14  VLAERGEGVYLYEESGRRHLDFTAGIGVTSTGHCHPKVVAAAQEQVGRLVHGQYTTVMHK 73

Query: 88  RAEFLQELVG-VLPQGLDRVFLCNSGTEAMEAAKKFAITATGRSRFVSMKRGFSGRSLGA 146
               L E +G VLP GLD +F  NSG+EA+EAA + A  AT R   V  +  F GR+L A
Sbjct: 74  PLLTLSERLGEVLPAGLDSLFFVNSGSEAVEAAMRLAKQATARPNIVVFQGSFHGRTLAA 133

Query: 147 LSFTWEP-KYREPFGDAVDNKSV-------DFVTYG------------NLDELRAAVTE- 185
            S T    K+R  +G  +   ++        +  YG             LDEL A V+  
Sbjct: 134 ASMTTSATKFRSGYGPLMPGVAIAPFPHAAHYARYGMDEEAATRFALRELDELLATVSAP 193

Query: 186 -QTAAVIMEPVQGEGGVRPASAEFIQEARRITREKGALLILDEIQTGFCRTGKMFACEHF 244
             TAA I+EPV GEGG  PA++ F++  R      G LLILDE+QTGF RTG+ +  +HF
Sbjct: 194 ADTAAFIVEPVLGEGGYVPANSAFLRGLRERADRHGILLILDEVQTGFGRTGRFWGHDHF 253

Query: 245 GVIPDGMTLAKAIAGGTPTAAFAMMSEVADRMPAGGHGTTFGGNPLSMAAGVASLRAMKR 304
              PD +  AK +A G P +A A    + ++   G  G T+GGN ++ AA +A+L  ++ 
Sbjct: 254 DGRPDILVTAKGLASGFPLSAIAAPRALMEKAWPGSQGGTYGGNAVACAAAIATLDVIQE 313

Query: 305 EGLAEQAREKGAYMMDKLRAIQSPK-------IREVRGLGLMIGVELKE-------KSAP 350
           E L E A E+G  +   LR + +         I +VRGLGLM G E           +A 
Sbjct: 314 EKLVENAAERGEQLFAGLREVAAAHPAGGRGGIGDVRGLGLMAGTEFTTADGEPDGATAA 373

Query: 351 YIHAMEHDEGVLCLAATPL--VVRFLPPAVISKEQIDQ 386
            +HA   + G+L L   P   VVR +PP V+  EQID+
Sbjct: 374 KVHAAAAERGLLLLTCGPRGEVVRMIPPLVVDAEQIDE 411


Lambda     K      H
   0.317    0.132    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 485
Number of extensions: 26
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 429
Length of database: 425
Length adjustment: 32
Effective length of query: 397
Effective length of database: 393
Effective search space:   156021
Effective search space used:   156021
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Jul 26 2024. The underlying query database was built on Jul 25 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the original publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis