Alignments for a candidate for leuC in Novosphingobium barchaimii LL02

GapMind for Amino acid biosynthesis

Alignments for a candidate for leuC in Novosphingobium barchaimii LL02

Align 3-isopropylmalate dehydratase large subunit; EC 4.2.1.33 (characterized)
to candidate WP_059151347.1 V474_RS10030 3-isopropylmalate dehydratase large subunit

Query= CharProtDB::CH_024771
         (466 letters)



>NCBI__GCF_001046635.1:WP_059151347.1
          Length = 460

 Score =  422 bits (1086), Expect = e-123
 Identities = 223/466 (47%), Positives = 288/466 (61%), Gaps = 17/466 (3%)

Query: 2   AKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGLRAHGRPVRQPGKTFATM 61
           AKTL+EK++DAH V +      L+ +DR  +HE T   A   + A  RPV  P + FA M
Sbjct: 6   AKTLFEKIWDAHHVADTATGASLIAVDRVFLHERTGAAALKSMAAAKRPVCDPARVFAVM 65

Query: 62  DHNVSTQT-KDINACGEMARIQMQELIKNCKEFGVELYDLNHPYQGIVHVMGPEQGVTLP 120
           DH V T+  +      E     + E     K  G+ L+D+N   QGI HV+ PE G+ LP
Sbjct: 66  DHIVDTRPGRGDGTLMEGGSAFITETRAAAKAAGITLFDVNDRDQGITHVISPELGIVLP 125

Query: 121 GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVQGKAAPGITA 180
           G+T+V  DSHT T GA GALA+GIG+SE EH +AT  L+  R KTM++  +GK APG+TA
Sbjct: 126 GLTLVAPDSHTCTQGALGALAWGIGSSEAEHAMATGVLRLDRPKTMRVTYRGKLAPGVTA 185

Query: 181 KDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVAPDETTF 240
           KD+VLA+I   G+ GG GHVVEF GEA+R L +E RMTLCNMA E  A  G++APD  TF
Sbjct: 186 KDMVLALIAANGAGGGAGHVVEFAGEAVRALDIEARMTLCNMATEFAAMTGIIAPDARTF 245

Query: 241 NYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEISPQVTWGTNPGQVISV 300
            Y+  R +AP  ++FD+   YW TL+TD+GA FD  + + A  ++P V+WGT+P   + V
Sbjct: 246 EYLADRRYAP--ENFDE--PYWHTLRTDDGARFDREIEIDAAALAPMVSWGTSPEHAMPV 301

Query: 301 NDNIPDPASFADPVERASAEKALAYMGLKPGIPLTEVAIDKVFIGSCTNSRIEDLRAAAE 360
           +  +P               +A  Y+GL  G PL    ID  FIGSCTN+RI DLR AA 
Sbjct: 302 DGTVPQ-----------GPPRAHDYIGLDAGAPLLGTPIDVAFIGSCTNARISDLRRAAA 350

Query: 361 IAKGRKVAPGV-QALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLN 419
           + KGR +AP + +ALVVPGS  VK  AEAEGLD IF  AGFEWR  GCS+C     +   
Sbjct: 351 MLKGRHIAPSIRKALVVPGSSAVKRAAEAEGLDAIFTAAGFEWRASGCSLCFFAGGESFP 410

Query: 420 PGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRNI 465
            G R  S++NRNFEGRQG G RTH+ SP    A+A+ G  AD R +
Sbjct: 411 EGSRTVSSTNRNFEGRQGPGIRTHIASPETVVASAIAGMIADPREL 456


Lambda     K      H
   0.317    0.134    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 657
Number of extensions: 25
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 466
Length of database: 460
Length adjustment: 33
Effective length of query: 433
Effective length of database: 427
Effective search space:   184891
Effective search space used:   184891
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Jul 26 2024. The underlying query database was built on Jul 25 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the original publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis