Alignments for a candidate for argC in Novosphingobium barchaimii LL02

GapMind for Amino acid biosynthesis

Alignments for a candidate for argC in Novosphingobium barchaimii LL02

Align N-acetylglutamylphosphate reductase (EC 1.2.1.38) (characterized)
to candidate WP_059151924.1 V474_RS13245 N-acetyl-gamma-glutamyl-phosphate reductase

Query= reanno::Korea:Ga0059261_0351
         (310 letters)



>NCBI__GCF_001046635.1:WP_059151924.1
          Length = 307

 Score =  347 bits (891), Expect = e-100
 Identities = 187/311 (60%), Positives = 222/311 (71%), Gaps = 5/311 (1%)

Query: 1   MSVSVFIDGAAGTTGLEIRERLATRSDISLIGLDDAHRKDAGKRAEAINSADFVILCLPD 60
           M+ SVFIDGAAGTTGLEI +RLA RS+  L+ L D  RKD   RAEA+N+ADFVILCLPD
Sbjct: 1   MTASVFIDGAAGTTGLEIADRLAGRSEFELLVLPDDRRKDDAARAEALNAADFVILCLPD 60

Query: 61  DAAREAVALIANDTTRVIDASTAHRVADGWTYGFAELEPAQAAAIAEARYVSNPGCWPTG 120
           DAA+ AV +IAND TRVIDAS+A R A  WTYGF E+      A+A A  VSNPGC+P+G
Sbjct: 61  DAAKAAVGMIANDRTRVIDASSAFRTAPDWTYGFPEI--VGRDAVASAMRVSNPGCYPSG 118

Query: 121 FLALVRPLVRAGLIPPDWVLTAGGASGYSGGGKSMIAEFEDGSEPTGFRAYGLDLEHKHL 180
           F+ALV PL+RAGL+P  W       SGYSGGGK++IA FE  ++   FRAYGL L HKH+
Sbjct: 119 FIALVAPLIRAGLLPAQWPYVCHAVSGYSGGGKALIARFEADTD-IAFRAYGLALGHKHV 177

Query: 181 PEMQKHARIDHAPIFQPAVARSYRGMLIEVPLPLQMLPRKPAPAQIEQVLADAYRDSPIV 240
           PEM  HA + HAPIF PAV  ++RGML+EVPL L+M+P    P  +   L D Y DSP+V
Sbjct: 178 PEMLVHAGLTHAPIFAPAVVPAHRGMLVEVPLHLEMMPGAGTPEALRTALTDFYADSPVV 237

Query: 241 RVL-PSDDVSLVRIEDDAGTDRLTIRVFGNAERGQARLVATLDNLGKGAGGAAVQNLNIM 299
            V     D  L+R   D   D LT+RV G+A+  QARLVA LDNLGKGA GAAVQNLNIM
Sbjct: 238 NVAGDQPDELLLRAAMDP-VDSLTLRVLGSADGTQARLVAMLDNLGKGASGAAVQNLNIM 296

Query: 300 AGFDQTAGLVL 310
           AG ++TAGL L
Sbjct: 297 AGLNETAGLRL 307


Lambda     K      H
   0.319    0.136    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 335
Number of extensions: 16
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 310
Length of database: 307
Length adjustment: 27
Effective length of query: 283
Effective length of database: 280
Effective search space:    79240
Effective search space used:    79240
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

Align candidate WP_059151924.1 V474_RS13245 (N-acetyl-gamma-glutamyl-phosphate reductase)
to HMM TIGR01851 (argC: N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01851.hmm
# target sequence database:        /tmp/gapView.459332.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01851  [M=310]
Accession:   TIGR01851
Description: argC_other: N-acetyl-gamma-glutamyl-phosphate reductase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   1.5e-112  361.9   0.0   1.7e-112  361.7   0.0    1.0  1  NCBI__GCF_001046635.1:WP_059151924.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_001046635.1:WP_059151924.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  361.7   0.0  1.7e-112  1.7e-112       3     310 .]       4     299 ..       2     299 .. 0.96

  Alignments for each domain:
  == domain 1  score: 361.7 bits;  conditional E-value: 1.7e-112
                             TIGR01851   3 kvfidGeaGttGlqirerlaerddlellsidedkrkdaderakllnaadvailclpddaareavslvdnpntk 75 
                                            vfidG aGttGl+i +rla+r+++ell + +d+rkd ++ra+ lnaad +ilclpddaa+ av ++ n +t+
  NCBI__GCF_001046635.1:WP_059151924.1   4 SVFIDGAAGTTGLEIADRLAGRSEFELLVLPDDRRKDDAARAEALNAADFVILCLPDDAAKAAVGMIANDRTR 76 
                                           69*********************************************************************** PP

                             TIGR01851  76 ildastayrtaedwvyGfpelaaeqrekianakrvanPGcyatgaiallrPlveaGilPadfPvtinavsGys 148
                                           ++das+a+rta+dw+yGfpe+    r+ +a+a rv+nPGcy++g+ial++Pl+ aG+lPa++P   +avsGys
  NCBI__GCF_001046635.1:WP_059151924.1  77 VIDASSAFRTAPDWTYGFPEIVG--RDAVASAMRVSNPGCYPSGFIALVAPLIRAGLLPAQWPYVCHAVSGYS 147
                                           ********************975..899********************************************* PP

                             TIGR01851 149 GGGkaliakyeeesaddskkaafiiyglalehkhlpemrkhsglaskPiftPavGdfaqGllveiplhlaeld 221
                                           GGGkalia++e     + ++ af++yglal+hkh+pem +h+gl++ Pif Pav   ++G+lve+plhl+ + 
  NCBI__GCF_001046635.1:WP_059151924.1 148 GGGKALIARFE-----ADTDIAFRAYGLALGHKHVPEMLVHAGLTHAPIFAPAVVPAHRGMLVEVPLHLEMMP 215
                                           ***********.....44579**************************************************** PP

                             TIGR01851 222 skvsaedihkalaeyykGekfvkvaelddaellddtildaqglngtnrlelfvfgsddgerallvarldnlGk 294
                                           ++ ++e++  al+++y+    v+va  +  el     l   +++  + l l v gs+dg +a+lva ldnlGk
  NCBI__GCF_001046635.1:WP_059151924.1 216 GAGTPEALRTALTDFYADSPVVNVAGDQPDEL-----LLRAAMDPVDSLTLRVLGSADGTQARLVAMLDNLGK 283
                                           ************************98665554.....55666777899************************* PP

                             TIGR01851 295 GasGaavqnlnialGl 310
                                           GasGaavqnlni+ Gl
  NCBI__GCF_001046635.1:WP_059151924.1 284 GASGAAVQNLNIMAGL 299
                                           *************997 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (310 nodes)
Target sequences:                          1  (307 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 14.50
//
[ok]

This GapMind analysis is from Jul 26 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the original publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis