Align Histidine phosphatase family protein (characterized, see rationale)
to candidate WP_059152825.1 V474_RS18260 2,3-diphosphoglycerate-dependent phosphoglycerate mutase
Query= uniprot:A0A3D8VK77 (213 letters) >NCBI__GCF_001046635.1:WP_059152825.1 Length = 228 Score = 61.2 bits (147), Expect = 1e-14 Identities = 59/191 (30%), Positives = 85/191 (44%), Gaps = 28/191 (14%) Query: 2 RILLARHGETPWNAEGRYQGQEDIPLSDVGIGQARALGERLRDVQITRAVA--SPLGRAR 59 R++L RHG++ WN E R+ G D+ +++ G +ARA G L+D + VA S RA Sbjct: 3 RLILLRHGQSQWNLENRFTGWWDVDVTEKGEAEARAAGALLKDKGMLPDVAFTSLQSRAI 62 Query: 60 QTAEFAL---GDRDLPLTLDPGLMEIAHGTWEGLLATEIRERDPE-RLQAWR---DTPHE 112 +T AL G +P T D L E +G GL E + E +++ WR D P Sbjct: 63 KTLHLALEAAGILWIPETKDWRLNERHYGGLTGLDKAETAAKHGEDQVKIWRRSFDIPPP 122 Query: 113 VL------------------MPEGESLQHVFDRAWPAF-ARAAEGLGPSDTLLVVAHDAV 153 V+ +P ESL+ R P + L T+LV AH Sbjct: 123 VMERGTEFDLSTDPRYAGIDVPMTESLKDTIARVLPYYEGSIVPHLKTGSTVLVAAHGNS 182 Query: 154 NRVLLCHVLGI 164 R L+ H+ GI Sbjct: 183 LRALVKHLSGI 193 Lambda K H 0.322 0.139 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 143 Number of extensions: 8 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 213 Length of database: 228 Length adjustment: 22 Effective length of query: 191 Effective length of database: 206 Effective search space: 39346 Effective search space used: 39346 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 45 (21.9 bits)
This GapMind analysis is from Jul 26 2024. The underlying query database was built on Jul 25 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory