Alignments for a candidate for tpiA in Marivita geojedonensis DPG-138

GapMind for Amino acid biosynthesis

Alignments for a candidate for tpiA in Marivita geojedonensis DPG-138

Align Triosephosphate isomerase; TIM; TPI; Triose-phosphate isomerase; EC 5.3.1.1 (characterized)
to candidate WP_085635818.1 MGEO_RS06080 triose-phosphate isomerase

Query= SwissProt::Q8L1Z5
         (254 letters)



>NCBI__GCF_002115805.1:WP_085635818.1
          Length = 245

 Score =  253 bits (645), Expect = 3e-72
 Identities = 136/246 (55%), Positives = 171/246 (69%), Gaps = 13/246 (5%)

Query: 6   RPFIAGNWKMNGTGESLGELRA---IAAGISSDLGRLFEALICVPATLLSRAFDILGGEN 62
           R   AGNWKMNGT  +L EL A   +A G +       E LIC P+TL+++A    G   
Sbjct: 3   RKLAAGNWKMNGTTAALEELDALQEVAEGAA-------EVLICPPSTLIAQAAVRSG--K 53

Query: 63  ILLGGQNCHFDDYGPYTGDISAFMLKEAGASHVIIGHSERRTVYQESDAIVRAKVQAAWR 122
           + +GGQ+CH    G +TGDISA ML++AGA+HVI+GHSERR  + ESDA+V  K  AAW 
Sbjct: 54  VAIGGQDCHSAASGAHTGDISALMLRDAGATHVILGHSERRADHGESDALVAYKALAAWD 113

Query: 123 AGLVALICVGETLEERKSNKVLDVLTRQLEGSLPDGATAENIIIAYEPVWAVGTGNTATS 182
           AGL A++CVGETLE+R+S K LDV+ +QL GSLPD  TA N +IAYEPVWA+GTG   T+
Sbjct: 114 AGLTAIVCVGETLEQRESGKTLDVIGKQLAGSLPDDVTASNTVIAYEPVWAIGTGKVPTT 173

Query: 183 ADVAEVHAFIHHKMHSRFGDEGAK-IRLLYGGSVKPSNAFELLSTAHVNGALIGGASLKA 241
             +AEVH F+   +  RFG+E A  IRLLYGGSVK +NA E+ +   V+GAL+GGASLKA
Sbjct: 174 DQIAEVHTFMRATLVQRFGNETATGIRLLYGGSVKATNATEIFAVVDVDGALVGGASLKA 233

Query: 242 IDFLTI 247
            DF  I
Sbjct: 234 SDFAPI 239


Lambda     K      H
   0.320    0.137    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 247
Number of extensions: 9
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 254
Length of database: 245
Length adjustment: 24
Effective length of query: 230
Effective length of database: 221
Effective search space:    50830
Effective search space used:    50830
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 46 (22.3 bits)

Align candidate WP_085635818.1 MGEO_RS06080 (triose-phosphate isomerase)
to HMM TIGR00419 (tpiA: triose-phosphate isomerase (EC 5.3.1.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00419.hmm
# target sequence database:        /tmp/gapView.2802952.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00419  [M=228]
Accession:   TIGR00419
Description: tim: triose-phosphate isomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
    1.3e-58  184.5   3.0    1.5e-58  184.3   3.0    1.0  1  NCBI__GCF_002115805.1:WP_085635818.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_002115805.1:WP_085635818.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  184.3   3.0   1.5e-58   1.5e-58       3     227 ..       7     233 ..       5     234 .. 0.90

  Alignments for each domain:
  == domain 1  score: 184.3 bits;  conditional E-value: 1.5e-58
                             TIGR00419   3 iinfKlnesvgkvelevaklaeevaseagvevavappfvdldvvkdeveseiqvaAqnvdavksGaftGeisA 75 
                                            +n+K+n+++   e+ +  l +eva+ a  ev + pp   +  ++ +   ++ ++ q+++   sGa+tG+isA
  NCBI__GCF_002115805.1:WP_085635818.1   7 AGNWKMNGTTAALEE-LDAL-QEVAEGAA-EVLICPPSTLIAQAAVRSG-KVAIGGQDCHSAASGAHTGDISA 75 
                                           69*******998765.5555.57998765.6667777666655443333.6********************** PP

                             TIGR00419  76 emlkdlGakgvligHsErRsllkeadeliekkvarlkelglksvvCvgetleereaartinnvattaaaaA.. 146
                                            ml+d+Ga +v++gHsErR+ + e+d l++ k   + + gl+++vCvgetle+re+++t++++ ++ a     
  NCBI__GCF_002115805.1:WP_085635818.1  76 LMLRDAGATHVILGHSERRADHGESDALVAYKALAAWDAGLTAIVCVGETLEQRESGKTLDVIGKQLAGSLpd 148
                                           ****************************************************************998876567 PP

                             TIGR00419 147 ...lepdvvAvEPveliGtGkpvskAeaevveksvrdhlkk.vskevaesvrvlyGasvtaaedaelaaqldv 215
                                               ++ v+A+EPv++iGtGk+ +  +  +v+ ++r  l +   +e a  +r+lyG+sv+a++++e +a  dv
  NCBI__GCF_002115805.1:WP_085635818.1 149 dvtASNTVIAYEPVWAIGTGKVPTTDQIAEVHTFMRATLVQrFGNETATGIRLLYGGSVKATNATEIFAVVDV 221
                                           76699*******************************98877699***************************** PP

                             TIGR00419 216 dGvLlasavlka 227
                                           dG+L+++a+lka
  NCBI__GCF_002115805.1:WP_085635818.1 222 DGALVGGASLKA 233
                                           ***********9 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (228 nodes)
Target sequences:                          1  (245 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 14.22
//
[ok]

This GapMind analysis is from Jul 26 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the original publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis