GapMind for catabolism of small carbon sources

 

Protein WP_012277060.1 in Shewanella halifaxensis HAW-EB4

Annotation: NCBI__GCF_000019185.1:WP_012277060.1

Length: 411 amino acids

Source: GCF_000019185.1 in NCBI

Candidate for 5 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-isoleucine catabolism prpF hi 2-methyl-aconitate isomerase; Cis-trans isomerase; EC 5.3.3.- (characterized) 74% 99% 578.9 3-methylitaconate isomerase; (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase; EC 5.3.3.6 37% 233.4
propionate catabolism prpF hi 2-methyl-aconitate isomerase; Cis-trans isomerase; EC 5.3.3.- (characterized) 74% 99% 578.9 3-methylitaconate isomerase; (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase; EC 5.3.3.6 37% 233.4
L-threonine catabolism prpF hi 2-methyl-aconitate isomerase; Cis-trans isomerase; EC 5.3.3.- (characterized) 74% 99% 578.9 3-methylitaconate isomerase; (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase; EC 5.3.3.6 37% 233.4
L-valine catabolism prpF hi 2-methyl-aconitate isomerase; Cis-trans isomerase; EC 5.3.3.- (characterized) 74% 99% 578.9 3-methylitaconate isomerase; (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase; EC 5.3.3.6 37% 233.4
4-hydroxybenzoate catabolism ligU lo 4-oxalomesaconate tautomerase; Gallate degradation protein D; EC 5.3.2.8 (characterized) 36% 98% 206.1 2-methyl-aconitate isomerase; Cis-trans isomerase; EC 5.3.3.- 74% 578.9

Sequence Analysis Tools

View WP_012277060.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MTTSTESKPSKASAPQIKIAATYMRGGTSKGVFFNLIDLPKEAQQAGAARDALLLRVIGS
PDPYGKQTDGMGGATSSTSKTAILAKSNKADHDVDYLFGQVAIDRPFVDWSGNCGNLTAA
VGSFAISNGLVDNSRIPDNGISVVRIWQKNIGKTIIAHVPITNGKVQETGDFELDGVTFP
AAEVQVEFLDPADGDGAMFPTGNLVDELIVPTDVVPSGVLQATMINAGIPTVFINAAELG
YTGAELQEAINGDASVLAMFEVIRAHGAVQMGLINHIDEAANRQHTPKVAIVAAPIDYTS
SSGKVIDKNTIDLNVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVSLAAGEGLSNAVNP
GFVTFGHPSGTLKVGAEASQVDGQWQVNKAVMSRSARVLMEGWVRVPADTF

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory