Align 2-hydroxymuconate-6-semialdehyde hydrolase (EC 3.7.1.9) (characterized)
to candidate WP_012277207.1 SHAL_RS11065 alpha/beta fold hydrolase
Query= BRENDA::G3KFX4 (282 letters) >NCBI__GCF_000019185.1:WP_012277207.1 Length = 278 Score = 100 bits (250), Expect = 3e-26 Identities = 81/260 (31%), Positives = 118/260 (45%), Gaps = 22/260 (8%) Query: 25 DQGEGFPVLLIHGSGPGVTAWANWRLVMPQLAQ-NRRVIAPDMLGFGYSDRPADGRYHQQ 83 DQGEG VL IHGSGPG + +N++ P + R I D+ GFG SD+P D Y Sbjct: 28 DQGEGKTVLWIHGSGPGASGHSNFKGNYPVFEKAGFRNIVLDLPGFGQSDKPDDINYDLA 87 Query: 84 RWVEHAIGVLDALGIQQADIVGNSFGGGLALALAIRHPERVRRLVLMGSVGVSFPIT--- 140 +VE L GI ++GNS GG +AL A+++P+ V +L+LM GV T Sbjct: 88 FFVETLNQFLVKTGISHCTLLGNSLGGAIALGQALKYPQSVEQLILMAPGGVEERETYFK 147 Query: 141 ----PGLDAVWGYEP-SFASMRRLMDVFAYDRS-LVTNELAELRYQASIRPGFQESFAQM 194 + ++G P MR++M + +D S L N L E A +P + F+ M Sbjct: 148 MIGIQKMVEIYGQGPMGVEEMRKVMHLQLFDTSQLDDNTLRERADVAKTQP--KNLFSTM 205 Query: 195 FPAPRQRWVDGLASDEADIRALPHETLVIHGREDQVIPLAASLTLAEWIARAQLHVFGHC 254 + + +R + L G D P A + A+ + C Sbjct: 206 L----------VPNMTDQLRLIECPILGFWGTNDNFNPPAGVDKFLDNAPNARFLLLNRC 255 Query: 255 GHWTQIEHAERFARLVENFL 274 GHW Q+EH + F R +FL Sbjct: 256 GHWIQVEHQDLFNRTCLDFL 275 Lambda K H 0.323 0.138 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 208 Number of extensions: 14 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 282 Length of database: 278 Length adjustment: 26 Effective length of query: 256 Effective length of database: 252 Effective search space: 64512 Effective search space used: 64512 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory