GapMind for catabolism of small carbon sources

 

Protein WP_012645338.1 in Geobacter daltonii FRC-32

Annotation: NCBI__GCF_000022265.1:WP_012645338.1

Length: 385 amino acids

Source: GCF_000022265.1 in NCBI

Candidate for 14 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-lysine catabolism bcd lo butanoyl-CoA dehydrogenase (NAD+, ferredoxin) (subunit 1/3) (EC 1.3.1.109); short-chain acyl-CoA dehydrogenase (subunit 1/2) (EC 1.3.8.1) (characterized) 40% 99% 297.7 Glutaryl-CoA dehydrogenase; GDH(Des); EC 1.3.99.32 46% 333.6
L-isoleucine catabolism acdH lo 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (characterized) 38% 98% 262.3 Glutaryl-CoA dehydrogenase; GDH(Des); EC 1.3.99.32 46% 333.6
L-valine catabolism acdH lo 2-methylbutanoyl-CoA dehydrogenase / butanoyl-CoA dehydrogenase / isobutyryl-CoA dehydrogenase (EC 1.3.8.1; EC 1.3.8.5) (characterized) 38% 98% 257.7 Glutaryl-CoA dehydrogenase; GDH(Des); EC 1.3.99.32 46% 333.6
L-leucine catabolism liuA lo isovaleryl-CoA dehydrogenase (EC 1.3.8.4) (characterized) 36% 92% 239.2 Glutaryl-CoA dehydrogenase; GDH(Des); EC 1.3.99.32 46% 333.6
4-hydroxybenzoate catabolism Ch1CoA lo Cyclohex-1-ene-1-carbonyl-CoA dehydrogenase; Ch1CoA; EC 1.3.8.10 (characterized) 36% 90% 233.8 Glutaryl-CoA dehydrogenase; GDH(Des); EC 1.3.99.32 46% 333.6
phenylacetate catabolism Ch1CoA lo Cyclohex-1-ene-1-carbonyl-CoA dehydrogenase; Ch1CoA; EC 1.3.8.10 (characterized) 36% 90% 233.8 Glutaryl-CoA dehydrogenase; GDH(Des); EC 1.3.99.32 46% 333.6
L-phenylalanine catabolism Ch1CoA lo Cyclohex-1-ene-1-carbonyl-CoA dehydrogenase; Ch1CoA; EC 1.3.8.10 (characterized) 36% 90% 233.8 Glutaryl-CoA dehydrogenase; GDH(Des); EC 1.3.99.32 46% 333.6
4-hydroxybenzoate catabolism gcdH lo glutaryl-CoA dehydrogenase (EC 1.3.8.6) (characterized) 32% 94% 193 Glutaryl-CoA dehydrogenase; GDH(Des); EC 1.3.99.32 46% 333.6
L-arginine catabolism gcdH lo glutaryl-CoA dehydrogenase (EC 1.3.8.6) (characterized) 32% 94% 193 Glutaryl-CoA dehydrogenase; GDH(Des); EC 1.3.99.32 46% 333.6
L-citrulline catabolism gcdH lo glutaryl-CoA dehydrogenase (EC 1.3.8.6) (characterized) 32% 94% 193 Glutaryl-CoA dehydrogenase; GDH(Des); EC 1.3.99.32 46% 333.6
L-lysine catabolism gcdH lo glutaryl-CoA dehydrogenase (EC 1.3.8.6) (characterized) 32% 94% 193 Glutaryl-CoA dehydrogenase; GDH(Des); EC 1.3.99.32 46% 333.6
phenylacetate catabolism gcdH lo glutaryl-CoA dehydrogenase (EC 1.3.8.6) (characterized) 32% 94% 193 Glutaryl-CoA dehydrogenase; GDH(Des); EC 1.3.99.32 46% 333.6
L-phenylalanine catabolism gcdH lo glutaryl-CoA dehydrogenase (EC 1.3.8.6) (characterized) 32% 94% 193 Glutaryl-CoA dehydrogenase; GDH(Des); EC 1.3.99.32 46% 333.6
L-proline catabolism gcdH lo glutaryl-CoA dehydrogenase (EC 1.3.8.6) (characterized) 32% 94% 193 Glutaryl-CoA dehydrogenase; GDH(Des); EC 1.3.99.32 46% 333.6

Sequence Analysis Tools

View WP_012645338.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MRFGLTDEQKMMQDMARDFAQKEILPTLKEDEANHTFRPELVKKMADLGFFGCALPEEYG
GNGCGFLESVIIAEQLATISGSSRLPLNMQNIGPSITVNQFGTKEQKERFIPKWVSGEYF
GFFAITEPNSGSDVISMGTTAIDRGDHWELNGQKMWISNAHLGDYGLVYAMTDKSAKYKG
MTCFIVNLKGNEGIITAPIETKLGLHCAPTGEISFSQAKIPKDSVLGEVGQGFQICMYQL
NNTRISCAAGALGIGGGAIEAAIGYANERTQFGKKIGSYQMIQASIAEMVAEHEAAKLLV
YQAAWLKDQGLPNQYQTSMAKLFASEAAVHAATETMKIFGSYGFSTEYPAERWLRDSMSL
RTVEGTSNIQKTIIAGMALGDTVNR

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory