GapMind for catabolism of small carbon sources

 

Protein WP_012645376.1 in Geobacter daltonii FRC-32

Annotation: NCBI__GCF_000022265.1:WP_012645376.1

Length: 407 amino acids

Source: GCF_000022265.1 in NCBI

Candidate for 12 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
ethanol catabolism etoh-dh-nad med alcohol dehydrogenase (EC 1.1.1.1) (characterized) 40% 71% 191 Uncharacterized zinc-type alcohol dehydrogenase-like protein AdhB; EC 1.-.-.- 35% 248.4
4-hydroxybenzoate catabolism adh lo Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 (characterized) 40% 71% 165.6 Uncharacterized zinc-type alcohol dehydrogenase-like protein AdhB; EC 1.-.-.- 35% 248.4
2'-deoxyinosine catabolism adh lo Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 (characterized) 40% 71% 165.6 Uncharacterized zinc-type alcohol dehydrogenase-like protein AdhB; EC 1.-.-.- 35% 248.4
2-deoxy-D-ribose catabolism adh lo Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 (characterized) 40% 71% 165.6 Uncharacterized zinc-type alcohol dehydrogenase-like protein AdhB; EC 1.-.-.- 35% 248.4
ethanol catabolism adh lo Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 (characterized) 40% 71% 165.6 Uncharacterized zinc-type alcohol dehydrogenase-like protein AdhB; EC 1.-.-.- 35% 248.4
L-threonine catabolism adh lo Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 (characterized) 40% 71% 165.6 Uncharacterized zinc-type alcohol dehydrogenase-like protein AdhB; EC 1.-.-.- 35% 248.4
thymidine catabolism adh lo Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 (characterized) 40% 71% 165.6 Uncharacterized zinc-type alcohol dehydrogenase-like protein AdhB; EC 1.-.-.- 35% 248.4
L-tryptophan catabolism adh lo Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 (characterized) 40% 71% 165.6 Uncharacterized zinc-type alcohol dehydrogenase-like protein AdhB; EC 1.-.-.- 35% 248.4
D-sorbitol (glucitol) catabolism sdh lo L-iditol 2-dehydrogenase; EC 1.1.1.14 (characterized) 34% 73% 147.5 Uncharacterized zinc-type alcohol dehydrogenase-like protein AdhB; EC 1.-.-.- 35% 248.4
xylitol catabolism xdhA lo L-iditol 2-dehydrogenase; EC 1.1.1.14 (characterized) 34% 73% 147.5 Uncharacterized zinc-type alcohol dehydrogenase-like protein AdhB; EC 1.-.-.- 35% 248.4
D-xylose catabolism xdhA lo L-iditol 2-dehydrogenase; EC 1.1.1.14 (characterized) 34% 73% 147.5 Uncharacterized zinc-type alcohol dehydrogenase-like protein AdhB; EC 1.-.-.- 35% 248.4
L-threonine catabolism tdh lo L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103 (uncharacterized) 32% 73% 108.6 Uncharacterized zinc-type alcohol dehydrogenase-like protein AdhB; EC 1.-.-.- 35% 248.4

Sequence Analysis Tools

View WP_012645376.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MKAVVWHGVGDIRLDDVAEPRIEQQTDAIVKITASAICGTDLHMVRGTMSGMRPGTILGH
EGVGTVEEVGTAVRNFKPGDRVLIPSTIACGYCSYCRDGYYAQCDNANPNGMQAGTSFFG
GPEKSGPVNGLQAEKARIPFAGVGLVKIPDGVTDEQAILVSDIFPTGYMGAELAEIKPGN
TVAIFGCGPVGQFAIASAKLFQAGRIFAVDCIPSRLEKAREQGAEVIDFSKEDPVQMIRD
MTGGIGCDRAIDAVGVDAEAPHTGPAVREAKEHEEEFRQEVAHVAPQTNISGDNWHPGDA
PSMTLIWGVECLAKAGTFSVIGVYPESVTLFPIGMAMNKNLTIKMGNCNHRKYIPFLLDM
VRSGTVDPVRILTRREPLTSAVDAYRAFDMRQSGWLKVELQTREMTH

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory