Alignments for a candidate for pta in Geobacter daltonii FRC-32

GapMind for catabolism of small carbon sources

Alignments for a candidate for pta in Geobacter daltonii FRC-32

Align phosphate acetyltransferase (EC 2.3.1.8) (characterized)
to candidate WP_012646712.1 GEOB_RS08080 phosphate acetyltransferase

Query= BRENDA::O52593
         (332 letters)



>NCBI__GCF_000022265.1:WP_012646712.1
          Length = 333

 Score =  341 bits (875), Expect = 1e-98
 Identities = 180/332 (54%), Positives = 241/332 (72%), Gaps = 4/332 (1%)

Query: 1   MSFLEQIIERAKSDVKTIVLPESTDLRVIKAASMIMKKGIAKVVLIGNEKEIKSLAGD-- 58
           M  +EQI  +AK +++T+VLPES D R++ AA  I+++G+AK++++GN+ E+ + A +  
Sbjct: 1   MHLMEQIKAKAKKNLQTVVLPESYDERMLFAAQKIVEQGLAKIIILGNQTEVSAAAQNKG 60

Query: 59  IDLEGVMIEDSLNSEKLEDYANTLYELRKSKGMTIEAARE--TIKDPLYYGVMMVKKGEA 116
           ++L GV I D   S KLE Y + L ELRKSKG+T E AR   T KD LYY  MMV+ G+A
Sbjct: 61  VNLAGVEILDPATSPKLEAYIDALVELRKSKGLTREEARNLLTAKDYLYYAGMMVRLGDA 120

Query: 117 DGMVAGAVNSTANTLRPALQILKTAPGTKLVSSFFVMVVPNCEYGHNGTFVYADCGLVEN 176
            G VAGA  +T N L+ A Q + TAPG K VSSFF+MV  N ++G NG  ++ADC +  N
Sbjct: 121 GGEVAGATGTTGNVLKAAFQTVGTAPGIKTVSSFFLMVTKNTDFGENGIVLFADCAVNPN 180

Query: 177 PDADQLSEIAISASKSFEMLVGAKPQVAMLSYSSYGSAKSELTEKVIKATQLAKEKAPHL 236
           PDA  L+EIA++ +++ +  +    +VAMLS+S+ GSA     +KV+KA ++A+   P L
Sbjct: 181 PDAQALAEIAVATARNCKSFLDVPARVAMLSFSTKGSAAHADVDKVLKALEIARGIDPSL 240

Query: 237 AIDGELQVDAAIVPEVAKSKAKGSSVAGKANVLIFPDLDAGNIAYKLTQRLAKAEAYGPI 296
            IDGELQ DAA++P+V + KA GS VAGKANVL+FPDLDAGNIAYKL +R+A AEA GP+
Sbjct: 241 QIDGELQADAALLPKVGEKKAPGSPVAGKANVLVFPDLDAGNIAYKLVERVAGAEAIGPV 300

Query: 297 TQGLARPVNDLSRGCSAEDIVGVAAITAVQAQ 328
            QGLA+PVNDLSRGCS +DIV VAAITAVQAQ
Sbjct: 301 IQGLAKPVNDLSRGCSVDDIVNVAAITAVQAQ 332


Lambda     K      H
   0.313    0.129    0.346 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 293
Number of extensions: 11
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 332
Length of database: 333
Length adjustment: 28
Effective length of query: 304
Effective length of database: 305
Effective search space:    92720
Effective search space used:    92720
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 49 (23.5 bits)

Align candidate WP_012646712.1 GEOB_RS08080 (phosphate acetyltransferase)
to HMM TIGR00651 (pta: phosphate acetyltransferase (EC 2.3.1.8))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00651.hmm
# target sequence database:        /tmp/gapView.3682251.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00651  [M=304]
Accession:   TIGR00651
Description: pta: phosphate acetyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   1.6e-116  375.0   0.6   1.8e-116  374.8   0.6    1.0  1  NCBI__GCF_000022265.1:WP_012646712.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000022265.1:WP_012646712.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  374.8   0.6  1.8e-116  1.8e-116       1     304 []      18     328 ..      18     328 .. 0.98

  Alignments for each domain:
  == domain 1  score: 374.8 bits;  conditional E-value: 1.8e-116
                             TIGR00651   1 ivlPEgseervlkAaallaekkiaekvllvnkeeevknkakevnlklgkvvvedpdvskdiekyverlyekrk 73 
                                           +vlPE+ +er+l Aa++++e+++a+ ++l+n+ e+ + +a++   +l+ v++ dp +s+++e y+++l+e+rk
  NCBI__GCF_000022265.1:WP_012646712.1  18 VVLPESYDERMLFAAQKIVEQGLAKIIILGNQTEVSA-AAQNKGVNLAGVEILDPATSPKLEAYIDALVELRK 89 
                                           69***********************999999988888.99999999*************************** PP

                             TIGR00651  74 hkGvtekeareql..rDevslaallvelgeadglvsGavsttaktlrpalqiiktlegvklvssvfimekee. 143
                                            kG+t++ear+ l  +D +++a ++v+lg a g+v+Ga  tt ++l++a+q ++t++g+k+vss+f+m +++ 
  NCBI__GCF_000022265.1:WP_012646712.1  90 SKGLTREEARNLLtaKDYLYYAGMMVRLGDAGGEVAGATGTTGNVLKAAFQTVGTAPGIKTVSSFFLMVTKNt 162
                                           ***********884469999**************************************************999 PP

                             TIGR00651 144 .....evlvfaDCavavdPnaeeLAeiAlqsaksakslgeeepkvallsystkgsgkgeevekvkeAvkilke 211
                                                ++++faDCav+++P+a+ LAeiA+ +a  +ks+ +   +va+ls+stkgs++ ++v+kv +A++i++ 
  NCBI__GCF_000022265.1:WP_012646712.1 163 dfgenGIVLFADCAVNPNPDAQALAEIAVATARNCKSFLDVPARVAMLSFSTKGSAAHADVDKVLKALEIARG 235
                                           999999******************************************************************* PP

                             TIGR00651 212 kepdllldGelqfDaAlvekvaekkapesevagkanvfvFPdLdaGnigYkivqRladaeaiGPilqGlakPv 284
                                             p l++dGelq DaAl++kv ekkap s+vagkanv+vFPdLdaGni+Yk+v+R+a+aeaiGP++qGlakPv
  NCBI__GCF_000022265.1:WP_012646712.1 236 IDPSLQIDGELQADAALLPKVGEKKAPGSPVAGKANVLVFPDLDAGNIAYKLVERVAGAEAIGPVIQGLAKPV 308
                                           ************************************************************************* PP

                             TIGR00651 285 nDLsRGasvedivnvviita 304
                                           nDLsRG+sv+divnv++ita
  NCBI__GCF_000022265.1:WP_012646712.1 309 NDLSRGCSVDDIVNVAAITA 328
                                           ******************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (304 nodes)
Target sequences:                          1  (333 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 10.16
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources