GapMind for catabolism of small carbon sources

 

Alignments for a candidate for natE in Geobacter daltonii FRC-32

Align NatE aka LivF aka SLR1881, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized)
to candidate WP_012647880.1 GEOB_RS13900 ABC transporter ATP-binding protein

Query= TCDB::P73650
         (240 letters)



>NCBI__GCF_000022265.1:WP_012647880.1
          Length = 247

 Score =  197 bits (502), Expect = 1e-55
 Identities = 108/229 (47%), Positives = 149/229 (65%), Gaps = 7/229 (3%)

Query: 14  YVADVPILQGINFSIAPGELVTVIGPNGAGKSTLAKTIFGLLTPSQGEIIFKGENITGLG 73
           Y   V  L+ ++  +  GE+VT+IG NGAGK+T+  TI G+   + GEI+F+ E +  L 
Sbjct: 10  YYGKVHALKNVSIHLGQGEIVTLIGANGAGKTTILNTISGVTPAAGGEILFEKEPVQALS 69

Query: 74  SDQIVRRGMCYVPQVCNVFGSLTVAENLDMGAFLHQGPTQTL------KDRIYTMFPKLA 127
            D+IVR G+  VP+   VF  L+V +NLD+GA+L     +        K  I+T+FP+L 
Sbjct: 70  PDRIVRLGIAQVPEGRQVFKPLSVEDNLDLGAYLRHRQREAKSRIRQDKQEIFTLFPRLE 129

Query: 128 QRRNQRAGTLSGGERQMLAMGRALMLDPDLLLLDEPSAALSPILVKDVFAQIKAINAT-G 186
           +RR Q AGTLSGGE+QMLA+GRA+M  P L+LLDEPS  L+P++V+++F  I  +    G
Sbjct: 130 ERRKQPAGTLSGGEQQMLAIGRAMMTRPKLMLLDEPSMGLAPLVVQEIFRVIDNLRRQHG 189

Query: 187 KAIILVEQNAKQALMMADRGYVLENGRDKLEGSGQSLLNDPLVGELYLG 235
             I+LVEQNAK AL +ADRGYVLE G+  LEG   +LL +  V   YLG
Sbjct: 190 TTILLVEQNAKAALNLADRGYVLETGKVILEGLSSNLLENKDVQRAYLG 238


Lambda     K      H
   0.320    0.139    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 163
Number of extensions: 7
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 240
Length of database: 247
Length adjustment: 23
Effective length of query: 217
Effective length of database: 224
Effective search space:    48608
Effective search space used:    48608
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory