Alignments for a candidate for aacS in Methanocella arvoryzae MRE50

GapMind for catabolism of small carbon sources

Alignments for a candidate for aacS in Methanocella arvoryzae MRE50

Align Acetoacetate--CoA ligase (EC 6.2.1.16) (characterized)
to candidate WP_012034707.1 RCI_RS01985 AMP-binding protein

Query= reanno::acidovorax_3H11:Ac3H11_3009
         (578 letters)



>NCBI__GCF_000063445.1:WP_012034707.1
          Length = 569

 Score =  657 bits (1695), Expect = 0.0
 Identities = 318/560 (56%), Positives = 403/560 (71%), Gaps = 6/560 (1%)

Query: 16  SHARGATDVPLIEQTIGAFFADMVARQPEREALVSVHQGRRYTYAQLQTEAHRLASALLG 75
           S+A   +D PLI  TIG  F ++ +  PER+ALVS HQG RYT+ + Q + +R A  LL 
Sbjct: 8   SYAYTGSDKPLIGMTIGDMFDEIASTYPERDALVSEHQGLRYTWQEFQQQVNRAAKGLLS 67

Query: 76  MGLTPGDRVGIWSHNNAEWVLMQLATAQVGLVLVNINPAYRTAEVEYALNKVGCKLLVSM 135
           MG   GDRV IW+ N AEWV+MQ ATA+VG++L+NINPAYRT E+EY L +   + LV +
Sbjct: 68  MGYKKGDRVAIWATNVAEWVIMQFATAKVGIILININPAYRTHELEYVLQQSEAQGLVLI 127

Query: 136 ARFKTSDYLGMLRELAPEWQGQQPGHLQAAKLPQLKTVVWIDDEAGQGADEPGLLRFTEL 195
             FKTSDY+ ML ++ PE +  +P ++ +  LP L++VV I     +G  +  +  + E+
Sbjct: 128 ESFKTSDYVKMLEDVCPEVKTSRPCNINSENLPFLRSVVLI-----RGDKKDYMYTWDEM 182

Query: 196 IARGNAA-DPRLAQVAAGLQATDPINIQFTSGTTGFPKGATLTHRNILNNGFFIGECMKL 254
           + +G+   D  L  V   L   DPINIQ+TSGTTGFPKG  LTH N+LNNG+FIGE MK 
Sbjct: 183 LEKGDEIPDAALCTVQGTLSFDDPINIQYTSGTTGFPKGVVLTHHNLLNNGYFIGEYMKF 242

Query: 255 TPADRLCIPVPLYHCFGMVLGNLACFTHGATIVYPNDGFDPLTVLQTVQDERCTGLHGVP 314
           T  D+LCIPVP YHCFGMVL NLAC THGAT+V P + FDP+  L  ++ E+CT +HGVP
Sbjct: 243 TEKDKLCIPVPFYHCFGMVLSNLACMTHGATMVLPAEHFDPIATLTAIEKEKCTAVHGVP 302

Query: 315 TMFIAELDHPRFAEFNLSTLRTGIMAGSPCPTEVMKRVVEQMNLREITIAYGMTETSPVS 374
           TMFIAEL+HP F++F+LS+LRTGIMAGSPCP E MK+V   MN+R+I I YG TE SP  
Sbjct: 303 TMFIAELEHPLFSKFDLSSLRTGIMAGSPCPIEYMKKVNNLMNMRDIVITYGQTEASPGL 362

Query: 375 CQSSTDTPLSKRVSTVGQVQPHLEVKIVDPDTGAVVPIGQRGEFCTKGYSVMHGYWGDEA 434
             SSTD  L KRVSTVG+  PH E+KI+DP TG +VP G  GE C +GY +M GY+ +  
Sbjct: 363 TMSSTDDSLEKRVSTVGKPMPHTEIKIIDPKTGEIVPRGVPGEICARGYMIMEGYYKNPD 422

Query: 435 KTREAIDEGGWMHTGDLATMDAEGYVNIVGRIKDMVIRGGENIYPREIEEFLYRHPQVQD 494
            T  AIDE GW+HTGDL  +D +GY  I GRIKDMVIRGGENIYPRE+EEFLY HP + D
Sbjct: 423 ATALAIDEKGWLHTGDLGVLDEDGYCKITGRIKDMVIRGGENIYPREVEEFLYTHPMISD 482

Query: 495 VQVVGVPDQKYGEELCAWIIAKPGTQPTEDDIRAFCKGQIAHYKVPRYIRFVTSFPMTVT 554
            QV+GVPD KYGEEL AW+  K G + TE +I+ +CKG+IAHYK+P+YI+FV  FPMTV+
Sbjct: 483 AQVIGVPDLKYGEELMAWVKVKNGCKLTEGEIKEYCKGKIAHYKIPKYIKFVDEFPMTVS 542

Query: 555 GKIQKFKIRDEMKDQLGLEE 574
           GKIQK+K+R+    +LGLEE
Sbjct: 543 GKIQKYKMRETSIKELGLEE 562


Lambda     K      H
   0.320    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 886
Number of extensions: 32
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 578
Length of database: 569
Length adjustment: 36
Effective length of query: 542
Effective length of database: 533
Effective search space:   288886
Effective search space used:   288886
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources