GapMind for catabolism of small carbon sources

 

L-phenylalanine catabolism in Leeuwenhoekiella blandensis MED217

Best path

aroP, PAH, PCBD, QDPR, HPD, hmgA, maiA, fahA, atoA, atoD, atoB

Rules

Overview: Phenylalanine utilization in GapMind is based on MetaCyc pathway L-phenylalanine degradation I (aerobic, via tyrosine, link), pathway II (anaerobic, via phenylacetaldehyde dehydrogenase, link), degradation via phenylpyruvate:ferredoxin oxidoreductase (PMC3346364), or degradation via phenylacetaldehyde:ferredoxin oxidoreductase (PMID:24214948). (MetaCyc describes additional pathways, but they do not result in carbon incorporation or are not reported in prokaryotes, so they are not included in GapMind.)

76 steps (31 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
aroP L-phenylalanine:H+ symporter AroP
PAH phenylalanine 4-monooxygenase MED217_RS03175
PCBD pterin-4-alpha-carbinoalamine dehydratase MED217_RS09520
QDPR 6,7-dihydropteridine reductase MED217_RS06815 MED217_RS13565
HPD 4-hydroxyphenylpyruvate dioxygenase MED217_RS00555
hmgA homogentisate dioxygenase MED217_RS00550
maiA maleylacetoacetate isomerase
fahA fumarylacetoacetate hydrolase MED217_RS11775
atoA acetoacetyl-CoA transferase, A subunit MED217_RS17715
atoD acetoacetyl-CoA transferase, B subunit MED217_RS17705
atoB acetyl-CoA C-acetyltransferase MED217_RS06535 MED217_RS07750
Alternative steps:
aacS acetoacetyl-CoA synthetase
ARO10 phenylpyruvate decarboxylase
ARO8 L-phenylalanine transaminase MED217_RS04810 MED217_RS15130
badH 2-hydroxy-cyclohexanecarboxyl-CoA dehydrogenase MED217_RS08850 MED217_RS02430
badI 2-ketocyclohexanecarboxyl-CoA hydrolase MED217_RS10680 MED217_RS17570
badK cyclohex-1-ene-1-carboxyl-CoA hydratase MED217_RS17570
bamB class II benzoyl-CoA reductase, BamB subunit
bamC class II benzoyl-CoA reductase, BamC subunit
bamD class II benzoyl-CoA reductase, BamD subunit MED217_RS05140
bamE class II benzoyl-CoA reductase, BamE subunit
bamF class II benzoyl-CoA reductase, BamF subunit
bamG class II benzoyl-CoA reductase, BamG subunit
bamH class II benzoyl-CoA reductase, BamH subunit
bamI class II benzoyl-CoA reductase, BamI subunit
bcrA ATP-dependent benzoyl-CoA reductase, alpha subunit
bcrB ATP-dependent benzoyl-CoA reductase, beta subunit
bcrC ATP-dependent benzoyl-CoA reductase, gamma subunit
bcrD ATP-dependent benzoyl-CoA reductase, delta subunit
boxA benzoyl-CoA epoxidase, subunit A
boxB benzoyl-CoA epoxidase, subunit B
boxC 2,3-epoxybenzoyl-CoA dihydrolase
boxD 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase
Ch1CoA cyclohex-1-ene-1-carbonyl-CoA dehydrogenase MED217_RS15750 MED217_RS13915
dch cyclohexa-1,5-diene-1-carboxyl-CoA hydratase MED217_RS17570
ech (S)-3-hydroxybutanoyl-CoA hydro-lyase MED217_RS17570 MED217_RS10680
fadB (S)-3-hydroxybutanoyl-CoA dehydrogenase MED217_RS07760 MED217_RS08105
gcdH glutaryl-CoA dehydrogenase MED217_RS01145 MED217_RS17515
had 6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase
iorA phenylpyruvate:ferredoxin oxidoreductase, IorA subunit
iorAB phenylpyruvate:ferredoxin oxidoreductase, fused IorA/IorB
iorB phenylpyruvate:ferredoxin oxidoreductase, IorB subunit
livF L-phenylalanine ABC transporter, ATPase component 1 (LivF) MED217_RS14395 MED217_RS04680
livG L-phenylalanine ABC transporter, ATPase component 2 (LivG) MED217_RS14395 MED217_RS11085
livH L-phenylalanine ABC transporter, permease component 1 (LivH)
livJ L-phenylalanine ABC transporter, substrate-binding component LivJ/LivK
livM L-phenylalanine ABC transporter, permease component 2 (LivM)
oah 6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase
paaA phenylacetyl-CoA 1,2-epoxidase, subunit A
paaB phenylacetyl-CoA 1,2-epoxidase, subunit B
paaC phenylacetyl-CoA 1,2-epoxidase, subunit C
paaE phenylacetyl-CoA 1,2-epoxidase, subunit E MED217_RS17905
paaF 2,3-dehydroadipyl-CoA hydratase MED217_RS17570
paaG 1,2-epoxyphenylacetyl-CoA isomerase / 2-(oxepinyl)acetyl-CoA isomerase / didehydroadipyl-CoA isomerase MED217_RS17570
paaH 3-hydroxyadipyl-CoA dehydrogenase MED217_RS07760 MED217_RS08105
paaJ1 3-oxo-5,6-dehydrosuberyl-CoA thiolase MED217_RS07750 MED217_RS06535
paaJ2 3-oxoadipyl-CoA thiolase MED217_RS07750 MED217_RS06535
paaK phenylacetate-CoA ligase
paaZ1 oxepin-CoA hydrolase
paaZ2 3-oxo-5,6-didehydrosuberyl-CoA semialdehyde dehydrogenase
pad-dh phenylacetaldehyde dehydrogenase MED217_RS15030 MED217_RS07225
padB phenylacetyl-CoA dehydrogenase, PadB subunit
padC phenylacetyl-CoA dehydrogenase, PadC subunit
padD phenylacetyl-CoA dehydrogenase, PadD subunit
padE phenylglyoxylate dehydrogenase, gamma subunit
padF phenylglyoxylate dehydrogenase, delta subunit
padG phenylglyoxylate dehydrogenase, alpha subunit
padH phenylglyoxylate dehydrogenase, epsilon subunit
padI phenylglyoxylate dehydrogenase, beta subunit
pfor phenylacetaldeyde:ferredoxin oxidoreductase
pimB 3-oxopimeloyl-CoA:CoA acetyltransferase MED217_RS07750 MED217_RS06535
pimC pimeloyl-CoA dehydrogenase, small subunit
pimD pimeloyl-CoA dehydrogenase, large subunit
pimF 6-carboxyhex-2-enoyl-CoA hydratase
PPDCalpha phenylpyruvate decarboxylase, alpha subunit MED217_RS06150
PPDCbeta phenylpyruvate decarboxylase, beta subunit MED217_RS06150 MED217_RS01900

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory