Annotation: NCBI__GCF_000166275.1:WP_040302809.1
Length: 416 amino acids
Source: GCF_000166275.1 in NCBI
Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
---|---|---|---|---|---|---|---|---|---|
L-threonine catabolism | kbl | lo | 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase; AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; Glycine acetyltransferase; EC 2.3.1.29; EC 2.3.1.47 (characterized) | 36% | 99% | 222.2 | serine C-palmitoyltransferase (EC 2.3.1.50) | 34% | 238.8 |
View WP_040302809.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
MDLFAKLKTNMGPLGKHSQFSDGYYMFPKLEGEIAPRMKFQGKEVLTWSLNNYLGLANHP EVRKTDAEAAAKWGAAYPMGARMMSGQTSLHEKLESELAKFVGKEKSYLLNYGYQGIMSV IDALLDRKDVVVYDSECHACIIDALRMHMGKRYVFPHNDIENCEKQLERATKLAQETGGG ILVITEGVFGMTGDQGKLDEICALKEKFEFRLLVDDAHGFGTLGKTGAGTHEEQGVINEV DLYFSTFAKSMASIGAFIAGDEKVIHYLRFNMRSQIFAKSLPMILVEGALKRLELLQTQP ELKDNLWKVVNALRDGLHREGFSTGQSNSPVTPVVLNGTVGEAAALSHDLRENFGIFCSV VIYPVVPKGMIILRLIPTAVHSLEDVEETINAFATVKEKLSGGIYKNSELAISFGE
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory