Alignments for a candidate for mcmA in Algoriphagus machipongonensis PR1

GapMind for catabolism of small carbon sources

Alignments for a candidate for mcmA in Algoriphagus machipongonensis PR1

Align Methylmalonyl-CoA mutase (EC 5.4.99.2) (characterized)
to candidate WP_008202643.1 ALPR1_RS17365 methylmalonyl-CoA mutase

Query= reanno::PS:Dsui_0519
         (721 letters)



>NCBI__GCF_000166275.1:WP_008202643.1
          Length = 706

 Score =  941 bits (2431), Expect = 0.0
 Identities = 460/680 (67%), Positives = 563/680 (82%)

Query: 37  WITPEGLTVKPLYTKKDVEDLPYADTLPGFAPYLRGPQATMYAVRPWTIRQYAGFSTAEE 96
           W T E + V   +  K++ +L +   + G APYLRGP  +MYA+R WTIRQYAGFSTAEE
Sbjct: 24  WETAEKIEVPSEFDPKEIANLKHIHFIAGTAPYLRGPYRSMYAMRAWTIRQYAGFSTAEE 83

Query: 97  SNAFYRKALAAGGQGVSVAFDLATHRGYDSDNPRVLGDVGKAGVAIDSVEDMKILFDGIP 156
           SNAFYRK L AG +G+SVAFDLATHRGYDSD+PRVLGDVGKAGVAIDSVEDMKILFD IP
Sbjct: 84  SNAFYRKNLEAGQKGLSVAFDLATHRGYDSDHPRVLGDVGKAGVAIDSVEDMKILFDQIP 143

Query: 157 LDKISVSMTMNGAVLPILAGYIVAAEEQGVSQEQLSGTIQNDILKEFMVRNTYIYPPKPS 216
           LD++SVSMTMNGAV+PILA YIVAAEEQGV  EQLSGTIQNDILKEFMVRNTYIYPP+PS
Sbjct: 144 LDQMSVSMTMNGAVIPILAFYIVAAEEQGVKPEQLSGTIQNDILKEFMVRNTYIYPPQPS 203

Query: 217 MKIISDIFGYTAQHMPKFNSISISGYHIQEAGANQAIELAFTLADGMEYVRTGIASGLDV 276
           MKII+DIF YT+QH+PKFNSISISGYH+QEAGA   IELA+TLADG EY+RTG+ +G+D+
Sbjct: 204 MKIIADIFEYTSQHIPKFNSISISGYHMQEAGATADIELAYTLADGWEYLRTGVKAGMDI 263

Query: 277 DAFAGRLSFFWAVGMNFYLEIAKMRAGRMLWHRIMSQFNPKSAKSLMLRTHSQTSGWSLT 336
           D FA RLSFFWA+GMN+++EIAKMRAGRMLW +I+ +FNPK+ KSL LRTH QTSGWSLT
Sbjct: 264 DDFAPRLSFFWAIGMNYFMEIAKMRAGRMLWAKIVQKFNPKNPKSLALRTHCQTSGWSLT 323

Query: 337 EQDPYNNVVRTTIEAMAAVFGGTQSLHTNALDEAIALPTEFSARIARNTQLIIQEETHIC 396
           EQDP+NNV RT +EA+AA  GGTQSLHTN+LDEAIALPT+FSARIARNTQL++Q+ET I 
Sbjct: 324 EQDPFNNVTRTAVEALAAAMGGTQSLHTNSLDEAIALPTDFSARIARNTQLVLQDETGIT 383

Query: 397 NVVDPWAGSYMMEKLTQDMADKAWSIIQEIEAMGGMTKAVESGWAKMQVETCAADKQARI 456
           + VDPW GS+ +E LT  +  KAW +I+E+E++GGM KA+ESG  K+++E  AA KQARI
Sbjct: 384 HAVDPWGGSHYVEYLTDQLVQKAWGLIEEVESLGGMAKAIESGVPKLRIEEAAAKKQARI 443

Query: 457 DSGKDVIVGVNKYKLAKEDQIDILDIDNHAVREAQIARLKKIRASRDSAAVQAALDALTQ 516
           DSGKD+IVGVN++K+  E +I++ D+DN  VR++QIARL+ I+  RD  AV  +L ALT 
Sbjct: 444 DSGKDIIVGVNRFKVDGESEIEVRDVDNAEVRKSQIARLQAIKEKRDQQAVDKSLQALTD 503

Query: 517 CAESGEGNLLDLSVKAIRLRATVGEVSDALEKVFGRFRANNQTISGVYGGVVEGQESWES 576
            A SG+GNLL L+V A R RAT+GE+S ALE+VFGR +A+N++I+GVY   VE QES++ 
Sbjct: 504 AANSGKGNLLGLAVSAARNRATLGEISSALEEVFGRHKAHNKSITGVYSKEVENQESFKK 563

Query: 577 IKADIAKFAEEEGRRPRIMIAKLGQDGHDRGAKVVATAFADLGFDIDMGPLFQTPEEAAR 636
             +   +F E EGRRPRIM+AK+GQDGHDRGAKV+AT FADLGFD+D+GPLFQTPEE A 
Sbjct: 564 ALSLSNQFEELEGRRPRIMVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPEEVAE 623

Query: 637 QAVENDVHAIGVSSLAAGHKTLLPALVNSLKEQGADDIIVFAGGVIPAQDYDTLYAAGAK 696
           QA+ENDVH +G SSLAAGHKTL+P L+ +L + G  DI+V AGGVIP +DYD L  AG  
Sbjct: 624 QAIENDVHIVGASSLAAGHKTLIPQLIGALDKLGRPDIMVVAGGVIPTKDYDFLKDAGVA 683

Query: 697 AIFGPGTRIEDSAKRVLEEI 716
            +FGPGT + ++A ++LE++
Sbjct: 684 EVFGPGTVLSEAAIKILEKL 703


Lambda     K      H
   0.317    0.132    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1353
Number of extensions: 46
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 721
Length of database: 706
Length adjustment: 39
Effective length of query: 682
Effective length of database: 667
Effective search space:   454894
Effective search space used:   454894
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources