Alignments for a candidate for prpE in Algoriphagus machipongonensis PR1

GapMind for catabolism of small carbon sources

Alignments for a candidate for prpE in Algoriphagus machipongonensis PR1

Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate WP_008198284.1 ALPR1_RS02860 acetate--CoA ligase

Query= SwissProt::P27550
         (652 letters)



>NCBI__GCF_000166275.1:WP_008198284.1
          Length = 630

 Score =  706 bits (1822), Expect = 0.0
 Identities = 348/621 (56%), Positives = 438/621 (70%), Gaps = 7/621 (1%)

Query: 28  YQQSINVPDTFWGEQGKILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQ 87
           YQ+S+  P+ FW        W K + K    +F   +V  KW+ +G LN+  N  +R+L 
Sbjct: 15  YQKSVAQPEEFWARIADSFHWRKRWDKTLKWNFEGPDV--KWFLNGKLNITENIFERYLF 72

Query: 88  ENGDRTAIIWEGDDASQS-KHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAA 146
             GDR AIIWE +D +++ + ++Y+EL  +V RF+N L   GI KGD V IYMPMVPEAA
Sbjct: 73  TIGDRPAIIWEPNDPNEAGRTLTYRELFEEVSRFSNALKSKGIGKGDKVIIYMPMVPEAA 132

Query: 147 VAMLACARIGAVHSVIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDAL 206
           VAMLACARIGA+HSV+F GFS  A+A RI D  ++ V+TSD   R  + I +K  VD+AL
Sbjct: 133 VAMLACARIGAIHSVVFAGFSSNALADRINDCEAKAVLTSDGNFRGSKKIAVKAVVDEAL 192

Query: 207 KNPNVTSVEHVVVLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSG 266
                +SVE V+V +RT   +  Q+GRD WWHD+V   S    AEEM++ED LFILYTSG
Sbjct: 193 TK---SSVETVIVYQRTHQDVTMQDGRDYWWHDVVADESKDCPAEEMDSEDMLFILYTSG 249

Query: 267 STGKPKGVLHTTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAT 326
           STGKPKGV+HTTGGY+VY+  +F+ VF Y PGD+YWCTADVGW+TGHSY++YGPL  GAT
Sbjct: 250 STGKPKGVVHTTGGYMVYSKYSFENVFQYSPGDVYWCTADVGWITGHSYIVYGPLLAGAT 309

Query: 327 TLMFEGVPNWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSV 386
           T+MFEGVP +P   R   +V+K++VN  YTAPTAIRAL A G   IE  D SSL++LGSV
Sbjct: 310 TIMFEGVPTFPDCGRFWAIVEKYKVNQFYTAPTAIRALQAYGTVEIEKYDLSSLKVLGSV 369

Query: 387 GEPINPEAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQP 446
           GEPIN EAW WY   IG  KCP+VDTWWQTETGG M++P+ G T  K   AT P  GVQ 
Sbjct: 370 GEPINEEAWHWYHTHIGKNKCPIVDTWWQTETGGIMVSPIAGITPTKPAYATLPLPGVQL 429

Query: 447 ALVDNEGNPLEG-ATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRD 505
            +VD EGN L G + EG+L I   WP   RT +GDH+R +QTYFST+K MYF+GDG +RD
Sbjct: 430 CIVDPEGNELTGNSVEGNLCIKFPWPSMIRTTYGDHDRCKQTYFSTYKGMYFTGDGVKRD 489

Query: 506 EDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTL 565
            DGYY I GRVDDV+NVSGHR+GTAE+E+A+  HPK+ E+AVVG PH++KGQ IYAYV  
Sbjct: 490 HDGYYRILGRVDDVINVSGHRMGTAEVENAINEHPKVIESAVVGYPHDVKGQGIYAYVIC 549

Query: 566 NHGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTSN 625
           +        L  E++  V K IGP+A PD +     LPKTRSGKIMRRILRK+A  +  N
Sbjct: 550 DLTNRTEDNLVNEIKEMVSKIIGPIAKPDKIQLVPGLPKTRSGKIMRRILRKVAENNLDN 609

Query: 626 LGDTSTLADPGVVEKLLEEKQ 646
           +GDTSTL DP VVEK+++ ++
Sbjct: 610 MGDTSTLLDPDVVEKIIDGRK 630


Lambda     K      H
   0.317    0.135    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1248
Number of extensions: 61
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 652
Length of database: 630
Length adjustment: 38
Effective length of query: 614
Effective length of database: 592
Effective search space:   363488
Effective search space used:   363488
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources