Alignments for a candidate for lpd in Algoriphagus machipongonensis PR1

GapMind for catabolism of small carbon sources

Alignments for a candidate for lpd in Algoriphagus machipongonensis PR1

Align dihydrolipoyl dehydrogenase (EC 1.8.1.4) (characterized)
to candidate WP_008202812.1 ALPR1_RS17920 dihydrolipoyl dehydrogenase

Query= BRENDA::P50970
         (466 letters)



>NCBI__GCF_000166275.1:WP_008202812.1
          Length = 465

 Score =  449 bits (1156), Expect = e-131
 Identities = 236/461 (51%), Positives = 316/461 (68%), Gaps = 2/461 (0%)

Query: 5   FDLIVLGGGPGGYVAAIRAAQLNLKVALVERVHLGGICLNWGCIPTKSLLRSAEVYHEMQ 64
           FDLIV+G GPGGYVAAIRA+QL LK A+VE   LGGICLNWGCIPTK+LL+SA+V+  + 
Sbjct: 6   FDLIVVGSGPGGYVAAIRASQLGLKTAVVEAAELGGICLNWGCIPTKALLKSAQVFEYIN 65

Query: 65  NAEAYGLTSFKPDFDLDKIIARSREVATRLASGVKTLLRKNKVEVISGVGQLTGNQQMLV 124
           +A  YG+T      D D I+ RSR VA  ++ GV  L++KNK+EVI G G++   +++ V
Sbjct: 66  HAADYGITVGDAKADFDGIVKRSRGVADGMSKGVTFLMKKNKIEVIKGWGKIQPGKKVEV 125

Query: 125 ETTEGEEKILEAKDIIIATGARARQLPNVHSDGKHIWTYHHALKPPAMPKKLLVIGSGAI 184
             +E ++ +  A +IIIATGAR+R+LP++  D K I  Y  A+    MPKK++V+GSGAI
Sbjct: 126 TDSEEKKTVYSADNIIIATGARSRELPSMKIDKKKIIGYREAMTLDKMPKKMVVVGSGAI 185

Query: 185 GIEFASFYADFGAEVSIVEHAPQILPMEDAEVSAYVAKAFKKRGIRILTQSALQNLTPDD 244
           GIEFA FYA  GAEV+IVE+  +I+P+EDAEVS  + K +KK G+ I+T S +  +    
Sbjct: 186 GIEFAYFYATMGAEVTIVEYLDRIVPVEDAEVSKTLEKIYKKAGMTIMTSSEVTAVDTKG 245

Query: 245 EGVTAEIAGADGKVTKERFSHAIVAIGVVANVENIGLDKLGIKLDRGFIAVDGFGRTNVD 304
            G    +  A G+ T E     + A GVV+N+EN GL+ +GI +D+G I VD + +TN+ 
Sbjct: 246 SGCKVTVKTAKGEETLE-CDIVLSAAGVVSNLENCGLEDVGILVDKGKIKVDEYYKTNMP 304

Query: 305 HVWAIGDVAGAPCLAHKASHQGVIAAEAIAGCDHVHPLNTQNIPGCTYARPQVASVGLTE 364
             +AIGDV   P LAH AS +G+I  E IAG  +  PL+  NIPGCTY  P++ASVG+TE
Sbjct: 305 GYYAIGDVIPGPALAHVASAEGIICVEKIAG-QNPEPLDYGNIPGCTYCSPEIASVGMTE 363

Query: 365 EKARQQGYNVKIGNFPFIANGKAIAQGATDGFVKTVFDADSGALLGAHMVGAEVTEMIQG 424
            KA++ GY +++G FPF A+GKA A GA DGFVK VFD   G LLGAHM+G  VTEMI  
Sbjct: 364 AKAKEAGYELRVGKFPFSASGKASAAGAKDGFVKLVFDKKYGELLGAHMIGFNVTEMIAE 423

Query: 425 YTVARTLETTEAEIMETIFPHPTLSEAMHESVLAAYGRALH 465
               R LETT  E+++T+ PHPT+SEA+ E+  AAY   +H
Sbjct: 424 IVAIRKLETTGHELIKTVHPHPTMSEAVMEAAAAAYDEVIH 464


Lambda     K      H
   0.319    0.135    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 592
Number of extensions: 24
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 466
Length of database: 465
Length adjustment: 33
Effective length of query: 433
Effective length of database: 432
Effective search space:   187056
Effective search space used:   187056
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

Align candidate WP_008202812.1 ALPR1_RS17920 (dihydrolipoyl dehydrogenase)
to HMM TIGR01350 (lpdA: dihydrolipoyl dehydrogenase (EC 1.8.1.4))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01350.hmm
# target sequence database:        /tmp/gapView.3584537.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01350  [M=461]
Accession:   TIGR01350
Description: lipoamide_DH: dihydrolipoyl dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   2.2e-177  576.3  13.8   2.5e-177  576.1  13.8    1.0  1  NCBI__GCF_000166275.1:WP_008202812.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000166275.1:WP_008202812.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  576.1  13.8  2.5e-177  2.5e-177       1     460 [.       5     464 ..       5     465 .] 0.97

  Alignments for each domain:
  == domain 1  score: 576.1 bits;  conditional E-value: 2.5e-177
                             TIGR01350   1 eydvvviGgGpgGYvaAiraaqlglkvalvekeklGGtClnvGCiPtKalLksaevveelkeakelgievenv 73 
                                           ++d++v+G+GpgGYvaAira+qlglk+a+ve  +lGG+Cln+GCiPtKalLksa+v+e++++a+++gi+v + 
  NCBI__GCF_000166275.1:WP_008202812.1   5 KFDLIVVGSGPGGYVAAIRASQLGLKTAVVEAAELGGICLNWGCIPTKALLKSAQVFEYINHAADYGITVGDA 77 
                                           59*********************************************************************** PP

                             TIGR01350  74 kldlekllerkekvvkklvgGvkaLlkknkvevikGeaklldkkevevkkekke.kkleakniiiAtGsepre 145
                                           k d++ +++r++ v + +++Gv++L+kknk+evikG +k++  k+vev++++++ ++++a+niiiAtG++ re
  NCBI__GCF_000166275.1:WP_008202812.1  78 KADFDGIVKRSRGVADGMSKGVTFLMKKNKIEVIKGWGKIQPGKKVEVTDSEEKkTVYSADNIIIATGARSRE 150
                                           *************************************************9998746778************** PP

                             TIGR01350 146 lplkleedekvvitseealelkevpeslvivGgGviGvEfasifaklGvkvtvielldrilpaldaevskvlk 218
                                           lp  ++ d+k +i ++ea++l+++p+++v+vG+G+iG+Efa+++a++G++vt++e+ldri+p++daevsk+l+
  NCBI__GCF_000166275.1:WP_008202812.1 151 LPS-MKIDKKKIIGYREAMTLDKMPKKMVVVGSGAIGIEFAYFYATMGAEVTIVEYLDRIVPVEDAEVSKTLE 222
                                           ***.9******************************************************************** PP

                             TIGR01350 219 kklkkkgvkiltnakvtevekeedevvveakkk.evetleaekvLvavGrkpnleelgleklgveldergaik 290
                                           k +kk g++i+t+++vt+v ++    +v+ k    +etle + vL a G+ +nle+ gle +g+ +d+ g+ik
  NCBI__GCF_000166275.1:WP_008202812.1 223 KIYKKAGMTIMTSSEVTAVDTKGSGCKVTVKTAkGEETLECDIVLSAAGVVSNLENCGLEDVGILVDK-GKIK 294
                                           *****************99888877665444443469******************************9.99** PP

                             TIGR01350 291 vdeelrtnvpgiyaiGDvigklmLAhvAskegvvaaekiagkekseidykavPsviytePevasvGlteeqak 363
                                           vde+++tn+pg yaiGDvi ++ LAhvAs+eg++++ekiag++++ +dy  +P ++y++Pe+asvG+te++ak
  NCBI__GCF_000166275.1:WP_008202812.1 295 VDEYYKTNMPGYYAIGDVIPGPALAHVASAEGIICVEKIAGQNPEPLDYGNIPGCTYCSPEIASVGMTEAKAK 367
                                           ************************************************************************* PP

                             TIGR01350 364 eegievkvgkfpfaangkalaleetdGfvkvivdkktgeilGahivgaeaseliselalaveleltveelakt 436
                                           e+g+e++vgkfpf+a+gka a++ +dGfvk+++dkk+ge+lGah++g +++e+i+e++++ +le+t +el kt
  NCBI__GCF_000166275.1:WP_008202812.1 368 EAGYELRVGKFPFSASGKASAAGAKDGFVKLVFDKKYGELLGAHMIGFNVTEMIAEIVAIRKLETTGHELIKT 440
                                           ************************************************************************* PP

                             TIGR01350 437 ihpHPtlsEaikeaalaalgkaih 460
                                           +hpHPt+sEa++eaa+aa+++ ih
  NCBI__GCF_000166275.1:WP_008202812.1 441 VHPHPTMSEAVMEAAAAAYDEVIH 464
                                           *********************999 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (461 nodes)
Target sequences:                          1  (465 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 16.30
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources