GapMind for catabolism of small carbon sources

 

Protein WP_013404046.1 in Caldicellulosiruptor hydrothermalis 108

Annotation: NCBI__GCF_000166355.1:WP_013404046.1

Length: 276 amino acids

Source: GCF_000166355.1 in NCBI

Candidate for 9 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-maltose catabolism musG lo ABC-type transporter, permease components, component of Maltose transporter, MusEFGKI (characterized) 32% 91% 147.1 Putative sugar-binding integral membrane transport protein, component of α-glucoside uptake permease, Agl3E/Agl3F/Agl3G. Plays a role in normal morphogenesis and antibiotic production. Strongly induced by trehalose and melibiose, and weakly induced by lactose and glycerol but not glucose (Hillerich and Westpheling 2006).The operon is controlled by a GntR homologue, Agl3R, and downstream of the gntR 32% 159.8
D-cellobiose catabolism SMc04257 lo ABC transporter for D-Cellobiose and D-Salicin, permease component 1 (characterized) 31% 91% 128.3 Putative sugar-binding integral membrane transport protein, component of α-glucoside uptake permease, Agl3E/Agl3F/Agl3G. Plays a role in normal morphogenesis and antibiotic production. Strongly induced by trehalose and melibiose, and weakly induced by lactose and glycerol but not glucose (Hillerich and Westpheling 2006).The operon is controlled by a GntR homologue, Agl3R, and downstream of the gntR 32% 159.8
D-cellobiose catabolism gtsC lo ABC transporter for D-Galactose and D-Glucose, permease component 2 (characterized) 30% 99% 120.9 Putative sugar-binding integral membrane transport protein, component of α-glucoside uptake permease, Agl3E/Agl3F/Agl3G. Plays a role in normal morphogenesis and antibiotic production. Strongly induced by trehalose and melibiose, and weakly induced by lactose and glycerol but not glucose (Hillerich and Westpheling 2006).The operon is controlled by a GntR homologue, Agl3R, and downstream of the gntR 32% 159.8
D-galactose catabolism PfGW456L13_1896 lo ABC transporter for D-Galactose and D-Glucose, permease component 2 (characterized) 30% 99% 120.9 Putative sugar-binding integral membrane transport protein, component of α-glucoside uptake permease, Agl3E/Agl3F/Agl3G. Plays a role in normal morphogenesis and antibiotic production. Strongly induced by trehalose and melibiose, and weakly induced by lactose and glycerol but not glucose (Hillerich and Westpheling 2006).The operon is controlled by a GntR homologue, Agl3R, and downstream of the gntR 32% 159.8
D-glucose catabolism gtsC lo ABC transporter for D-Galactose and D-Glucose, permease component 2 (characterized) 30% 99% 120.9 Putative sugar-binding integral membrane transport protein, component of α-glucoside uptake permease, Agl3E/Agl3F/Agl3G. Plays a role in normal morphogenesis and antibiotic production. Strongly induced by trehalose and melibiose, and weakly induced by lactose and glycerol but not glucose (Hillerich and Westpheling 2006).The operon is controlled by a GntR homologue, Agl3R, and downstream of the gntR 32% 159.8
lactose catabolism gtsC lo ABC transporter for D-Galactose and D-Glucose, permease component 2 (characterized) 30% 99% 120.9 Putative sugar-binding integral membrane transport protein, component of α-glucoside uptake permease, Agl3E/Agl3F/Agl3G. Plays a role in normal morphogenesis and antibiotic production. Strongly induced by trehalose and melibiose, and weakly induced by lactose and glycerol but not glucose (Hillerich and Westpheling 2006).The operon is controlled by a GntR homologue, Agl3R, and downstream of the gntR 32% 159.8
D-maltose catabolism gtsC lo ABC transporter for D-Galactose and D-Glucose, permease component 2 (characterized) 30% 99% 120.9 Putative sugar-binding integral membrane transport protein, component of α-glucoside uptake permease, Agl3E/Agl3F/Agl3G. Plays a role in normal morphogenesis and antibiotic production. Strongly induced by trehalose and melibiose, and weakly induced by lactose and glycerol but not glucose (Hillerich and Westpheling 2006).The operon is controlled by a GntR homologue, Agl3R, and downstream of the gntR 32% 159.8
sucrose catabolism gtsC lo ABC transporter for D-Galactose and D-Glucose, permease component 2 (characterized) 30% 99% 120.9 Putative sugar-binding integral membrane transport protein, component of α-glucoside uptake permease, Agl3E/Agl3F/Agl3G. Plays a role in normal morphogenesis and antibiotic production. Strongly induced by trehalose and melibiose, and weakly induced by lactose and glycerol but not glucose (Hillerich and Westpheling 2006).The operon is controlled by a GntR homologue, Agl3R, and downstream of the gntR 32% 159.8
trehalose catabolism gtsC lo ABC transporter for D-Galactose and D-Glucose, permease component 2 (characterized) 30% 99% 120.9 Putative sugar-binding integral membrane transport protein, component of α-glucoside uptake permease, Agl3E/Agl3F/Agl3G. Plays a role in normal morphogenesis and antibiotic production. Strongly induced by trehalose and melibiose, and weakly induced by lactose and glycerol but not glucose (Hillerich and Westpheling 2006).The operon is controlled by a GntR homologue, Agl3R, and downstream of the gntR 32% 159.8

Sequence Analysis Tools

View WP_013404046.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MGKGIKKFVIELFAIVVCLVIYGIPFFFAIINSLKSRTEAAELSISLPKKLHFLQNYKEV
LTTENYIVLRAFLNSSIITVVSVLILIFVASMTGYILQRRKEEISSIVNSLILMGLMIPP
SVVPTIWILKTLGLYKTLTGMILIEVALSYPFSTLLYTNFMNTIPREIDESAIIDGCGAI
QMFWKIIFPLLKPVTVTVTILNAVHIFNDFVNPLYFLPGARNATVQLTLYNFVSRYSTSW
NLVFADAVLISIPPLIFFIFFNKKIVAGMTAGAIKA

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory