GapMind for catabolism of small carbon sources

 

Alignments for a candidate for braG in Caldicellulosiruptor hydrothermalis 108

Align ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale)
to candidate WP_013403237.1 CALHY_RS06835 ABC transporter ATP-binding protein

Query= uniprot:Q1MCU3
         (247 letters)



>NCBI__GCF_000166355.1:WP_013403237.1
          Length = 256

 Score =  125 bits (315), Expect = 6e-34
 Identities = 81/255 (31%), Positives = 132/255 (51%), Gaps = 24/255 (9%)

Query: 11  LLQVNGVETYYGNIRALAGVDVHVNKGEIVSLIGANGAGKSTLMMTICGSPQARTGSVVF 70
           +L++  V   +G I AL  V++ + KG I+ LIG NGAGK+T+   I G     TG + F
Sbjct: 1   MLRIKNVTVNFGGIVALNNVNIDIEKGAIIGLIGPNGAGKTTVFNVISGIYNPNTGRIEF 60

Query: 71  EGRDITRMPTHEIARLRIAQSPEGRRIFPRMTVLENLQMG----------AGLDNLKHF- 119
              DIT   T++++ L I+++ +  R+F  ++V++N+++             +     F 
Sbjct: 61  SNYDITYKKTYQVSALGISRTFQNIRLFKELSVIDNVKISFHKNISYNLFDAIFRTSKFL 120

Query: 120 ---------AEDVEKIFTLFPRLKERHAQRGGTLSGGEQQMLSIGRALMARPKLLLLDEP 170
                    AE++ KIF L+    E+  +    L  GEQ+ L I RAL   PKLLLLDEP
Sbjct: 121 KEEEQNHKKAEELLKIFGLY----EKRFELAKNLPYGEQRKLEIVRALATSPKLLLLDEP 176

Query: 171 SLGLAPLIVKGIFEAIRKLNEAEGLTVFLVEQNAFAALRLSHRAYVMVNGKVTMSGSGKE 230
           + G+ P   + + + I+ + E   LT+ L+E +    + +  + YV+  G+V   G+  E
Sbjct: 177 AAGMNPQETQELKKLIKFIKERFDLTILLIEHDMSVVMDICEKIYVLDYGEVIAVGTPVE 236

Query: 231 LLANPEVRAAYLEGG 245
           +  NP V  AYL  G
Sbjct: 237 VKNNPRVIEAYLGEG 251


Lambda     K      H
   0.320    0.137    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 144
Number of extensions: 5
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 247
Length of database: 256
Length adjustment: 24
Effective length of query: 223
Effective length of database: 232
Effective search space:    51736
Effective search space used:    51736
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory