D-maltose catabolism in Caldicellulosiruptor hydrothermalis 108

GapMind for catabolism of small carbon sources

D-maltose catabolism in Caldicellulosiruptor hydrothermalis 108

Best path

malE_Aa, malF_Aa, malG_Aa, malK_Aa, malP, pgmB, glk

Rules

Overview: Maltose utilization in GapMind is based on the MetaCyc pathway via maltose phosphorylase (link), or a phosphotransferase system followed by 6-phospho-alphaglucosidase, or a phosphotransferase system followed by a phosphatase back to maltose followed by maltose phosphorylase, or hydrolysis by alpha-glucosidase after uptake, or periplasmic hydrolysis by alpha-glucosidase followed by glucose utilization.

all:

maltose-transport, malP, pgmB and glk
or maltose-PTS, malA and glk
or maltose-PTS, mapP, malP, pgmB and glk
or maltose-transport, susB and glk
or susB and glucose-utilization
Comment: In the phosphorylase pathway, malP forms beta-glucose-1-phosphate and glucose, and beta-phosphoglucomutase forms glucose-6-phosphate, a central metabolite; glucokinase (glk) is also needed to feed the released glucose into central metabolism. Alternatively, PTS systems produce maltose 6'-phosphate, which can be cleaved by 6-phospho-alpha-glucosidase to glucose and glucose-6-phosphate. Alternatively, maltose can be hydrolyzed by alpha-glucosidase (EC 3.2.1.20) in the cytoplasm. Alternatively, maltose can be hydrolyzed by alpha-glucosidase (EC 3.2.1.20) in the periplasm, as in Bacteroides thetaiotaomicron, followed by glucose utilization.

glucose-utilization:

glucose-transport and glk
or glucose-PTS
or gdh, gnl, gadh1, gadh2, gadh3, kguT, kguK, kguD, edd and eda
Comment: Glucose can be taken up and then phosphorylated to glucose 6-phosphate by the kinase glk. Or, both uptake and phosphorylation are catalyzed by a PTS system. Or, glucose is oxidized to glucono-1,5-lactone in the periplasm (by gdh), hydrolyzed to gluconate (by gnl), oxidized to 2-ketogluconate (by gadh123), taken up by kguT, phosphorylated to 2-dehydro-6-phosphogluconate (by kguK), reduced to gluconate 6-phosphate (by kguD), dehydrated by edd to 2-dehydro-3-deoxy-gluconate 6-phosphate, and cleaved by aldolase eda to pyruvate and D-glyceraldehyde 3-phosphate.

glucose-PTS:

ptsG-crr
or bglF
or ptsG and crr
or manX, manY and manZ

glucose-transport:

MFS-glucose
or SSS-glucose
or glcU'
or PAST-A
or SemiSWEET
or SWEET1
or mglA, mglB and mglC
or gtsA, gtsB, gtsC and gtsD
or glcS, glcT, glcU and glcV
or aglE', aglF', aglG' and aglK'
Comment: Transporters and PTS systems were analyzed uzing query: transporter:glucose:D-glucose:D-glucopyranose:ALPHA-GLUCOSE:GLC:CPD-15374

maltose-PTS:

malEIICBA
or malEIICB and malEIIA
Comment: PTS systems form maltose 6'-phosphate

maltose-transport:

malE, malF, malG and malK
or thuE, thuF, thuG and thuK
or malE1, malF1, malG1 and malK1
or malX_Sm, malF_Sm, malG_Sm and malK_Sm
or musE, musF, musG, musK and musI
or malE_Aa, malF_Aa, malG_Aa and malK_Aa
or aglE, aglF, aglG and aglK
or malEF_Bb, malG_Bb and malK_Bb
or malE_Ss, malF_Ss, malG_Ss and malK_Ss
or MAL11
or SUC2
or SUT1
or cscB
or malAP
or malI
Comment: Transporters and PTS systems identified using: query: transporter:maltose:ALPHA-MALTOSE:CPD-15717

89 steps (45 with candidates)

Or see definitions of steps

Step	Description	Best candidate	2nd candidate
malE_Aa	maltose ABC transporter, substrate-binding component	CALHY_RS01380
malF_Aa	maltose ABC transporter, permease component 1	CALHY_RS01360	CALHY_RS02270
malG_Aa	maltose ABC transporter, permease component 2	CALHY_RS01365	CALHY_RS11380
malK_Aa	maltose ABC transporter, ATPase component	CALHY_RS04750	CALHY_RS07865
malP	maltose phosphorylase	CALHY_RS00350	CALHY_RS11370
pgmB	beta-phosphoglucomutase	CALHY_RS11875	CALHY_RS00360
glk	glucokinase	CALHY_RS10640	CALHY_RS10325
Alternative steps:
aglE	maltose ABC transporter, substrate-binding component AglK
aglE'	glucose ABC transporter, substrate-binding component (AglE)
aglF	maltose ABC transporter, permease component 1 (AglF)	CALHY_RS10335	CALHY_RS02270
aglF'	glucose ABC transporter, permease component 1 (AglF)	CALHY_RS10335	CALHY_RS02270
aglG	maltose ABC transporter, permease component 2 (AglG)	CALHY_RS00330	CALHY_RS00400
aglG'	glucose ABC transporter, permease component 2 (AglG)	CALHY_RS00330	CALHY_RS02275
aglK	maltose ABC transporter, ATPase component AglK	CALHY_RS04750	CALHY_RS04225
aglK'	glucose ABC transporter, ATPase component (AglK)	CALHY_RS04750	CALHY_RS04225
bglF	glucose PTS, enzyme II (BCA components, BglF)
crr	glucose PTS, enzyme IIA
cscB	maltose permease
eda	2-keto-3-deoxygluconate 6-phosphate aldolase	CALHY_RS00655	CALHY_RS11140
edd	phosphogluconate dehydratase	CALHY_RS10090
gadh1	gluconate 2-dehydrogenase flavoprotein subunit
gadh2	gluconate 2-dehydrogenase cytochrome c subunit
gadh3	gluconate 2-dehydrogenase subunit 3
gdh	quinoprotein glucose dehydrogenase
glcS	glucose ABC transporter, substrate-binding component (GlcS)
glcT	glucose ABC transporter, permease component 1 (GlcT)
glcU	glucose ABC transporter, permease component 2 (GlcU)	CALHY_RS00330	CALHY_RS03560
glcU'	Glucose uptake protein GlcU
glcV	glucose ABC transporter, ATPase component (GclV)	CALHY_RS04750	CALHY_RS07950
gnl	gluconolactonase
gtsA	glucose ABC transporter, substrate-binding component (GtsA)
gtsB	glucose ABC transporter, permease component 1 (GtsB)	CALHY_RS00460	CALHY_RS11070
gtsC	glucose ABC transporter, permease component 2 (GtsC)	CALHY_RS10330	CALHY_RS00465
gtsD	glucose ABC transporter, ATPase component (GtsD)	CALHY_RS04750	CALHY_RS07865
kguD	2-keto-6-phosphogluconate reductase	CALHY_RS00645	CALHY_RS02550
kguK	2-ketogluconokinase	CALHY_RS02545
kguT	2-ketogluconate transporter
MAL11	maltose permease
malA	6-phospho-alphaglucosidase	CALHY_RS03990
malAP	maltose permease
malE	maltose ABC transporter, substrate-binding component MalE	CALHY_RS01380
malE1	maltose ABC transporter, substrate-binding component (MalE1/MalE2)	CALHY_RS01380
malE_Ss	maltose ABC transporter, substrate-binding component
malEF_Bb	maltose ABC transporter, fused substrate-binding and permease component 1
malEIIA	maltose phosphotransferase system, EII-A component (PtsG/YpqE/GamP)
malEIICB	maltose phosphotransferase system, EII-CB components curated:SwissProt::P54715
malEIICBA	maltose phosphotransferase system, EII-CBA components
malF	maltose ABC transporter, permease component 1 (MalF)
malF1	maltose ABC transporter, permease component 1 (MalF1)
malF_Sm	maltose ABC transporter, permease component 1	CALHY_RS01360
malF_Ss	maltose ABC transporter, permease component 1
malG	maltose ABC transporter, permease component 2 (MalG)	CALHY_RS01365	CALHY_RS11380
malG1	maltose ABC transporter, permease component 2 (MalG1/MalG2)
malG_Bb	maltose ABC transporter, permease component 2	CALHY_RS01365	CALHY_RS11380
malG_Sm	maltose ABC transporter, permease component 2	CALHY_RS01365	CALHY_RS02510
malG_Ss	maltose ABC transporter, permease component 2
malI	maltose transporter
malK	maltose ABC transporter, ATPase component MalK	CALHY_RS04750	CALHY_RS07865
malK1	maltose ABC transporter, ATPase component	CALHY_RS04750	CALHY_RS07865
malK_Bb	maltose ABC transporter, ATPase component	CALHY_RS04750	CALHY_RS07865
malK_Sm	maltose ABC transporter, ATPase component	CALHY_RS04750	CALHY_RS07865
malK_Ss	maltose ABC transporter, ATPase component	CALHY_RS12040	CALHY_RS11485
malX_Sm	maltose ABC transporter, substrate-binding component
manX	glucose PTS, enzyme EIIAB
manY	glucose PTS, enzyme EIIC
manZ	glucose PTS, enzyme EIID
mapP	maltose 6'-phosphate phosphatase
MFS-glucose	glucose transporter, MFS superfamily
mglA	glucose ABC transporter, ATP-binding component (MglA)	CALHY_RS00570	CALHY_RS00530
mglB	glucose ABC transporter, substrate-binding component	CALHY_RS00565	CALHY_RS00525
mglC	glucose ABC transporter, permease component (MglC)	CALHY_RS00575	CALHY_RS00535
musE	maltose ABC transporter, substrate-binding component MusE
musF	maltose ABC transporter, permease component 1 (MusF)	CALHY_RS11070
musG	maltose ABC transporter, permease component 2 (MusG)	CALHY_RS11065	CALHY_RS00330
musI	maltose ABC transporter, uncharacterized membrane component MusI
musK	maltose ABC transporter, ATPase component MusK	CALHY_RS04750	CALHY_RS07950
PAST-A	proton-associated sugar transporter A
ptsG	glucose PTS, enzyme IICB
ptsG-crr	glucose PTS, enzyme II (CBA components, PtsG)
SemiSWEET	Sugar transporter SemiSWEET
SSS-glucose	Sodium/glucose cotransporter
SUC2	maltose:H+ symporter
susB	alpha-glucosidase (maltase)	CALHY_RS12470	CALHY_RS00860
SUT1	maltose:H+ symporter
SWEET1	bidirectional sugar transporter SWEET1
thuE	maltose ABC transporter, substrate-binding component ThuE	CALHY_RS02265
thuF	maltose ABC transporter, permease component 1 (ThuF)	CALHY_RS02270	CALHY_RS11120
thuG	maltose ABC transporter, permease component 2 (ThuG)	CALHY_RS02275	CALHY_RS10330
thuK	maltose ABC transporter, ATPase component ThuK	CALHY_RS04750	CALHY_RS07865

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources