catabolism of small carbon sources in Bacillus coahuilensis m4-4

GapMind for catabolism of small carbon sources

catabolism of small carbon sources in Bacillus coahuilensis m4-4

Pathways are sorted by name. Sort by completeness instead.

Pathway	Steps
acetate	satP, ackA, pta
D-alanine	cycA, dadA
alanine	alsT
arabinose	gguA, gguB, chvE, araA, araB, araD
arginine	bgtB, artP, rocF, rocD, PRO3, put1, putA
asparagine	agcS, ans
aspartate	glt
cellobiose	bgl, ptsG-crr
citrate	SLC13A5, acn, icd
citrulline	AO353_03055, AO353_03050, AO353_03045, AO353_03040, citrullinase, rocD, PRO3, put1, putA
deoxyinosine	nupC, deoD, deoB, deoC, adh, ackA, pta
deoxyribonate	deoxyribonate-transport, deoxyribonate-dehyd, ketodeoxyribonate-cleavage, garK, atoA, atoD, atoB
deoxyribose	deoP, deoK, deoC, adh, ackA, pta
ethanol	etoh-dh-nad, adh, ackA, pta
fructose	Slc2a5, scrK
fucose	fucP, fucU, fucI, fucK, fucA, tpi, aldA
fumarate	Dshi_1194, Dshi_1195
galactose	gguA, gguB, chvE, galK, galT, galE, pgmA
galacturonate	exuT, udh, gli, gci, kdgD, dopDH
gluconate	gntT, gntK, gnd
glucose	ptsG-crr
glucose-6-P	uhpT
glucosamine	gamP, nagB
glucuronate	exuT, udh, gci, kdgD, dopDH
glutamate	gltS, gdhA
glycerol	glpF, glpK, glpD, tpi
histidine	PA5503, PA5504, PA5505, hutH, hutU, hutI, hutG
isoleucine	brnQ, ofo, acdH, ech, ivdG, fadA, pccA, pccB, epi, mcmA
4-hydroxybenzoate	pcaK, pobA, praA, praB, praC, praD, mhpD, mhpE, adh, ackA, pta
D-lactate	lctP, D-LDH
L-lactate	lctP, L-LDH
lactose	lacP, lacZ, galK, galT, galE, pgmA, glk
leucine	brnQ, ilvE, ofo, liuA, liuB, liuD, liuC, liuE, atoA, atoD, atoB
lysine	lysP, davB, davA, davT, davD, gcdG, gcdH, ech, fadB, atoB
L-malate	Dshi_1194, Dshi_1195
maltose	susB, ptsG-crr
mannitol	PLT5, mt2d, scrK
mannose	STP6, man-isomerase, scrK
myoinositol	iolT, iolG, iolE, iolD, iolB, iolC, iolJ, mmsA, tpi
NAG	nagEIIA, nagPcb, nagA, nagB
2-oxoglutarate	Psest_0084, Psest_0085
phenylacetate	paaT, paaK, paaA, paaB, paaC, paaE, paaG, paaZ1, paaZ2, paaJ1, paaF, paaH, paaJ2
phenylalanine	aroP, ARO8, iorAB, paaA, paaB, paaC, paaE, paaG, paaZ1, paaZ2, paaJ1, paaF, paaH, paaJ2
proline	putP, put1, putA
propionate	lctP, prpE, pccA, pccB, epi, mcmA
putrescine	potA, potB, potC, potD, patA, patD, gabT, gabD
pyruvate	SLC5A8
rhamnose	rhaT, rhaM, rhaA, rhaB, rhaD, tpi, aldA
ribose	rbsU, rbsK
D-serine	cycA, dsdA
serine	serP, sdaB
sorbitol	SOT, sdh, scrK
succinate	satP
sucrose	ams, ptsG-crr
threonine	tdcC, tdh, tynA, yvgN, aldA, L-LDH
thymidine	nupC, deoA, deoB, deoC, adh, ackA, pta
trehalose	treEIIA, treB, treC, glk
tryptophan	tnaT, tnaA
tyrosine	aroP, HPD, hmgA, maiA, fahA, atoA, atoD, atoB
valine	brnQ, ofo, acdH, ech, bch, mmsB, mmsA, pccA, pccB, epi, mcmA
xylitol	fruI, x5p-reductase
xylose	xylF, xylG, xylH, xylA, xylB

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources