GapMind for catabolism of small carbon sources

 

Protein WP_014266682.1 in Granulicella mallensis MP5ACTX8

Annotation: NCBI__GCF_000178955.2:WP_014266682.1

Length: 353 amino acids

Source: GCF_000178955.2 in NCBI

Candidate for 7 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-sorbitol (glucitol) catabolism sdh med L-iditol 2-dehydrogenase; EC 1.1.1.14 (characterized) 33% 97% 197.6 L-idonate 5-dehydrogenase (EC 1.1.1.264); L-idonate 5-dehydrogenase (NAD+) (EC 1.1.1.366) 35% 188.7
xylitol catabolism xdhA med L-iditol 2-dehydrogenase; EC 1.1.1.14 (characterized) 33% 97% 197.6 L-idonate 5-dehydrogenase (EC 1.1.1.264); L-idonate 5-dehydrogenase (NAD+) (EC 1.1.1.366) 35% 188.7
D-xylose catabolism xdhA med L-iditol 2-dehydrogenase; EC 1.1.1.14 (characterized) 33% 97% 197.6 L-idonate 5-dehydrogenase (EC 1.1.1.264); L-idonate 5-dehydrogenase (NAD+) (EC 1.1.1.366) 35% 188.7
D-mannitol catabolism mt2d lo mannitol 2-dehydrogenase (EC 1.1.1.67) (characterized) 33% 91% 183.7 L-iditol 2-dehydrogenase; EC 1.1.1.14 33% 197.6
L-threonine catabolism tdh lo L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103 (uncharacterized) 35% 100% 174.9 L-iditol 2-dehydrogenase; EC 1.1.1.14 33% 197.6
L-rhamnose catabolism LRA5 lo 2-dehydro-3-deoxy-L-rhamnonate dehydrogenase (NAD(+)); 2-keto-3-deoxy-L-rhamnonate dehydrogenase; KDRDH; L-KDR dehydrogenase; EC 1.1.1.401 (characterized) 31% 99% 167.9 L-iditol 2-dehydrogenase; EC 1.1.1.14 33% 197.6
xylitol catabolism x5p-reductase lo Lmo2663 protein (characterized, see rationale) 31% 99% 167.5 L-iditol 2-dehydrogenase; EC 1.1.1.14 33% 197.6

Sequence Analysis Tools

View WP_014266682.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MKALLLSEYNHLAIQDLPDPTPGPDELLIQVAACGICGSDVHGYDGSTGRRIPPIVMGHE
AAGIVAAVGTEVSGFAPGDRVTFDSTVFCGVCDHCLQGQVNLCDNRQVIGVSCGEYRRAG
AFAELLTVPARVAYRLPDNLSFPEAAMLEAVSVALHGVAVSELMGEEVVLVIGAGMIGLL
LLQAARAAGASCVYISDVDETRLKLAEELGADETLLATGAALVEEILRRTDGRGVDMVLE
AVGRDETIAAGIDCVRKGGTVTLVGNISPQITLPLQKVVSRQIRLQGSCASAGEYPQAME
LIARNEIKVASLITAVAPLSDGASWFERLHAREPNLMKIVLDPRSDSSTERAR

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory