Alignments for a candidate for SOT in Granulicella mallensis MP5ACTX8

GapMind for catabolism of small carbon sources

Alignments for a candidate for SOT in Granulicella mallensis MP5ACTX8

Align Sorbitol (D-Glucitol):H+ co-transporter, SOT1 (Km for sorbitol of 0.64 mM) of 509 aas and 12 TMSs (Gao et al. 2003). SOT1 of P. cerasus is expressed throughout fruit development, but especially when growth and sorbitol accumulation rates are highest. In leaves, PcSOT1 expression is highest in young, expanding tissues, but substantially less in mature leaves (characterized)
to candidate WP_150110769.1 ACIX8_RS04220 sugar porter family MFS transporter

Query= TCDB::AIU41385.1
         (509 letters)



>NCBI__GCF_000178955.2:WP_150110769.1
          Length = 472

 Score =  223 bits (569), Expect = 9e-63
 Identities = 153/494 (30%), Positives = 247/494 (50%), Gaps = 48/494 (9%)

Query: 11  VSGQPLLKKKPKRNLYAIGCAILASMTSILLGYDIGVMSGASIYIQEDLKISDVEVEILI 70
           ++G        +R  Y  G AI+A++  +L GYD  V+ GA  + ++   ++      L+
Sbjct: 2   ITGAQSASTPAERTTYVWGIAIVAALGGLLFGYDWVVIGGARQFYEQYFHLTS---PALV 58

Query: 71  GILNLYSL----IGSAAAGRTSDWIGRRYTIVFAGAIFFTGALLMGFATNYAFLMVGRFV 126
           G  N  +L    IGS AAG  +D  GRR  ++ +  +F   + L G+A ++   +V R +
Sbjct: 59  GWANSCALVGCLIGSLAAGFFADRYGRRRVLLVSAVLFAVSSALTGWAYSFNSFIVWRIL 118

Query: 127 AGIGVGYALMIAPVYNAEVSPASSRGALTSFPEVFVNIGILLGYVANYAFSG-LPINL-- 183
            G  +G +  ++P+Y AE+SPA+ RG L S  +  + IGILL  V N+  +  +P NL  
Sbjct: 119 GGTAIGLSSNVSPLYIAEISPAAIRGRLVSLNQFAIVIGILLAQVVNWLIARPVPANLSA 178

Query: 184 -----------GWRLMLGVGVFPSVILAVGVLTMPESPRWLVMQGRLGDAKHVLDKTSDS 232
                      GWR M    V P+++  +  L +PESPRWL+ + R  DA+ VL +    
Sbjct: 179 DVLLHSWNVQYGWRWMFMAVVAPAIVFTIASLFIPESPRWLLTREREADAREVLQRIGGQ 238

Query: 233 LEEAQLRLADIKEAAGIPEHCTEDVVQVPKHSHGEEVWKELLLHPTPPVRHILIAAVGFH 292
           L  A   +  I+ A    E  TE              W+ELL    P VR I++  +G  
Sbjct: 239 LY-ASAEIESIERAIRA-EADTEP-----------SSWRELL---RPSVRRIVLVGIGLA 282

Query: 293 FFQQMSGIDALVLYSPRIFRASGITDSSTLLLATVAVGFSKTIFTLIAIGFLDRVGRRPL 352
             QQ +GI+ L  Y+  ++R++G   ++ +LL  V  G    +FT++A+  +DR+GRR +
Sbjct: 283 VLQQWTGINTLFNYAAEVYRSAGY-GANDILLNIVITGAINLVFTVLAMLLVDRLGRRWM 341

Query: 353 LLTSVAGM-IASLLCLGTSLTIVDHEKEKMMWASVVCLTMVLAYVGFFSIGMGPIAWVYS 411
           +L    G+ ++ LLC             +  W     L + L+ +  +++ + P+ WV  
Sbjct: 342 MLFGCVGIGVSHLLCAFAY---------RAGWRGSAVLVLTLSAIACYALTLAPVTWVLI 392

Query: 412 SEIFPLKLRAQGCSMGTAVNRIMSGVLTMTFITLYKAITMGGTFFLYGAIATVGWVFFYT 471
           SEIFP ++R+ G S   +     S  LT TF  L +++   G FF YG I  +G      
Sbjct: 393 SEIFPNRVRSHGVSAAVSALWAASFALTYTFPILNRSLGTSGIFFCYGLICLLGCGLVAM 452

Query: 472 MLPETQGRTLEDME 485
            +PET+GRTLE +E
Sbjct: 453 FVPETKGRTLEQIE 466


Lambda     K      H
   0.326    0.140    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 712
Number of extensions: 43
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 509
Length of database: 472
Length adjustment: 34
Effective length of query: 475
Effective length of database: 438
Effective search space:   208050
Effective search space used:   208050
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources