GapMind for catabolism of small carbon sources

 

Protein WP_008508827.1 in Brucella inopinata BO1

Annotation: NCBI__GCF_000182725.1:WP_008508827.1

Length: 258 amino acids

Source: GCF_000182725.1 in NCBI

Candidate for 26 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-glucosamine (chitosamine) catabolism AO353_21725 hi ABC transporter for D-Glucosamine, putative ATPase component (characterized) 55% 94% 270 glutamine ABC transporter, ATP-binding protein GlnQ 55% 259.6
L-arginine catabolism artP med histidine transport ATP-binding protein hisP (characterized) 52% 98% 251.1 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-histidine catabolism hisP med histidine transport ATP-binding protein hisP (characterized) 52% 98% 251.1 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-lysine catabolism hisP med histidine transport ATP-binding protein hisP (characterized) 52% 98% 251.1 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-histidine catabolism aapP med ABC transporter for L-Glutamine, L-Histidine, and other L-amino acids, ATPase component (characterized) 50% 91% 245 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-asparagine catabolism aapP med AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 50% 92% 243.4 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-aspartate catabolism aapP med AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 50% 92% 243.4 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-glutamate catabolism aapP med AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 50% 92% 243.4 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-leucine catabolism aapP med AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 50% 92% 243.4 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-proline catabolism aapP med AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 50% 92% 243.4 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-histidine catabolism BPHYT_RS24015 med ABC transporter related (characterized, see rationale) 47% 98% 243 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-citrulline catabolism AO353_03040 med ABC transporter for L-Arginine and L-Citrulline, ATPase component (characterized) 50% 97% 241.9 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-glutamate catabolism gltL med GluA aka CGL1950, component of Glutamate porter (characterized) 52% 97% 241.5 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-asparagine catabolism bztD med BztD, component of Glutamate/glutamine/aspartate/asparagine porter (characterized) 49% 90% 240 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-aspartate catabolism bztD med BztD, component of Glutamate/glutamine/aspartate/asparagine porter (characterized) 49% 90% 240 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
D-alanine catabolism Pf6N2E2_5405 med ABC transporter for D-Alanine, ATPase component (characterized) 50% 93% 238.8 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-citrulline catabolism PS417_17605 med ATP-binding cassette domain-containing protein; SubName: Full=Amino acid transporter; SubName: Full=Histidine ABC transporter ATP-binding protein; SubName: Full=Histidine transport system ATP-binding protein (characterized, see rationale) 51% 91% 234.6 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-asparagine catabolism bgtA med ATPase (characterized, see rationale) 50% 91% 232.3 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-aspartate catabolism bgtA med ATPase (characterized, see rationale) 50% 91% 232.3 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-asparagine catabolism glnQ med Glutamine ABC transporter ATP-binding protein, component of Glutamine transporter, GlnQP. Takes up glutamine, asparagine and glutamate which compete for each other for binding both substrate and the transmembrane protein constituent of the system (Fulyani et al. 2015). Tandem substrate binding domains (SBDs) differ in substrate specificity and affinity, allowing cells to efficiently accumulate different amino acids via a single ABC transporter. Analysis revealed the roles of individual residues in determining the substrate affinity (characterized) 47% 96% 228 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-histidine catabolism bgtA med BgtA aka SLR1735, component of Arginine/lysine/histidine/glutamine porter (characterized) 49% 96% 228 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-asparagine catabolism peb1C med PEB1C, component of Uptake system for glutamate and aspartate (characterized) 49% 97% 227.3 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-aspartate catabolism peb1C med PEB1C, component of Uptake system for glutamate and aspartate (characterized) 49% 97% 227.3 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-asparagine catabolism aatP med ABC transporter for L-asparagine and L-glutamate, ATPase component (characterized) 50% 88% 226.1 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-aspartate catabolism aatP med ABC transporter for L-asparagine and L-glutamate, ATPase component (characterized) 50% 88% 226.1 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0
L-histidine catabolism PA5503 med Methionine import ATP-binding protein MetN 2, component of L-Histidine uptake porter, MetIQN (characterized) 40% 72% 174.1 ABC transporter for D-Glucosamine, putative ATPase component 55% 270.0

Sequence Analysis Tools

View WP_008508827.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MLAASSVEIRKVNKYYGPFHALRNVDLSIIPGKVTCLIGPSGSGKSTLLRCINFLEEYDS
GEIVIDGQLIGYVSPGGRKMPGRKLREMRRSIGMVFQQFNLWPHMTALQNVAEGLIRVRG
LPKSEAEARAAEALKKVGLADKMANHPSRLSGGQQQRVAIARAIAMEPRLMLFDEPTSAL
DPELVGEVLSVMKTLASEGMTMVVVTHEMGFAAHVADQVAFMEKGELVGVGAPSQILHHP
EDPRIESFLRTYHERNSF

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory