L-histidine catabolism in Brucella inopinata BO1

Best path

braC, braD, braE, braF, braG, hutH, hutU, hutI, hutF, hutG'

Rules

Overview: Histidine utilization in GapMind is based on MetaCyc pathways L-histidine degradation I (link) or II (link). These pathways are very similar. Other pathways in MetaCyc (III-VI) are not complete or are not reported in prokaryotes, so they are not included.

• all:
  • histidine-transport, hutH, hutU, hutI and hutG
  • or histidine-transport, hutH, hutU, hutI, hutH, hutF and hutG'
  • Comment: In pathway I, hutH deaminates histidine to urocanate, hutU hydrates it to 4-imidazolone-5-propanoate, hutI hydrolyzes it to N-formiminoglutamate, and hutG hydrolyzes it to formamide and glutamate. Formamide can be cleaved to formate and ammonia, but it could also be secreted, so this is not described. Similarly, formate may be oxidized to CO2 or secreted. Pathway II also involves conversion to N-formiminoglutamate, but then it is deaminated to N-formylglutamate by hutF, and hydrolyzed to formate and glutamate by hutG'.
• histidine-transport:
  • hisP, hisM, hisQ and hisJ
  • or PA5503, PA5504 and PA5505
  • or Ac3H11_2562, Ac3H11_2561, Ac3H11_2560, Ac3H11_2555 and Ac3H11_2554
  • or natA, natB, natC, natD and natE
  • or aapJ, aapQ, aapM and aapP
  • or braC, braD, braE, braF and braG
  • or hutV, hutW and hutX
  • or bgtA and bgtB
  • or BPHYT_RS24000, BPHYT_RS24005, BPHYT_RS24010 and BPHYT_RS24015
  • or permease
  • or Ga0059261_1577
  • or S15A3
  • or LAT2
  • or LHT
  • or SLC38A3
  • or PTR2
  • Comment: Transporters were identified using query: transporter:histidine:L-histidine:his

48 steps (37 with candidates)

Or see definitions of steps

Step	Description	Best candidate	2nd candidate
braC	ABC transporter for glutamate, histidine, arginine, and other amino acids, substrate-binding component BraC	BIBO1_RS12225	BIBO1_RS12220
braD	ABC transporter for glutamate, histidine, arginine, and other amino acids, permease component 1 (BraD)	BIBO1_RS12250	BIBO1_RS14975
braE	ABC transporter for glutamate, histidine, arginine, and other amino acids, permease component 2 (BraE)	BIBO1_RS12245
braF	ABC transporter for glutamate, histidine, arginine, and other amino acids, ATPase component 1 (BraF)	BIBO1_RS12240	BIBO1_RS19765
braG	ABC transporter for glutamate, histidine, arginine, and other amino acids, ATPase component 2 (BraG)	BIBO1_RS12235	BIBO1_RS14990
hutH	histidine ammonia-lyase	BIBO1_RS18875
hutU	urocanase	BIBO1_RS18885
hutI	imidazole-5-propionate hydrolase	BIBO1_RS18870	BIBO1_RS15490
hutF	N-formiminoglutamate deiminase	BIBO1_RS18865
hutG'	N-formylglutamate amidohydrolase	BIBO1_RS18880	BIBO1_RS15185
Alternative steps:
aapJ	L-histidine ABC transporter, substrate-binding component AapJ	BIBO1_RS07165	BIBO1_RS18960
aapM	L-histidine ABC transporter, permease component 2 (AapM)	BIBO1_RS07175	BIBO1_RS19985
aapP	L-histidine ABC transporter, ATPase component AapP	BIBO1_RS07180	BIBO1_RS20000
aapQ	L-histidine ABC transporter, permease component 1 (AapQ)	BIBO1_RS07170	BIBO1_RS19985
Ac3H11_2554	ABC transporter for L-Histidine, permease component 2	BIBO1_RS17105	BIBO1_RS14880
Ac3H11_2555	L-histidine ABC transporter, substrate-binding component 2	BIBO1_RS17110	BIBO1_RS16850
Ac3H11_2560	L-histidine ABC transporter, ATPase component	BIBO1_RS14945	BIBO1_RS17285
Ac3H11_2561	L-histidine ABC transporter, permease component 1	BIBO1_RS14950
Ac3H11_2562	L-histidine ABC transporter, substrate-binding component 1
bgtA	L-histidine ABC transporter, ATPase component BgtA	BIBO1_RS07180	BIBO1_RS20000
bgtB	L-histidine ABC transporter, fused substrate-binding and permease components (BgtB/BgtAB)
BPHYT_RS24000	L-histidine ABC transporter, substrate-binding component	BIBO1_RS07950	BIBO1_RS16850
BPHYT_RS24005	L-histidine ABC transporter, permease component 1	BIBO1_RS07945	BIBO1_RS14880
BPHYT_RS24010	L-histidine ABC transporter, permease component 2	BIBO1_RS07940	BIBO1_RS17105
BPHYT_RS24015	L-histidine ABC transporter, ATPase component	BIBO1_RS14870	BIBO1_RS20000
Ga0059261_1577	L-histidine transporter
hisJ	L-histidine ABC transporter, substrate-binding component HisJ	BIBO1_RS16850	BIBO1_RS16855
hisM	L-histidine ABC transporter, permease component 1 (HisM)	BIBO1_RS07940	BIBO1_RS17105
hisP	L-histidine ABC transporter, ATPase component HisP	BIBO1_RS14870	BIBO1_RS20000
hisQ	L-histidine ABC transporter, permease component 2 (HisQ)	BIBO1_RS07945	BIBO1_RS19985
hutG	N-formiminoglutamate formiminohydrolase	BIBO1_RS07375
hutV	L-histidine ABC transporter, ATPase component HutV	BIBO1_RS18340	BIBO1_RS17920
hutW	L-histidine ABC transporter, permease component HutW	BIBO1_RS18345	BIBO1_RS17915
hutX	L-histidine ABC transporter, substrate-binding component HutX
LAT2	L-histidine transporter
LHT	L-histidine transporter
natA	L-histidine ABC transporter, ATPase component 1 (NatA)	BIBO1_RS19765	BIBO1_RS12240
natB	L-histidine ABC transporter, substrate-binding component NatB
natC	L-histidine ABC transporter, permease component 1 (NatC)
natD	L-histidine ABC transporter, permease component 2 (NatD)	BIBO1_RS19000
natE	L-histidine ABC transporter, ATPase component 2 (NatE)	BIBO1_RS12235	BIBO1_RS14990
PA5503	L-histidine ABC transporter, ATPase component	BIBO1_RS19020	BIBO1_RS19230
PA5504	L-histidine ABC transporter, permease component	BIBO1_RS19025
PA5505	L-histidine ABC transporter, substrate-binding component	BIBO1_RS06710	BIBO1_RS19015
permease	L-histidine permease	BIBO1_RS14845
PTR2	L-histidine:H+ symporter
S15A3	L-histidine transporter
SLC38A3	L-histidine:Na+ symporter

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.