Alignments for a candidate for glcF in Mesorhizobium ciceri WSM1271

GapMind for catabolism of small carbon sources

Alignments for a candidate for glcF in Mesorhizobium ciceri WSM1271

Align D-lactate oxidase, iron-sulfur subunit (EC 1.1.3.15) (characterized)
to candidate YP_004141719.1 Mesci_2526 hypothetical protein

Query= reanno::Smeli:SMc00926
         (443 letters)



>NCBI__GCF_000185905.1:YP_004141719.1
          Length = 441

 Score =  648 bits (1671), Expect = 0.0
 Identities = 314/432 (72%), Positives = 360/432 (83%), Gaps = 2/432 (0%)

Query: 14  LQTNFSPEQLADPHVAESETILRKCVHCGFCTATCPTYVVLGDELDSPRGRIYLIKDMLE 73
           +QTNFS  QLADPHVAESE ILRKCVHCGFCTATCPTYV LG+ELDSPRGRIYLIKDMLE
Sbjct: 1   MQTNFSLAQLADPHVAESEKILRKCVHCGFCTATCPTYVTLGNELDSPRGRIYLIKDMLE 60

Query: 74  NGRAADSETVTHIDRCLSCLSCLTTCPSGVDYMHLVDHARAHIEKTYKRPFKDRLARSVI 133
           NGR AD + VTHIDRCLSCL+C+TTCPSGV+YMHLVDHARAHI++TYKRP  DRL R+++
Sbjct: 61  NGRPADKQIVTHIDRCLSCLACMTTCPSGVNYMHLVDHARAHIQETYKRPLLDRLTRAML 120

Query: 134 AATLPYPSRFRLALGAAGLARPLAGLLKRVPFLRTLGVMLDLAPSALPAARG-AKPAVYA 192
           A  LPYP RFR AL  A L +P  GL  +VP L+ L  ML LAP+++PAA   A P ++A
Sbjct: 121 AFVLPYPQRFRAALKLAKLGKPFIGLFDKVPALKPLSAMLKLAPASIPAASPMASPGIHA 180

Query: 193 AKGTPRARVALLTGCAQPVLRPEINDATIRLLTGQGVEVVVSAGEGCCGALVHHMGRDEQ 252
            +GT R RVA+LTGCAQ VL P IN+ATI LLT  GVEV+V  GEGCCGALVHHMGR++ 
Sbjct: 181 GQGTKRGRVAILTGCAQSVLDPAINEATISLLTRLGVEVLVPPGEGCCGALVHHMGREQA 240

Query: 253 ALQAGRHNIDVWLKAAEEDGLDAIIITASGCGTTIKDYGHMLRLDPAYAEKAARVSALAK 312
           AL + R N+D W +A ++ GLDAI+ITASGCGTTIKDYG MLRLDPAYA+KAARVSALAK
Sbjct: 241 ALASARQNVDAWTRALDQGGLDAIVITASGCGTTIKDYGFMLRLDPAYADKAARVSALAK 300

Query: 313 DVTEYLATLDLPEQGAR-NLTVAYHSACSMQHGQKITSAPKQLLKRAGFSVREPAEGHLC 371
           DVTEYL+TLDLPE   +    VAYHSACSMQHGQKIT  PK+LL +AGF VREP EGHLC
Sbjct: 301 DVTEYLSTLDLPEPVRKPGTVVAYHSACSMQHGQKITRQPKELLAKAGFIVREPREGHLC 360

Query: 372 CGSAGTYNILQPEISAKLKARKVRNIEATKPEVIATGNIGCITQIASGTEIPILHTVELL 431
           CGSAGTYNILQPEISAKL+ RKV+NIEAT   ++ATGNIGCITQIAS   +P++HT++LL
Sbjct: 361 CGSAGTYNILQPEISAKLRDRKVKNIEATGASIVATGNIGCITQIASAAMMPVVHTIKLL 420

Query: 432 DWAYGGPKPAGL 443
           DWAYGGPKP G+
Sbjct: 421 DWAYGGPKPEGV 432


Lambda     K      H
   0.320    0.135    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 645
Number of extensions: 18
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 443
Length of database: 441
Length adjustment: 32
Effective length of query: 411
Effective length of database: 409
Effective search space:   168099
Effective search space used:   168099
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources