Alignments for a candidate for amaB in Ruegeria conchae TW15

GapMind for catabolism of small carbon sources

Alignments for a candidate for amaB in Ruegeria conchae TW15

Align L-piperidine-6-carboxylate dehydrogenase; EC 1.2.1.21 (characterized, see rationale)
to candidate WP_050806438.1 G7G_RS0104210 aldehyde dehydrogenase family protein

Query= uniprot:Q88CC3
         (496 letters)



>NCBI__GCF_000192475.1:WP_050806438.1
          Length = 498

 Score =  672 bits (1733), Expect = 0.0
 Identities = 340/492 (69%), Positives = 394/492 (80%), Gaps = 2/492 (0%)

Query: 6   LERLGVAAEAYTQGDYPVHTPIDGSQIASVKLLGKAETIARIDQAQSAFEAWRSVPAPRR 65
           L +L + A   T G   V +PIDGS +A V     +E  +   +++ AF+AWR VPAPRR
Sbjct: 7   LAKLDLTAAELTGGSLRVTSPIDGSVLAEVHETPLSEMKSIFARSKEAFQAWRVVPAPRR 66

Query: 66  GELVRLFGEVLREHKADLGELVSIEAGKITQEGLGEVQEMIDICDFAVGLSRQLYGLTIA 125
           GEL+RL GE LR  K DLG LVS EAGKIT EGLGEVQEMIDICDFAVGLSRQLYGLTIA
Sbjct: 67  GELIRLLGEELRAAKDDLGALVSWEAGKITSEGLGEVQEMIDICDFAVGLSRQLYGLTIA 126

Query: 126 SERPGHHMRETWHPLGVVGVISAFNFPVAVWAWNTALALVAGNSVVWKPSEKTPLTALAC 185
           SERPGH M ETWHP G VGVISAFNFPVAVW+WN ALALV G+ V+WKPSEKTPLTA+AC
Sbjct: 127 SERPGHRMMETWHPAGPVGVISAFNFPVAVWSWNAALALVCGDPVIWKPSEKTPLTAMAC 186

Query: 186 QALFEKALKAFGDAPAGLAQLVIGGREAGEAMVDDPRVPLVSATGSTRMGREVGPRVAAR 245
           Q +FE+AL  FGDAP GL Q +IGG   GEA+V    VP++SATGSTRMGR V P VA R
Sbjct: 187 QKIFERALARFGDAPVGLLQTLIGGAALGEALVASEDVPVISATGSTRMGRAVAPIVAQR 246

Query: 246 FGRSILELGGNNAMILAPSADLDLAVRGILFSAVGTAGQRCTTLRRLIVHRSIKDEVVAR 305
           FG+ ILELGGNNAMI+ PSADL++AVR I+FSAVGTAGQRCTTLRRLIVH SI++E+VAR
Sbjct: 247 FGKCILELGGNNAMIVGPSADLEMAVRAIVFSAVGTAGQRCTTLRRLIVHNSIREELVAR 306

Query: 306 VKAAYGKVRIGDP-RKDNLVGPLIDKQSFDAMQGALAKARDEGGQVFGGERQLADQYPNA 364
           +K AY  + IG+P  +  L+GPL+D+ S DAM GAL +A  EGG VFGG+R + +  P  
Sbjct: 307 LKKAYSGLPIGNPLTEGTLIGPLVDEASGDAMTGALKRAEAEGGTVFGGKR-VTEGMPGG 365

Query: 365 YYVSPAIAEMPAQSDVVRHETFAPILYVLAYDDFEEALRLNNEVPQGLSSCIFTTDIREA 424
            Y+ PAI EMP+Q+  V+ ETFAPILYV+ YDDF EA+ L N+VPQGLSSC+FT ++REA
Sbjct: 366 VYMEPAIVEMPSQTATVKTETFAPILYVMGYDDFAEAIELQNDVPQGLSSCVFTLNMREA 425

Query: 425 ERFQSASGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKGYMRRQTNTVNYS 484
           E+F +A+GSDCGIANVNIG SGAEIGGAFGGEKETGGGRESGSDAWK YMRRQTNTVNYS
Sbjct: 426 EQFLTAAGSDCGIANVNIGPSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQTNTVNYS 485

Query: 485 RELPLAQGIVFD 496
            ELPLAQG+ FD
Sbjct: 486 AELPLAQGVKFD 497


Lambda     K      H
   0.318    0.135    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 820
Number of extensions: 33
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 496
Length of database: 498
Length adjustment: 34
Effective length of query: 462
Effective length of database: 464
Effective search space:   214368
Effective search space used:   214368
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources