Alignments for a candidate for dauA in Bacteroides fluxus YIT 12057

GapMind for catabolism of small carbon sources

Alignments for a candidate for dauA in Bacteroides fluxus YIT 12057

Align C4-dicarboxylic acid transporter DauA; Dicarboxylic acid uptake system A (characterized)
to candidate WP_039969414.1 HMPREF9446_RS12270 sulfate permease

Query= SwissProt::P0AFR2
         (559 letters)



>NCBI__GCF_000195635.1:WP_039969414.1
          Length = 558

 Score =  355 bits (910), Expect = e-102
 Identities = 193/506 (38%), Positives = 305/506 (60%), Gaps = 43/506 (8%)

Query: 22  EKYTAARFTRDLIAGITVGIIAIPLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFS 81
           + Y+   F  DL+AGI VGI+A+PLA+A  I SGV+P+ G+ TA +AG +++L GGS+  
Sbjct: 15  KNYSKETFMADLMAGIIVGIVALPLAIAFGIASGVSPEKGIITAIIAGFIVSLLGGSKVQ 74

Query: 82  VSGPTAAFVVILYPVSQQFGLAGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTS 141
           + GPT AF+VI+Y + QQ+G +GL+VATL++G+ LIL+G+ + G +I++IP  + +GFTS
Sbjct: 75  IGGPTGAFIVIIYGIIQQYGESGLIVATLMAGVILILLGVFKLGAVIKFIPYPIIIGFTS 134

Query: 142 GIGITIGTMQIKDFLGLQMAH--VPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFW 199
           GI +TI T QI D  GL      VP  ++ K    F    T+N  + A+ I ++ I+   
Sbjct: 135 GIAVTIFTTQIADVFGLTFGEDKVPGDFVGKWIVYFRHFDTVNWWNTAVSIFSIIIIAIT 194

Query: 200 PRLGIRLPGHLPALLAGCAVMGIVNLLGG--HVATIGSQFHYVLADGSQGNGIPQLLPQL 257
           PR   ++PG L A++   A + ++ + GG   + TIG +F            I   LP  
Sbjct: 195 PRFSKKIPGSLIAIVVVTAAVYLMKVYGGIDCIDTIGDRF-----------SIRAELPDA 243

Query: 258 VLPWDLPNSEFTLTWDSIRTLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQ 317
           V+P         L W++++ L P A ++A+LGAIESLL A V DG+ G KH +N+EL+ Q
Sbjct: 244 VMP--------ALDWEAVKDLFPVAITIAVLGAIESLLSATVADGVIGDKHDSNTELIAQ 295

Query: 318 GLGNIIAPFFGGITATAAIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLS 377
           G  N++ P FGGI AT AIAR+ AN+  G  +P++ ++H+++++L LL+L PL  ++P+ 
Sbjct: 296 GAANLVTPLFGGIPATGAIARTMANINNGGKTPVAGIVHAVVLLLILLLLMPLAQYIPMG 355

Query: 378 AMAALLLMVAWNMSEAHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLL 437
            +A +L++V++NMS       LLR+ PK D+ V+L+   LTV+FD+ IAI VG+V+A +L
Sbjct: 356 CLAGVLVIVSYNMSGWRTFKGLLRN-PKSDVTVLLITFFLTVIFDLTIAIEVGLVIACVL 414

Query: 438 FMRRIARMTRLAPVVVD------------------VPDDVLVLRVIGPLFFAAAEGLFTD 479
           FMRR+   T ++ V+ D                  +P+ V V  + GP FF  A      
Sbjct: 415 FMRRVMETTEIS-VIKDEIDPNAELDIATNEEHLVIPEGVEVYEINGPYFFGIATKFEET 473

Query: 480 LESRLEGKRIVILKWDAVPVLDAGGL 505
           +    +  ++ I++   VP +D+ G+
Sbjct: 474 MAQLGDRPKVRIIRMRKVPFIDSTGI 499


Lambda     K      H
   0.328    0.142    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 657
Number of extensions: 27
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 559
Length of database: 558
Length adjustment: 36
Effective length of query: 523
Effective length of database: 522
Effective search space:   273006
Effective search space used:   273006
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources