GapMind for catabolism of small carbon sources

 

Protein WP_010529728.1 in Lentibacillus jeotgali Grbi

Annotation: NCBI__GCF_000224785.1:WP_010529728.1

Length: 489 amino acids

Source: GCF_000224785.1 in NCBI

Candidate for 37 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-arginine catabolism patD med aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized) 43% 99% 424.5 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-citrulline catabolism patD med aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized) 43% 99% 424.5 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
putrescine catabolism patD med aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized) 43% 99% 424.5 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
4-hydroxybenzoate catabolism adh med aldehyde dehydrogenase ALDH; EC 1.2.1.3 (characterized) 44% 95% 422.2 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
2'-deoxyinosine catabolism adh med aldehyde dehydrogenase ALDH; EC 1.2.1.3 (characterized) 44% 95% 422.2 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
2-deoxy-D-ribose catabolism adh med aldehyde dehydrogenase ALDH; EC 1.2.1.3 (characterized) 44% 95% 422.2 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
ethanol catabolism adh med aldehyde dehydrogenase ALDH; EC 1.2.1.3 (characterized) 44% 95% 422.2 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-threonine catabolism adh med aldehyde dehydrogenase ALDH; EC 1.2.1.3 (characterized) 44% 95% 422.2 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
thymidine catabolism adh med aldehyde dehydrogenase ALDH; EC 1.2.1.3 (characterized) 44% 95% 422.2 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-tryptophan catabolism adh med aldehyde dehydrogenase ALDH; EC 1.2.1.3 (characterized) 44% 95% 422.2 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-phenylalanine catabolism pad-dh med aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized) 45% 91% 399.8 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-fucose catabolism aldA med NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 42% 94% 380.2 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-rhamnose catabolism aldA med NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 42% 94% 380.2 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-threonine catabolism aldA med NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 42% 94% 380.2 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
4-hydroxybenzoate catabolism praB med 2-hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85) (characterized) 43% 97% 375.6 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-tryptophan catabolism praB med 2-hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85) (characterized) 43% 97% 375.6 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-arginine catabolism kauB med 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized) 42% 95% 370.5 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-arginine catabolism puuC med 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized) 42% 95% 370.5 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-citrulline catabolism puuC med 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized) 42% 95% 370.5 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
putrescine catabolism puuC med 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized) 42% 95% 370.5 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-tryptophan catabolism nbaE med 2-aminomuconate semialdehyde dehydrogenase (EC 1.2.1.32) (characterized) 42% 99% 355.5 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-arabinose catabolism xacF lo Alpha-ketoglutarate semialdehyde dehydrogenase; KGSADH; EC 1.2.1.26 (characterized) 39% 99% 353.2 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
D-galacturonate catabolism dopDH lo Alpha-ketoglutarate semialdehyde dehydrogenase; KGSADH; EC 1.2.1.26 (characterized) 39% 99% 353.2 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
D-glucuronate catabolism dopDH lo Alpha-ketoglutarate semialdehyde dehydrogenase; KGSADH; EC 1.2.1.26 (characterized) 39% 99% 353.2 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
D-xylose catabolism dopDH lo Alpha-ketoglutarate semialdehyde dehydrogenase; KGSADH; EC 1.2.1.26 (characterized) 39% 99% 353.2 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-lysine catabolism patD lo aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized) 40% 99% 340.9 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-arabinose catabolism aldA lo lactaldehyde dehydrogenase (EC 1.2.1.22); D-glyceraldehyde dehydrogenase (NADP+) (EC 1.2.1.89) (characterized) 36% 97% 309.7 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
D-xylose catabolism aldA lo lactaldehyde dehydrogenase (EC 1.2.1.22); D-glyceraldehyde dehydrogenase (NADP+) (EC 1.2.1.89) (characterized) 36% 97% 309.7 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-valine catabolism mmsA lo Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (characterized) 32% 93% 252.3 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-isoleucine catabolism iolA lo malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) 31% 90% 243.4 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
myo-inositol catabolism mmsA lo malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) 31% 90% 243.4 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
propionate catabolism iolA lo malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) 31% 90% 243.4 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-threonine catabolism iolA lo malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) 31% 90% 243.4 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-valine catabolism iolA lo malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) 31% 90% 243.4 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-lysine catabolism amaB lo Alpha-aminoadipic semialdehyde dehydrogenase; Alpha-AASA dehydrogenase; Aldehyde dehydrogenase family 7 member A1; Antiquitin-1; Betaine aldehyde dehydrogenase; Delta1-piperideine-6-carboxylate dehydrogenase; P6c dehydrogenase; EC 1.2.1.31; EC 1.2.1.3; EC 1.2.1.8 (characterized) 33% 86% 235.7 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-arginine catabolism astD lo N-succinylglutamate 5-semialdehyde dehydrogenase; Succinylglutamic semialdehyde dehydrogenase; SGSD; EC 1.2.1.71 (characterized) 34% 91% 211.5 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9
L-citrulline catabolism astD lo N-succinylglutamate 5-semialdehyde dehydrogenase; Succinylglutamic semialdehyde dehydrogenase; SGSD; EC 1.2.1.71 (characterized) 34% 91% 211.5 Betaine aldehyde dehydrogenase; BADH; Glycine betaine aldehyde dehydrogenase; EC 1.2.1.8 69% 694.9

Sequence Analysis Tools

View WP_010529728.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

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Sequence

MDIKKQLIDGSWVTSNSDAVRDIINPFNQKIVATVTESDESDTKAAITAARKAFDQGDWA
TTPAAERGKIVHKIADLIERDREELAELESLDTGKTVEESLGDMDDIAGVFRYFAEMADK
DGGEIIESPIPNSTSKVVHEPVGVCGQITPWNYPLLQASWKLAPALAAGNTLVMKPSEIT
PLTSIKVFELMAEAGVPAGVVNLVLGPGDSVGAELSANDNVDLISFTGGIHTGKKIMQAA
SSNVKNLALELGGKNPNIVFADADFETAVDQAMNAVFFHAGQICSAGTRLIIEESIHDAF
VSAIVDRVKNIKLGSGFDESTQMGPLISEDHLNKVVNYVENGKQEGATVAVGGSRPDDPE
LQNGLFYLPTILTGCTSDMRVVQDEGFGPVITVEKFTTEEEAVRLANDSIYGLSGGVFTN
DIAKAERCVAKLRMGTVWINDVNLYFPHAPWGGYKQSGIGRELGKTGLEEYQETKHIFQN
LKPEPVNWF

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory