Protein WP_010529795.1 in Lentibacillus jeotgali Grbi
Annotation: NCBI__GCF_000224785.1:WP_010529795.1
Length: 492 amino acids
Source: GCF_000224785.1 in NCBI
Candidate for 29 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| N-acetyl-D-glucosamine catabolism | nagPcb | hi | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- (characterized) | 43% | 100% | 389 | protein-Npi-phosphohistidine-N-acetyl-D-glucosamine phosphotransferase (EC 2.7.1.193) | 40% | 365.9 |
| D-glucosamine (chitosamine) catabolism | nagPcb | hi | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- (characterized) | 43% | 100% | 389 | protein-Npi-phosphohistidine-N-acetyl-D-glucosamine phosphotransferase (EC 2.7.1.193) | 40% | 365.9 |
| N-acetyl-D-glucosamine catabolism | nagEcba | med | protein-Npi-phosphohistidine-N-acetyl-D-glucosamine phosphotransferase (EC 2.7.1.193) (characterized) | 40% | 72% | 365.9 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| D-glucosamine (chitosamine) catabolism | gamP | med | protein-Npi-phosphohistidine-N-acetyl-D-glucosamine phosphotransferase (EC 2.7.1.193) (characterized) | 40% | 72% | 365.9 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| D-glucosamine (chitosamine) catabolism | nagEcba | med | protein-Npi-phosphohistidine-N-acetyl-D-glucosamine phosphotransferase (EC 2.7.1.193) (characterized) | 40% | 72% | 365.9 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| N-acetyl-D-glucosamine catabolism | nagEcb | med | N-acetylglucosamine-specific PTS system, IIBC components (nagE) (characterized) | 42% | 78% | 360.9 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| D-glucosamine (chitosamine) catabolism | nagEcb | med | N-acetylglucosamine-specific PTS system, IIBC components (nagE) (characterized) | 42% | 78% | 360.9 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| N-acetyl-D-glucosamine catabolism | ptsC | med | IIC' aka PtsC2, component of N-Acetylglucosamine (NAG) porter (PtsBC1C2)(also may facilitate xylose transport) (characterized) | 47% | 97% | 335.1 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| D-glucosamine (chitosamine) catabolism | ptsC | med | IIC' aka PtsC2, component of N-Acetylglucosamine (NAG) porter (PtsBC1C2)(also may facilitate xylose transport) (characterized) | 47% | 97% | 335.1 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| N-acetyl-D-glucosamine catabolism | ptsB | med | IIB aka PtsB, component of N-Acetylglucosamine (NAG) porter (PtsBC1C2)(also may facilitate xylose transport) (characterized) | 43% | 87% | 65.5 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| D-glucosamine (chitosamine) catabolism | ptsB | med | IIB aka PtsB, component of N-Acetylglucosamine (NAG) porter (PtsBC1C2)(also may facilitate xylose transport) (characterized) | 43% | 87% | 65.5 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| N-acetyl-D-glucosamine catabolism | nagEIIA | lo | PTS system glucose-specific EIICBA component; EC 2.7.1.-; EC 2.7.1.69 (characterized) | 37% | 72% | 337.4 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| D-cellobiose catabolism | ptsG-crr | lo | PTS system glucose-specific EIICBA component; EC 2.7.1.-; EC 2.7.1.69 (characterized) | 37% | 72% | 337.4 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| D-glucosamine (chitosamine) catabolism | nagEIIA | lo | PTS system glucose-specific EIICBA component; EC 2.7.1.-; EC 2.7.1.69 (characterized) | 37% | 72% | 337.4 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| D-glucose catabolism | ptsG-crr | lo | PTS system glucose-specific EIICBA component; EC 2.7.1.-; EC 2.7.1.69 (characterized) | 37% | 72% | 337.4 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| lactose catabolism | ptsG-crr | lo | PTS system glucose-specific EIICBA component; EC 2.7.1.-; EC 2.7.1.69 (characterized) | 37% | 72% | 337.4 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| D-maltose catabolism | malEIIA | lo | PTS system glucose-specific EIICBA component; EC 2.7.1.-; EC 2.7.1.69 (characterized) | 37% | 72% | 337.4 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| D-maltose catabolism | ptsG-crr | lo | PTS system glucose-specific EIICBA component; EC 2.7.1.-; EC 2.7.1.69 (characterized) | 37% | 72% | 337.4 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| sucrose catabolism | ptsG-crr | lo | PTS system glucose-specific EIICBA component; EC 2.7.1.-; EC 2.7.1.69 (characterized) | 37% | 72% | 337.4 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| trehalose catabolism | ptsG-crr | lo | PTS system glucose-specific EIICBA component; EC 2.7.1.-; EC 2.7.1.69 (characterized) | 37% | 72% | 337.4 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| trehalose catabolism | treEIIA | lo | PTS system glucose-specific EIICBA component; EC 2.7.1.-; EC 2.7.1.69 (characterized) | 37% | 72% | 337.4 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| D-cellobiose catabolism | ptsG | lo | protein-Npi-phosphohistidine-D-glucose phosphotransferase (EC 2.7.1.199) (characterized) | 36% | 97% | 301.2 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| D-glucose catabolism | ptsG | lo | protein-Npi-phosphohistidine-D-glucose phosphotransferase (EC 2.7.1.199) (characterized) | 36% | 97% | 301.2 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| lactose catabolism | ptsG | lo | protein-Npi-phosphohistidine-D-glucose phosphotransferase (EC 2.7.1.199) (characterized) | 36% | 97% | 301.2 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| D-maltose catabolism | ptsG | lo | protein-Npi-phosphohistidine-D-glucose phosphotransferase (EC 2.7.1.199) (characterized) | 36% | 97% | 301.2 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| sucrose catabolism | ptsG | lo | protein-Npi-phosphohistidine-D-glucose phosphotransferase (EC 2.7.1.199) (characterized) | 36% | 97% | 301.2 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| trehalose catabolism | ptsG | lo | protein-Npi-phosphohistidine-D-glucose phosphotransferase (EC 2.7.1.199) (characterized) | 36% | 97% | 301.2 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| D-maltose catabolism | malEIICBA | lo | PTS system, IIABC components (characterized, see rationale) | 30% | 75% | 207.6 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
| D-maltose catabolism | malEIICB | lo | The Maltose group translocator, MalT of 470 aas and 10 TMSs. Takes up extracellular maltose, releasing maltose-phosphate into the cytoplasm (characterized) | 30% | 61% | 124.8 | PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- | 42% | 373.2 |
Sequence Analysis Tools
View WP_010529795.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MMKYLQNLGRSLMLPVAVLPAAAILVGISKYIEKIDGTTAGTIATFLNSGGLAILDNLGI
LFAVGVAIGMSKEQDGAAGLSGLVAWLVATTVLSVSGVAGLQGIEESAVNPAFENIQNVF
IGILSGIVASIMYNRFSHVRLPDALAFFSGKRLVPIMSAASMMVVSVLLLFVWPVVYNAL
VSFGEAIISLEAVGAGIYGFLNRLLIPTGLHHALNSVFWFDVAGIDDIASFWASEGEKGV
TGRYLAGFFPIMMFGLPAAALAMYHTAKTKRKKQAASLLMAAAFASFFTGVTEALEFSFM
FVAPVLYVVHAALTGLSLAVAAMFEWTAGFSFSAGLVDYTLSLGIPIANQPYMLILQGLV
FAVLYYFLFRFLIVKLDLNTPGRESDEPGEGEVDESYNVEEGTLASEKDETAVMAEEIYQ
GLGGDENVRTVDNCVSRLRLEVDDMDKVDQDKIKATGVPGVNVVGKHNVQVVVGTQVQFV
ADEIRKIRKRDE
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory