Annotation: NCBI__GCF_000224785.1:WP_010531399.1
Length: 512 amino acids
Source: GCF_000224785.1 in NCBI
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|---|---|---|---|---|---|---|---|---|
| phenylacetate catabolism | ppa | med | Phenylacetate permease, Ppa (characterized) | 48% | 99% | 479.9 | Monocarboxylic acid transporter | 48% | 473.0 |
| pyruvate catabolism | actP | med | Acetate permease; SubName: Full=Actetate permease; SubName: Full=Cation acetate symporter; SubName: Full=Cation/acetate symporter (characterized, see rationale) | 48% | 94% | 473.8 | Phenylacetate permease, Ppa | 48% | 479.9 |
| acetate catabolism | actP | med | Monocarboxylic acid transporter (characterized) | 48% | 94% | 473 | Phenylacetate permease, Ppa | 48% | 479.9 |
| propionate catabolism | mctC | med | Monocarboxylic acid transporter (characterized) | 48% | 94% | 473 | Phenylacetate permease, Ppa | 48% | 479.9 |
| pyruvate catabolism | mctC | med | Monocarboxylic acid transporter (characterized) | 48% | 94% | 473 | Phenylacetate permease, Ppa | 48% | 479.9 |
| L-lactate catabolism | Shew_2731 | lo | actP-like component of L-lactate and L-malate uptake system (characterized) | 31% | 51% | 138.3 | Phenylacetate permease, Ppa | 48% | 479.9 |
View WP_010531399.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
MSPTVIVLFLIIVALTLVITFWASRQTQTTGDFYTAGGGLTGFQNGLAISGDYLSAASFL GIAGSIALFGFDGFFYSIGFLIAYLVVLFLVAEPLRNLGKYTLADMINARFDAKKVRGAA ALSTITISIFYMLAQLVGAGALIQLLFDIPYTVAVLIVGVMMTIYVLFGGMIATSWVQIV KAVLLMAGMLILSFLVLMHFNFNIGAMFEEAKTATAHGADYLNPGLKYTAPLDTISLMLA LVLGTAGLPHILMRFFTVKDAKTARKSVVTATWIIGAFYVLTLFLGFGAAIFVGEGTIRE ANPGGNMAAPLLAEAIGGEFLFAFISAVAFATILAVVAGLVLSGASAFAHDLYGQIIKNG KASDQQQMLAARYAALAVSVLSIVLSLFAQYLNVAFLVSLAFCIGASANLPVILYTVYWK RFNTTGAVSALLSGLFSALILVSVSPSVFSPTEGAALFVGNPIFPLSNPALVSVPIGFLG GYLGTIFSSKSDAKRYAEVKVKAHTGYRETGS
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory