GapMind for catabolism of small carbon sources

 

Alignments for a candidate for puuC in Lentibacillus jeotgali Grbi

Align 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized)
to candidate WP_010532097.1 ON01_RS16395 aldehyde dehydrogenase family protein

Query= metacyc::MONOMER-11560
         (497 letters)



>NCBI__GCF_000224785.1:WP_010532097.1
          Length = 506

 Score =  355 bits (910), Expect = e-102
 Identities = 206/479 (43%), Positives = 284/479 (59%), Gaps = 18/479 (3%)

Query: 23  FINGEYTDAVSGETFECLSPVDGRFLAKVASCDLADANRAVENARATFNSGVWSQLAPAK 82
           +I GEY    +G+ FE +SPV G+   ++A     D   AV+   A  ++  W+Q + A+
Sbjct: 22  YIGGEYRPPANGKYFENVSPVTGKVFCELARSTKEDVEAAVDAGYAAKDA--WAQTSVAE 79

Query: 83  RKAKLIRFADLLRKNVEELALLETLDMGKPIGDSSSIDIPGAAQAIHWTAEAIDKVYDEV 142
           R   L + AD + +N+E LA+ ET D GK + +  + DIP A     + A AI      V
Sbjct: 80  RADILNKIADRMEENLESLAVAETWDNGKAVREGLAADIPLAVDHFRYFAGAIRAQEGGV 139

Query: 143 APTPHDQLGLVTREPVGVVGAIVPWNFPLLMACWKLGPALATGNSVVLKPSEKSPLTAIR 202
           +   +D +     EP+GVVG I+PWNFP+LMA WKL PALA GN VVLKP+E++P  +I 
Sbjct: 140 SQIDNDTVAYHFHEPLGVVGQIIPWNFPILMATWKLAPALAAGNCVVLKPAEQTP-ASIH 198

Query: 203 IAQLAIEAGIPAGVLNVLPGYGHTVGKALALHMDVDTLVFTGSTKIAKQLMVYAGESNMK 262
           +    I+  +PAGVLN++ G+G   GK LA +  +  + FTG T   + +M YA E N+ 
Sbjct: 199 VLLDLIKDLLPAGVLNIVNGFGVEAGKPLASNSRISKIAFTGETTTGRLIMQYASE-NII 257

Query: 263 RIWLEAGGKSPNIVFADAPD-----LQAAAEAAASAIAFNQGEVCTAGSRLLVERSIKDK 317
            + LE GGKSPNI F D  D     L  A E      A NQGEVCT  SR LV  SI D+
Sbjct: 258 PVTLELGGKSPNIFFPDVMDKDDGFLDKAIEGLV-MFALNQGEVCTCPSRALVHESIYDE 316

Query: 318 FLPMVVEALKGWKPGNPLDPQTTVGALVDTQQMNTVLSYIEAGHKDGAKLLAGG-----K 372
           F+   ++ +   K G+PLD  T +GA    +QM  + SY++ G ++GA++L GG     K
Sbjct: 317 FMERAIKRVNEIKIGHPLDTDTMMGAQASLEQMEKIKSYLDIGRQEGAEVLVGGGVNQLK 376

Query: 373 RTLEETGGTYVEPTIFDGVTNAMRIAQEEIFGPVLSVIAFDTAEEAVAIANDTPYGLAAG 432
             +EE  G Y+EPTIF G  N MRI QEEIFGPVL+V  F+  +EA+ IANDT YGL AG
Sbjct: 377 GDMEE--GYYIEPTIFKG-DNKMRIFQEEIFGPVLAVTTFNENDEAMKIANDTLYGLGAG 433

Query: 433 IWTSDISKAHKTARAVRAGSVWVNQYDGGDMTAPFGGFKQSGNGRDKSLHALEKYTELK 491
           +WT +I+ A++  R + AG VW+N Y      A FGG+K+SG GR+  L  L+ Y + K
Sbjct: 434 VWTRNINTAYRFGRGIEAGRVWMNCYHQYPAHAAFGGYKKSGIGRENHLMMLDHYQQTK 492


Lambda     K      H
   0.316    0.132    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 644
Number of extensions: 28
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 497
Length of database: 506
Length adjustment: 34
Effective length of query: 463
Effective length of database: 472
Effective search space:   218536
Effective search space used:   218536
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory