| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-glutamate catabolism | gltL | hi | GluA aka CGL1950, component of Glutamate porter (characterized) | 65% | 100% | 319.3 | uncharacterized amino-acid ABC transporter ATP-binding protein yhdZ | 58% | 280.4 |
| L-asparagine catabolism | bztD | med | BztD, component of Glutamate/glutamine/aspartate/asparagine porter (characterized) | 59% | 95% | 295.8 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-aspartate catabolism | bztD | med | BztD, component of Glutamate/glutamine/aspartate/asparagine porter (characterized) | 59% | 95% | 295.8 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-histidine catabolism | aapP | med | ABC transporter for L-Glutamine, L-Histidine, and other L-amino acids, ATPase component (characterized) | 60% | 94% | 286.2 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-asparagine catabolism | aapP | med | AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 59% | 95% | 283.1 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-aspartate catabolism | aapP | med | AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 59% | 95% | 283.1 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-glutamate catabolism | aapP | med | AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 59% | 95% | 283.1 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-leucine catabolism | aapP | med | AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 59% | 95% | 283.1 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-proline catabolism | aapP | med | AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 59% | 95% | 283.1 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| D-alanine catabolism | Pf6N2E2_5405 | med | ABC transporter for D-Alanine, ATPase component (characterized) | 57% | 98% | 275 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-asparagine catabolism | aatP | med | Glutamate/aspartate transport ATP-binding protein GltL aka B0652, component of Glutamate/aspartate porter (characterized) | 59% | 99% | 274.2 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-aspartate catabolism | aatP | med | Glutamate/aspartate transport ATP-binding protein GltL aka B0652, component of Glutamate/aspartate porter (characterized) | 59% | 99% | 274.2 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-lysine catabolism | hisP | med | Amino-acid ABC transporter, ATP-binding protein (characterized, see rationale) | 54% | 96% | 271.6 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-asparagine catabolism | bgtA | med | ATPase (characterized, see rationale) | 57% | 93% | 268.5 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-aspartate catabolism | bgtA | med | ATPase (characterized, see rationale) | 57% | 93% | 268.5 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-arginine catabolism | artP | med | Probable ATP-binding component of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized) | 52% | 97% | 252.7 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-histidine catabolism | hisP | med | Probable ATP-binding component of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized) | 52% | 97% | 252.7 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-asparagine catabolism | glnQ | med | Glutamine ABC transporter ATP-binding protein, component of Glutamine transporter, GlnQP. Takes up glutamine, asparagine and glutamate which compete for each other for binding both substrate and the transmembrane protein constituent of the system (Fulyani et al. 2015). Tandem substrate binding domains (SBDs) differ in substrate specificity and affinity, allowing cells to efficiently accumulate different amino acids via a single ABC transporter. Analysis revealed the roles of individual residues in determining the substrate affinity (characterized) | 52% | 98% | 251.9 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-asparagine catabolism | peb1C | med | PEB1C, component of Uptake system for glutamate and aspartate (characterized) | 55% | 99% | 250.4 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-aspartate catabolism | peb1C | med | PEB1C, component of Uptake system for glutamate and aspartate (characterized) | 55% | 99% | 250.4 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-histidine catabolism | BPHYT_RS24015 | med | ABC transporter related (characterized, see rationale) | 50% | 97% | 250 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| D-glucosamine (chitosamine) catabolism | AO353_21725 | med | ABC transporter for D-Glucosamine, putative ATPase component (characterized) | 48% | 97% | 240.4 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-histidine catabolism | bgtA | med | BgtA aka SLR1735, component of Arginine/lysine/histidine/glutamine porter (characterized) | 48% | 98% | 229.2 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-citrulline catabolism | AO353_03040 | med | ABC transporter for L-Arginine and L-Citrulline, ATPase component (characterized) | 47% | 99% | 223.8 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-histidine catabolism | PA5503 | med | Methionine import ATP-binding protein MetN 2, component of L-Histidine uptake porter, MetIQN (characterized) | 43% | 72% | 186 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| putrescine catabolism | potA | lo | PotG aka B0855, component of Putrescine porter (characterized) | 38% | 63% | 175.6 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-proline catabolism | proV | lo | glycine betaine/l-proline transport atp-binding protein prov (characterized) | 37% | 61% | 166.8 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| D-maltose catabolism | malK | lo | Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 (characterized) | 38% | 64% | 161 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| D-sorbitol (glucitol) catabolism | mtlK | lo | ABC transporter for D-Sorbitol, ATPase component (characterized) | 35% | 67% | 160.6 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| trehalose catabolism | thuK | lo | Trehalose import ATP-binding protein SugC; EC 7.5.2.- (characterized) | 38% | 60% | 157.1 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| sucrose catabolism | thuK | lo | ABC transporter (characterized, see rationale) | 34% | 61% | 151 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-fucose catabolism | SM_b21106 | lo | ABC transporter for L-Fucose, ATPase component (characterized) | 36% | 64% | 150.6 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-histidine catabolism | hutV | lo | ABC transporter for L-Histidine, ATPase component (characterized) | 38% | 81% | 150.2 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| D-mannitol catabolism | mtlK | lo | SmoK aka POLK, component of Hexitol (glucitol; mannitol) porter (characterized) | 35% | 71% | 150.2 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| xylitol catabolism | Dshi_0546 | lo | ABC transporter for Xylitol, ATPase component (characterized) | 36% | 69% | 150.2 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| lactose catabolism | lacK | lo | ABC transporter for Lactose, ATPase component (characterized) | 35% | 66% | 149.1 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-arabinose catabolism | xacK | lo | Xylose/arabinose import ATP-binding protein XacK; EC 7.5.2.13 (characterized, see rationale) | 36% | 60% | 148.7 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-tryptophan catabolism | ecfA2 | lo | Energy-coupling factor transporter ATP-binding protein EcfA2; Short=ECF transporter A component EcfA2; EC 7.-.-.- (characterized, see rationale) | 37% | 87% | 148.7 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| D-cellobiose catabolism | gtsD | lo | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 37% | 61% | 148.3 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| D-glucose catabolism | gtsD | lo | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 37% | 61% | 148.3 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| lactose catabolism | gtsD | lo | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 37% | 61% | 148.3 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| D-maltose catabolism | gtsD | lo | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 37% | 61% | 148.3 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| sucrose catabolism | gtsD | lo | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 37% | 61% | 148.3 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| trehalose catabolism | gtsD | lo | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 37% | 61% | 148.3 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| D-xylose catabolism | gtsD | lo | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 37% | 61% | 148.3 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| L-arabinose catabolism | xacJ | lo | Xylose/arabinose import ATP-binding protein XacJ; EC 7.5.2.13 (characterized, see rationale) | 35% | 61% | 147.1 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| D-maltose catabolism | thuK | lo | Sugar-binding transport ATP-binding protein aka MalK1 aka TT_C0211, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized) | 35% | 62% | 144.4 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| D-mannose catabolism | TT_C0211 | lo | Sugar-binding transport ATP-binding protein aka MalK1 aka TT_C0211, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized) | 35% | 62% | 144.4 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| D-fructose catabolism | frcA | lo | Fructose import ATP-binding protein FrcA; EC 7.5.2.- (characterized) | 33% | 85% | 117.9 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| D-mannose catabolism | frcA | lo | Fructose import ATP-binding protein FrcA; EC 7.5.2.- (characterized) | 33% | 85% | 117.9 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| D-ribose catabolism | frcA | lo | Fructose import ATP-binding protein FrcA; EC 7.5.2.- (characterized) | 33% | 85% | 117.9 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| sucrose catabolism | frcA | lo | Fructose import ATP-binding protein FrcA; EC 7.5.2.- (characterized) | 33% | 85% | 117.9 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
| citrate catabolism | fecE | lo | iron(III) dicitrate transport ATP-binding protein FecE (characterized) | 32% | 84% | 110.5 | GluA aka CGL1950, component of Glutamate porter | 65% | 319.3 |
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know