Alignments for a candidate for PS417_12065 in Collinsella tanakaei YIT 12063

GapMind for catabolism of small carbon sources

Alignments for a candidate for PS417_12065 in Collinsella tanakaei YIT 12063

Align D-ribose transporter ATP-binding protein; SubName: Full=Putative xylitol transport system ATP-binding protein; SubName: Full=Sugar ABC transporter ATP-binding protein (characterized, see rationale)
to candidate WP_009141256.1 HMPREF9452_RS06110 ABC transporter ATP-binding protein

Query= uniprot:A0A1N7TX47
         (495 letters)



>NCBI__GCF_000225705.1:WP_009141256.1
          Length = 508

 Score =  254 bits (648), Expect = 6e-72
 Identities = 150/478 (31%), Positives = 253/478 (52%), Gaps = 7/478 (1%)

Query: 7   LQAEHVAKAYAGVPALRDGRLSLRAGSVHALCGGNGAGKSTFLSILMGITQRDAGSILLN 66
           +Q   + K +    AL    L +  GS+HA+ G NGAGKST +++L G+ Q + G I L 
Sbjct: 9   VQMHGIVKMFGSFCALDHVDLDVERGSIHAILGENGAGKSTLMNVLYGLYQAEEGEIFLG 68

Query: 67  GAPVQFNRPSEALAAGIAMITQELEPIPYMTVAENIWLGREPRRAGCIVDNKALNRRTRE 126
           G  V  + P++A+A GI M+ Q    +   TV +NI LG+EP R   ++D KA  +R R+
Sbjct: 69  GEKVSISNPNDAIAHGIGMVHQHFMLVDNFTVTQNIILGQEPCRGAGLLDMKAAGQRVRD 128

Query: 127 LLDSLEFDVDATSPMHRLSVAQIQLVEIAKAFSHDCQVMIMDEPTSAIGEHEAQTLFKAI 186
           ++     +VD  + +  +SV   Q VEI KA      ++I+DEPT+ +   E   L + +
Sbjct: 129 IIAEYGLEVDPDAKIEDISVGMQQRVEILKALYRGADILILDEPTAVLTPQEIDRLVQIM 188

Query: 187 RRLTAQGAGIVYVSHRLSELAQIADDYSIFRDGAFVESGRMADIDRDHLVRGIVGQELTR 246
           R L A+G  I+ ++H+L E+   +D  +I R G +V +  +A +D + L   +VG  +  
Sbjct: 189 RGLAAKGKTIIIITHKLKEIKASSDTCTIIRRGKYVGAVDVAGVDEEELAEKMVGHHVNL 248

Query: 247 IDHKV----GRECAANTCLQVDNLSRAGEFHDISLQLRQGEILGIYGLMGSGRSEFLNCI 302
           +  K     G+E      L V +         ++L +  GEI+GI G+ G+G+ E +  I
Sbjct: 249 VVEKAPANPGKEVFRIDDLVVKDERGIEAVRHLNLSVCAGEIVGIAGIDGNGQKELVEAI 308

Query: 303 YGLTVADSGSVTLQGKPMPIGLPKATINAGMSLVTEDRKDSGLVLTGSILSNIALSAYK- 361
             LT A SG++T+ G+ +    P+  I+  +S + EDR+  GLVL  ++  N  L  Y+ 
Sbjct: 309 TCLTRAQSGTITVNGEEIQNTTPRNVIDHKVSTIHEDRQRRGLVLPFTVAENAVLEKYRT 368

Query: 362 -RLSSWSLINARKETQLAEDMVKRLQIKTTS-LELPVASMSGGNQQKVVLAKCLSTEPVC 419
                  +++  K  +  E ++    ++     +   A +SGGNQQKV++ + +S  P  
Sbjct: 369 PEFGEHGVLDRAKMHEFTEQLIHDYDVRPADCADHTAAGLSGGNQQKVIIGREISNNPDV 428

Query: 420 LLCDEPTRGIDEGAKQEIYHLLDQFVRGGGAAIVVSSEAPELLHLSDRIAVFKGGRLV 477
           L+  +PTRG+D GA + ++  L      G A +++S E  E++ ++D IAV   G +V
Sbjct: 429 LIAVQPTRGLDVGAIEYVHKTLVAERDRGTAILLISLELDEVMSVADTIAVIYAGAIV 486



 Score = 70.9 bits (172), Expect = 1e-16
 Identities = 59/235 (25%), Positives = 109/235 (46%), Gaps = 14/235 (5%)

Query: 12  VAKAYAGVPALRDGRLSLRAGSVHALCGGNGAGKSTFLSILMGITQRDAGSILLNGAPVQ 71
           V K   G+ A+R   LS+ AG +  + G +G G+   +  +  +T+  +G+I +NG  +Q
Sbjct: 268 VVKDERGIEAVRHLNLSVCAGEIVGIAGIDGNGQKELVEAITCLTRAQSGTITVNGEEIQ 327

Query: 72  FNRPSEALAAGIAMITQELEP----IPYMTVAENIWLGREPRRAGCIVDNKALNR-RTRE 126
              P   +   ++ I ++ +     +P+ TVAEN  L  E  R     ++  L+R +  E
Sbjct: 328 NTTPRNVIDHKVSTIHEDRQRRGLVLPF-TVAENAVL--EKYRTPEFGEHGVLDRAKMHE 384

Query: 127 LLDSLEFDVD------ATSPMHRLSVAQIQLVEIAKAFSHDCQVMIMDEPTSAIGEHEAQ 180
             + L  D D      A      LS    Q V I +  S++  V+I  +PT  +     +
Sbjct: 385 FTEQLIHDYDVRPADCADHTAAGLSGGNQQKVIIGREISNNPDVLIAVQPTRGLDVGAIE 444

Query: 181 TLFKAIRRLTAQGAGIVYVSHRLSELAQIADDYSIFRDGAFVESGRMADIDRDHL 235
            + K +     +G  I+ +S  L E+  +AD  ++   GA V++ +  D+D + +
Sbjct: 445 YVHKTLVAERDRGTAILLISLELDEVMSVADTIAVIYAGAIVDTFKQGDVDENRI 499



 Score = 65.1 bits (157), Expect = 6e-15
 Identities = 57/220 (25%), Positives = 100/220 (45%), Gaps = 13/220 (5%)

Query: 275 ISLQLRQGEILGIYGLMGSGRSEFLNCIYGLTVADSGSVTLQGKPMPIGLPKATINAGMS 334
           + L + +G I  I G  G+G+S  +N +YGL  A+ G + L G+ + I  P   I  G+ 
Sbjct: 27  VDLDVERGSIHAILGENGAGKSTLMNVLYGLYQAEEGEIFLGGEKVSISNPNDAIAHGIG 86

Query: 335 LVTEDRKDSGLVLTGSILSNIALSAYKRLSSWSLINARKETQLAEDMVKRLQIKTTSLEL 394
           +V +      LV   ++  NI L   +      L++ +   Q   D++    ++    + 
Sbjct: 87  MVHQHFM---LVDNFTVTQNIILGQ-EPCRGAGLLDMKAAGQRVRDIIAEYGLEVDP-DA 141

Query: 395 PVASMSGGNQQKVVLAKCLSTEPVCLLCDEPTRGIDEGAKQEIYHLLDQFVRG----GGA 450
            +  +S G QQ+V + K L      L+ DEPT  +     QEI  L+ Q +RG    G  
Sbjct: 142 KIEDISVGMQQRVEILKALYRGADILILDEPTAVL---TPQEIDRLV-QIMRGLAAKGKT 197

Query: 451 AIVVSSEAPELLHLSDRIAVFKGGRLVTISTDTALSQEAL 490
            I+++ +  E+   SD   + + G+ V       + +E L
Sbjct: 198 IIIITHKLKEIKASSDTCTIIRRGKYVGAVDVAGVDEEEL 237


Lambda     K      H
   0.319    0.135    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 547
Number of extensions: 24
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 3
Length of query: 495
Length of database: 508
Length adjustment: 34
Effective length of query: 461
Effective length of database: 474
Effective search space:   218514
Effective search space used:   218514
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources