GapMind for catabolism of small carbon sources

 

Protein WP_010537638.1 in Bacteroides faecis MAJ27

Annotation: NCBI__GCF_000226135.1:WP_010537638.1

Length: 523 amino acids

Source: GCF_000226135.1 in NCBI

Candidate for 10 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-arabinose catabolism BT0355 med Na+/glucose cotransporter (characterized, see rationale) 33% 92% 295.8 Sodium/myo-inositol cotransporter 2; Na(+)/myo-inositol cotransporter 2; Sodium-dependent glucose cotransporter; Sodium/glucose cotransporter KST1; rKST1; Sodium/myo-inositol transporter 2; SMIT2; Solute carrier family 5 member 11 31% 278.5
D-cellobiose catabolism SSS-glucose med Sodium/myo-inositol cotransporter 2; Na(+)/myo-inositol cotransporter 2; Sodium-dependent glucose cotransporter; Sodium/glucose cotransporter KST1; Sodium/myo-inositol transporter 2; SMIT2; Solute carrier family 5 member 11 (characterized) 30% 82% 274.2
D-glucose catabolism SSS-glucose med Sodium/myo-inositol cotransporter 2; Na(+)/myo-inositol cotransporter 2; Sodium-dependent glucose cotransporter; Sodium/glucose cotransporter KST1; Sodium/myo-inositol transporter 2; SMIT2; Solute carrier family 5 member 11 (characterized) 30% 82% 274.2
lactose catabolism SSS-glucose med Sodium/myo-inositol cotransporter 2; Na(+)/myo-inositol cotransporter 2; Sodium-dependent glucose cotransporter; Sodium/glucose cotransporter KST1; Sodium/myo-inositol transporter 2; SMIT2; Solute carrier family 5 member 11 (characterized) 30% 82% 274.2
D-maltose catabolism SSS-glucose med Sodium/myo-inositol cotransporter 2; Na(+)/myo-inositol cotransporter 2; Sodium-dependent glucose cotransporter; Sodium/glucose cotransporter KST1; Sodium/myo-inositol transporter 2; SMIT2; Solute carrier family 5 member 11 (characterized) 30% 82% 274.2
sucrose catabolism SSS-glucose med Sodium/myo-inositol cotransporter 2; Na(+)/myo-inositol cotransporter 2; Sodium-dependent glucose cotransporter; Sodium/glucose cotransporter KST1; Sodium/myo-inositol transporter 2; SMIT2; Solute carrier family 5 member 11 (characterized) 30% 82% 274.2
trehalose catabolism SSS-glucose med Sodium/myo-inositol cotransporter 2; Na(+)/myo-inositol cotransporter 2; Sodium-dependent glucose cotransporter; Sodium/glucose cotransporter KST1; Sodium/myo-inositol transporter 2; SMIT2; Solute carrier family 5 member 11 (characterized) 30% 82% 274.2
myo-inositol catabolism SMIT1 lo Sodium/myo-inositol cotransporter 2; Na(+)/myo-inositol cotransporter 2; Sodium-dependent glucose cotransporter; Sodium/glucose cotransporter KST1; rKST1; Sodium/myo-inositol transporter 2; SMIT2; Solute carrier family 5 member 11 (characterized) 31% 79% 278.5 Renal Na+:D-glucose symporter type 1 (Sglt1; Slc5a1) of 662 aas and 14 TMSs 32% 276.6
D-galactose catabolism SGLT1 lo sodium/glucose cotransporter 1 (characterized) 31% 80% 273.1 Sodium/myo-inositol cotransporter 2; Na(+)/myo-inositol cotransporter 2; Sodium-dependent glucose cotransporter; Sodium/glucose cotransporter KST1; rKST1; Sodium/myo-inositol transporter 2; SMIT2; Solute carrier family 5 member 11 31% 278.5
D-galactose catabolism sglS lo Sodium transporter (characterized, see rationale) 30% 100% 236.9 Sodium/myo-inositol cotransporter 2; Na(+)/myo-inositol cotransporter 2; Sodium-dependent glucose cotransporter; Sodium/glucose cotransporter KST1; rKST1; Sodium/myo-inositol transporter 2; SMIT2; Solute carrier family 5 member 11 31% 278.5

Sequence Analysis Tools

View WP_010537638.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MHVKFLDTLDWGILIAYFLILIAIGVWASSKRKKGSSLFLAERSLRWHHIGFSMWGTNVG
PSMLIASASAGFTTGIVSGNYAWYAFVFICLLAFVFAPRYLGTRVSTLPEFMGKRFGQST
RNILAWYTIVTILISWLALTLFAGGVLIRQVFDIPMWQSALILLIISAFFTMLGGLKAVA
YTNVYQMILLILVSATLAIVGICKVGGISALTDAVPADFWNLFRPNDDPAFPWLPIILGY
PVMGVWFWCTDQSMVQPVLAAKNLKEGQLGTNFTGWLKILDVPLYILPGIICLALFPQLE
NPDEAYMTMVTHLFPVGMVGLVLAVLTAALVSTIGSALNALSTVFTMDIYVKKIRPQARQ
KEIIRVGQIVTVVGALISVIITIAIDSIHGLNLFNVFQSVLGFIAPPMAAVFLFGVFWKR
TTTLAANAALTVGTVFSIGVGVLYLWVFPADQYAAWPHFMLLSFYLFVIIGIGMMVISLL
DKTPQAEILNMEKIEAKPARIVLVLWALLIVTMIGLYIFFNGH

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory