Finding step aatJ for L-asparagine catabolism in Rhodanobacter denitrificans 2APBS1
No candidates for aatJ: aspartate/asparagine ABC transporter, substrate-binding component AatJ
GapMind classifies a step as low confidence even if it does not find any candidates. You can still try to find candidates by using Curated BLAST (which searches the 6-frame translation) or by text search of the annotations (which may indicate weak homology, under 30% identity or 50% coverage, that GapMind does not consider). See the links below.
Definition of step aatJ
- Curated sequence Q88NY2: PP1071, component of Acidic amino acid uptake porter, AatJMQP
- Curated sequence AO353_16290: ABC transporter for L-aspartate, L-asparagine, L-glutamate, and L-glutamine, periplasmic substrate-binding component
- Curated sequence Pf1N1B4_771: ABC transporter for L-asparagine and L-glutamate, periplasmic substrate-binding component
- Curated sequence PfGW456L13_4770: ABC transporter for L-Asparagine and possibly other L-amino acids, periplasmic substrate-binding component
- Ignore hits to Q9I402 when looking for 'other' hits (Probable binding protein component of ABC transporter, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida)
- Curated sequence CH_002441: glutamate-aspartate periplasmic-binding protein. Glutamate/aspartate import solute-binding protein. YBEJ aka GltI aka B0655, component of Glutamate/aspartate porter. glutamate/aspartate ABC transporter periplasmic binding protein (EC 7.4.2.1). glutamate/aspartate ABC transporter periplasmic binding protein (EC 7.4.2.1)
- Comment: AatJMQP from Pseudomonas putida KT2440, TC 3.A.1.3.19, is quite similar to an aspartate ABC transporter from P. fluorescens N2E3 and to GltIJKL from E. coli. It is also very similar to asparagine/glutamate transporters from other Pseudomonas, which might transport aspartate as well. (aatJ = PP1071 or AO353_16290 or gltI)
Or cluster all characterized aatJ proteins
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory