Alignments for a candidate for adhBqn in Rhodanobacter denitrificans 2APBS1

GapMind for catabolism of small carbon sources

Alignments for a candidate for adhBqn in Rhodanobacter denitrificans 2APBS1

Align Alcohol dehydrogenase (quinone), cytochrome c subunit; ADH; Alcohol dehydrogenase (quinone), subunit II; Cytochrome c-553; Cytochrome c553; Ethanol:Q2 reductase; G3-ADH subunit II; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 (characterized)
to candidate WP_015448970.1 R2APBS1_RS17705 alcohol dehydrogenase

Query= SwissProt::P0A388
         (468 letters)



>NCBI__GCF_000230695.2:WP_015448970.1
          Length = 427

 Score =  260 bits (665), Expect = 5e-74
 Identities = 150/402 (37%), Positives = 214/402 (53%), Gaps = 26/402 (6%)

Query: 29  DSALVQKGAYVARLGDCVACHTALHGQSYAGGLEIKSPIGTIYSTNITPDPTYGIGRYTF 88
           D AL+ +G Y+A  GDC ACHTA  GQ YAGG  + +P G + + NITPDP  GIG+++F
Sbjct: 45  DPALIARGEYLATAGDCAACHTARDGQRYAGGRSLGTPFGDVPAPNITPDPDTGIGQWSF 104

Query: 89  AEFDEAVRHGIRKDGSTLYPAMPYPSFSRMTKEDMQALYAYFMHGVKPVAQPDKQPDISW 148
            +F  A+  G  + G  LYP   Y SF++++++D  A++AY +  + PV +PD    + +
Sbjct: 105 DDFWRALHEGKGRRGELLYPVFSYTSFTKVSRDDALAIFAY-LKSLPPVQRPDTALGLEF 163

Query: 149 PLSMRWPLGIWRMMFSPSPKDFTPAPGTDPEIARGDYLVTGPGHCGACHTPRGFAMQEKA 208
           P ++R  L  WR ++   P ++ P P    +  RG YLV G GHC  CH+ R        
Sbjct: 164 PYNVRSSLVAWRALYF-KPGEYRPDPTKSEQWNRGAYLVQGLGHCNECHSTR-------- 214

Query: 209 LDAAGGPD---FLSGG-APIDNWVAPSLRNDPVVGLGRWSEDDIYTFLKSGRIDHSAVFG 264
            D+ GG +    L+GG  P  +W AP L      GL  W   DI   LK+G+      FG
Sbjct: 215 -DSLGGIEQDRHLTGGQIPQLDWYAPDLSTQAGGGLDGWRAQDIVDLLKTGQSAKGTAFG 273

Query: 265 GMGDVVAWSTQYFTDDDLHAIAKYLKSLPPVPPSQGNYTYDPSTANMLASGNTASVPGAD 324
            M DVV  STQ+  D DL A+A YL+SLPP  P+        +    + + + A   G  
Sbjct: 274 PMADVVRLSTQHLRDADLQAVAVYLQSLPPRAPA--------AAVPFVQAASHAQ--GGK 323

Query: 325 TYVKECAICHRNDGGGVARMFPPLAGNPVVVTENPTSLVNVIAHGGVLPPSNWAPSAVAM 384
            Y + CA CH  DG GVA  +PPL GN  V      + +  +  GG  P +   P   +M
Sbjct: 324 LYAQHCADCHGKDGRGVAGTYPPLDGNTSVTEPTGINAIRSVLLGGFAPATAGNPQPYSM 383

Query: 385 PGYSKSLSAQQIADVVNFIRTSWGNKAPGTVTAADVTKLRDT 426
           P +++ L+ + +A VV +IR SW N+A   V  ADV   R+T
Sbjct: 384 PPFAQQLNDEDVAAVVGYIRQSWSNQA-AAVQPADVGTYRNT 424



 Score = 71.2 bits (173), Expect = 6e-17
 Identities = 88/304 (28%), Positives = 117/304 (38%), Gaps = 78/304 (25%)

Query: 154 WPLGIWRMMFSPSPKDFTPAPGTDPE-IARGDYLVTGPGHCGACHTPRGFAMQEKALDAA 212
           W LG  + + SP+ +    A   DP  IARG+YL T  G C ACHT R           A
Sbjct: 22  WYLGHGQPVASPAQRAADAAALRDPALIARGEYLATA-GDCAACHTAR------DGQRYA 74

Query: 213 GGPDFLSGGAPIDNWVAPSLRNDPVVGLGRWSEDDIYTFLKSGRIDHSAVFGGMGDVV-- 270
           GG    S G P  +  AP++  DP  G+G+WS DD +  L  G+       G  G+++  
Sbjct: 75  GG---RSLGTPFGDVPAPNITPDPDTGIGQWSFDDFWRALHEGK-------GRRGELLYP 124

Query: 271 AWSTQYFTD---DDLHAIAKYLKSLPPVPP-------------------------SQGNY 302
            +S   FT    DD  AI  YLKSLPPV                             G Y
Sbjct: 125 VFSYTSFTKVSRDDALAIFAYLKSLPPVQRPDTALGLEFPYNVRSSLVAWRALYFKPGEY 184

Query: 303 TYDPSTANMLASGNTASVPGADTYVKECAICH--RNDGGGVARMFPPLAGNPVVVTENPT 360
             DP+ +     G    V G    +  C  CH  R+  GG+               E   
Sbjct: 185 RPDPTKSEQWNRG-AYLVQG----LGHCNECHSTRDSLGGI---------------EQDR 224

Query: 361 SLVNVIAHGGVLPPSNW-APSAVAMPGYSKSLSAQQIADVVNFIRTSWGNKAPGTVTAAD 419
            L      GG +P  +W AP      G    L   +  D+V+ ++T    K       AD
Sbjct: 225 HLT-----GGQIPQLDWYAPDLSTQAG--GGLDGWRAQDIVDLLKTGQSAKGTAFGPMAD 277

Query: 420 VTKL 423
           V +L
Sbjct: 278 VVRL 281


Lambda     K      H
   0.317    0.134    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 779
Number of extensions: 51
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 468
Length of database: 427
Length adjustment: 33
Effective length of query: 435
Effective length of database: 394
Effective search space:   171390
Effective search space used:   171390
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources