Alignments for a candidate for glcD in Rhodanobacter denitrificans 2APBS1

GapMind for catabolism of small carbon sources

Alignments for a candidate for glcD in Rhodanobacter denitrificans 2APBS1

Align glycolate oxidase subunit glcD (characterized)
to candidate WP_015447114.1 R2APBS1_RS05170 FAD-binding oxidoreductase

Query= CharProtDB::CH_024646
         (499 letters)



>NCBI__GCF_000230695.2:WP_015447114.1
          Length = 463

 Score =  201 bits (511), Expect = 5e-56
 Identities = 148/460 (32%), Positives = 224/460 (48%), Gaps = 18/460 (3%)

Query: 22  LMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVP 81
           L  L   +P L +L T  E+  Y  D    +   PL + LP   E+V AI+   + LRV 
Sbjct: 6   LAELSRRLPALRLLATAAELEHYGRDWTRRWTPAPLAIALPAGAEEVQAIVRWANELRVA 65

Query: 82  VVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHN 141
           +V  G  TGLSGGA+     ++L + R   +LD N V R   VQPG+    +  A   H 
Sbjct: 66  IVPSGGRTGLSGGAVAANGELVLSLQRMNRLLDFNAVDRTLTVQPGMVLQHVHDAAREHG 125

Query: 142 LYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDAL-D 200
           L Y  D +++ +CSIGGN+A NAGG+  ++YG T   +  ++V   +GE L L    + +
Sbjct: 126 LLYPVDFAARGSCSIGGNIATNAGGIRVIRYGNTREWIAGLKVVAGNGELLDLNRGLIKN 185

Query: 201 SPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGII 260
           S G+D   L  GSEG LG+  E T+KL   PP ++V+L +   ++ A + V  +    ++
Sbjct: 186 SSGYDFRQLLIGSEGTLGIVVEATLKLTNPPPPSQVMLLALPDMD-ALMQVFALFRARLV 244

Query: 261 PGGLEMMDNLSIR-----AAEDFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLK 315
               E   + ++R      A+  I   YP      ++CE D  +   QE      +  L+
Sbjct: 245 LQAFEFFTDHALRHVLAHGAQRAIDGDYPY----YVVCEFDAADERQQEAALSAFERALE 300

Query: 316 AG-ATDVRLAQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRALPGVL-EGIARL 373
            G  +D  +AQ EA+    W  R+    ++    P  Y  D ++   A+P  L E  A L
Sbjct: 301 QGWVSDGVIAQSEAQAAALWRLREGITESLAPRRP--YKNDVSVRVSAVPAFLHEMQALL 358

Query: 374 SQQY-DLRVANVFHAGDGNMHPLILFDAN--EPGEFARAEELGGKILELCVEVGGSISGE 430
           +++Y    V    H GDGN+H  +L      E    A+ E +   +       GGSIS E
Sbjct: 359 AREYPQAEVVWFGHIGDGNLHINVLRPDGLAEDAFIAQCEHVTTLLAATLQRHGGSISAE 418

Query: 431 HGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGK 470
           HGIG  K   + +  ++ EI     V+  FDP+G+LNPGK
Sbjct: 419 HGIGLVKRAYLESTRSAAEIALMRGVRQVFDPNGILNPGK 458


Lambda     K      H
   0.320    0.140    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 556
Number of extensions: 29
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 499
Length of database: 463
Length adjustment: 34
Effective length of query: 465
Effective length of database: 429
Effective search space:   199485
Effective search space used:   199485
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources