Align MsmK aka SMU.882, component of The raffinose/stachyose transporter, MsmEFGK (MalK (3.A.1.1.27) can probably substitute for MsmK; Webb et al., 2008). This system may also transport melibiose, isomaltotriose and sucrose as well as isomaltosaccharides (characterized)
to candidate WP_015448118.1 R2APBS1_RS12060 ABC transporter ATP-binding protein
Query= TCDB::Q00752 (377 letters) >NCBI__GCF_000230695.2:WP_015448118.1 Length = 255 Score = 137 bits (346), Expect = 2e-37 Identities = 83/215 (38%), Positives = 126/215 (58%), Gaps = 13/215 (6%) Query: 37 VGPSGCGKSTTLRMVAGLEDITKGELKIDGEVV--NDKAPKDRDIAMVFQNYALYPHMSV 94 +G SG GKST LR++ GLE G +++DG + +D P R + V Q+ L+PH++ Sbjct: 43 IGSSGSGKSTVLRLLLGLEWPDHGHVEVDGRPLQRSDVLPLRRRVGYVIQDGGLFPHLTA 102 Query: 95 YDNMAFGLKLRHYSKEAIDKRVKEAAQILGL-TEFLERKPADLSGGQRQRVAMGRAIVRD 153 N+A + +++E I +R ++ A + L T LER PA+LSGGQRQRVA+ RA++ D Sbjct: 103 LGNLALLPRHLGWNRERIRQRAEQLAALTHLPTGVLERYPAELSGGQRQRVALMRALMAD 162 Query: 154 AKVFLMDEPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSSTK 213 L+DEPL LD +R ++ E+ +I ++G T I VTHD EA A+R+V++ Sbjct: 163 PDALLLDEPLGALDPVVRHELQDELKQIFDQLGKTVIVVTHDLAEAAWFAERLVLLRQ-- 220 Query: 214 NEDGSGTIGRVEQVGTPQELYNRPANKFVAGFIGS 248 G V Q GT ++L RPA+ FV F+ + Sbjct: 221 --------GAVLQDGTFRDLRERPADAFVNRFVAA 247 Lambda K H 0.318 0.135 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 187 Number of extensions: 6 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 377 Length of database: 255 Length adjustment: 27 Effective length of query: 350 Effective length of database: 228 Effective search space: 79800 Effective search space used: 79800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory