GapMind for catabolism of small carbon sources

 

Protein WP_009156897.1 in Saccharomonospora marina XMU15

Annotation: NCBI__GCF_000244955.1:WP_009156897.1

Length: 518 amino acids

Source: GCF_000244955.1 in NCBI

Candidate for 8 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
2-deoxy-D-ribonate catabolism aacS lo Acetoacetate--CoA ligase (EC 6.2.1.16) (characterized) 35% 93% 295.8 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 39% 345.5
2-deoxy-D-ribose catabolism aacS lo Acetoacetate--CoA ligase (EC 6.2.1.16) (characterized) 35% 93% 295.8 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 39% 345.5
L-leucine catabolism aacS lo Acetoacetate--CoA ligase (EC 6.2.1.16) (characterized) 35% 93% 295.8 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 39% 345.5
L-phenylalanine catabolism aacS lo Acetoacetate--CoA ligase (EC 6.2.1.16) (characterized) 35% 93% 295.8 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 39% 345.5
L-tyrosine catabolism aacS lo Acetoacetate--CoA ligase (EC 6.2.1.16) (characterized) 35% 93% 295.8 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 39% 345.5
4-hydroxybenzoate catabolism hcl lo 3-hydroxybenzoate--CoA/4-hydroxybenzoate--CoA ligase; 3-hydroxybenzoyl-CoA synthetase; EC 6.2.1.27; EC 6.2.1.37 (characterized) 31% 94% 225.3 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 39% 345.5
phenylacetate catabolism paaK lo medium-chain acyl-CoA ligase (EC 6.2.1.2); phenylacetate-CoA ligase (EC 6.2.1.30) (characterized) 30% 92% 211.1 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 39% 345.5
L-phenylalanine catabolism paaK lo medium-chain acyl-CoA ligase (EC 6.2.1.2); phenylacetate-CoA ligase (EC 6.2.1.30) (characterized) 30% 92% 211.1 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 39% 345.5

Sequence Analysis Tools

View WP_009156897.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MNRGIGAWVTKRAFLQGDYTAFVAGERTFTYADADRRTDQLASALAGLGVRRGDRVAALM
VNSVEFLETLLACAKLGAIMVPINVRLAAVEVGFILGDSGADVLVFSGQFTDIVRGALTE
PGVRVRHAVQVGEGGYERGPSYDELLASASPDFAAADVAGTDTAMLMYTSGTTGRPKGAM
LTHDNLLWNAINVLGTEHPLSSRDVTVTVAPMFHIGGLGVHSLPLLYVGGTNVILPNFDP
VGTLTAMAEHRATVQFMVPAMWVAITQVPNFGSYDLSSLELAMGGGAPCPLPMIEFLNER
GVPFIEGFGMTETAPLVSILNSAHARSKAGSIGRVAMHVEARIVDDDDRDVPTNTVGELV
LRGPNIFAGYWMRPEATAEAFRNGWFHTGDLGRMDEEGYITLVDRKKDMIISGGENVYPI
EVEQVLFKHPKVADAAVVGGPDPRWGERVVAVVVPDPGAGPDPGSEELVTWCRERLAHFK
CPREVHFVDALPRNATGKLLKTVLREKYAGVTDGVVVR

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory