Alignments for a candidate for acs in Saccharomonospora marina XMU15

GapMind for catabolism of small carbon sources

Alignments for a candidate for acs in Saccharomonospora marina XMU15

Align Acetate/butyrate--CoA ligase AAE7, peroxisomal; AMP-binding protein 7; AtAMPBP7; Acetyl-CoA synthetase; Acyl-activating enzyme 7; Butyryl-CoA synthetase; Protein ACETATE NON-UTILIZING 1; EC 6.2.1.1; EC 6.2.1.2 (characterized)
to candidate WP_009152150.1 SACMADRAFT_RS02230 AMP-binding protein

Query= SwissProt::Q8VZF1
         (569 letters)



>NCBI__GCF_000244955.1:WP_009152150.1
          Length = 525

 Score =  456 bits (1172), Expect = e-132
 Identities = 242/542 (44%), Positives = 337/542 (62%), Gaps = 39/542 (7%)

Query: 18  NYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALADRSIGPGSTVAI 77
           ++  LTP+ +LDRAA  H +R +V+ G   +T+ Q +DRCRRLA  LA  +   G  VA+
Sbjct: 6   SFEPLTPVSYLDRAAAAHGSRTAVVDGEHRWTYTQLHDRCRRLAGGLA--APAEGRPVAV 63

Query: 78  IAPNIPAMYEAHFGVPMCGAVLNCVNIRLNAPTVAFLLSHSQSSVIMVDQEFFTLAEDSL 137
           +A N   + EA+FGVP  G  L  VN RL+A  VA++L H  + V++ D  F    +D  
Sbjct: 64  LAANTHVLLEANFGVPWAGVPLVAVNTRLSAREVAYILEHCGARVLVHDPAF----DD-- 117

Query: 138 RLMEEKAGSSFKRPLLIVIGDHTCAPESLNRALSKGAIEYEDFLATGDPNYPWQPPADEW 197
            L+ +  G   + P++I  G+                 EYE  LA  +P    + PADE 
Sbjct: 118 -LVAQARGELAEPPVVIRAGE-----------------EYEQLLAGAEPMA--RTPADER 157

Query: 198 QSIALGYTSGTTASPKGVVLHHRGAYIMALSNPLIWGMQDGAVYLWTLPMFHCNGWCFPW 257
             +++ YTSGTT  PKGV+ HHRGAY+ AL+     G+   AV+LWTLPMFHCNGWCFPW
Sbjct: 158 ALLSINYTSGTTGRPKGVMYHHRGAYLQALAMVAHTGLSPSAVHLWTLPMFHCNGWCFPW 217

Query: 258 SLAVLSGTSICLRQVTAKEVYSMIAKYKVTHFCAAPVVLNAIVNAPKEDTILPLPHTVHV 317
           ++   + T +CL +V   EV+ ++ +  VTH   AP VL+ +  A   +   PL  TV V
Sbjct: 218 AVTAAAATHVCLPKVEPAEVWRLLRQEGVTHLEGAPTVLSMLAYA---EQAAPLERTVRV 274

Query: 318 MTAGAAPPPSVLFSMNQKGFRVAHTYGLSETYGPSTVCAWKPEWDSLPPETQAKLNARQG 377
            T GA P P++L  M + GF V H YGL+ET+GP+ VC W+PEW++L  E QA+L ARQG
Sbjct: 275 ATGGAPPTPAILRRMGELGFDVTHLYGLTETFGPAMVCDWRPEWNALAAEEQARLKARQG 334

Query: 378 VRYTGMEQLDVIDTQTGKPVPADGKTAGEIVFRGNMVMKGYLKNPEANKETFAGGWFHSG 437
           V      Q+ V+    G  VPADG++ G+I  RGN VM GY ++ EA ++    GWF +G
Sbjct: 335 VGNMISCQVRVV-ADDGTDVPADGESVGQIALRGNNVMLGYFRDEEATRQAAPDGWFRTG 393

Query: 438 DIAVKHPDNYIEIKDRSKDVIISGGENISSVEVENVVYHHPAVLEASVVARPDERWQESP 497
           D+ V+HPD Y+E++DRSKDVIISGGENI+SVEVE  +  HPAV E +V+A PD+ W E P
Sbjct: 394 DLGVRHPDGYVELRDRSKDVIISGGENIASVEVEQAIAEHPAVFEVAVIAVPDDHWGEVP 453

Query: 498 CAFVTLKSDYEKHDQNKLAQ-DIMKFCREKLPAYWVPKSVVFGPLPKTATGKIQKHILRT 556
            A+VT+      H+   +++ +++   RE+L  +  PKSVVFG LPKT+TGKIQK++LR 
Sbjct: 454 AAYVTV------HEGASVSESELIAHVRERLAHFKAPKSVVFGELPKTSTGKIQKYVLRE 507

Query: 557 KA 558
           +A
Sbjct: 508 RA 509


Lambda     K      H
   0.319    0.134    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 849
Number of extensions: 32
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 569
Length of database: 525
Length adjustment: 36
Effective length of query: 533
Effective length of database: 489
Effective search space:   260637
Effective search space used:   260637
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources