GapMind for catabolism of small carbon sources

 

L-fucose catabolism in Megamonas funiformis YIT 11815

Best path

fucP, fucU, fucI, fucK, fucA, tpi, fucO

Rules

Overview: Fucose degradation in GapMind is based on the MetaCyc pathway via L-fuculose (link) or the oxidative pathway via 2,4-diketo-3-deoxy-L-fuconate (KDF) hydrolase (PMC6336799).

23 steps (12 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
fucP L-fucose:H+ symporter FucP HMPREF9454_RS03085 HMPREF9454_RS06625
fucU L-fucose mutarotase FucU HMPREF9454_RS03080
fucI L-fucose isomerase FucI HMPREF9454_RS12525
fucK L-fuculose kinase FucK HMPREF9454_RS03075
fucA L-fuculose-phosphate aldolase FucA HMPREF9454_RS12520 HMPREF9454_RS08755
tpi triose-phosphate isomerase HMPREF9454_RS03395 HMPREF9454_RS03400
fucO L-lactaldehyde reductase HMPREF9454_RS08145 HMPREF9454_RS08515
Alternative steps:
aldA lactaldehyde dehydrogenase
BPHYT_RS34240 ABC transporter for L-fucose, permease component
BPHYT_RS34245 ABC transporter for L-fucose, ATPase component
BPHYT_RS34250 ABC transporter for L-fucose, substrate-binding component
fdh L-fucose 1-dehydrogenase HMPREF9454_RS06485
fucD L-fuconate dehydratase HMPREF9454_RS07630
fucDH 2-keto-3-deoxy-L-fuconate 4-dehydrogenase HMPREF9454_RS06290 HMPREF9454_RS06485
fuconolactonase L-fucono-1,5-lactonase
HSERO_RS05250 ABC transporter for L-fucose, ATPase component
HSERO_RS05255 ABC transporter for L-fucose, permease component
HSERO_RS05260 ABC transporter for L-fucose, substrate-binding component
KDF-hydrolase 2,4-diketo-3-deoxy-L-fuconate hydrolase HMPREF9454_RS01860
SM_b21103 ABC transporter for L-fucose, substrate-binding component
SM_b21104 ABC transporter for L-fucose, permease component 1
SM_b21105 ABC transporter for L-fucose, permease component 2
SM_b21106 ABC transporter for L-fucose, ATPase component HMPREF9454_RS05390 HMPREF9454_RS01120

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory