Alignments for a candidate for ptsG-crr in Megamonas funiformis YIT 11815

GapMind for catabolism of small carbon sources

Alignments for a candidate for ptsG-crr in Megamonas funiformis YIT 11815

Align PTS system glucose-specific EIICBA component; EIICBA-Glc; EII-Glc; EIICBA-Glc 1; EC 2.7.1.199 (characterized)
to candidate WP_008537413.1 HMPREF9454_RS01155 PTS transporter subunit EIIC

Query= SwissProt::Q57071
         (675 letters)



>NCBI__GCF_000245775.1:WP_008537413.1
          Length = 691

 Score =  340 bits (872), Expect = 1e-97
 Identities = 230/683 (33%), Positives = 355/683 (51%), Gaps = 62/683 (9%)

Query: 8   QLQRIGKALMLPVAILPAAGLLLALGNAFQGDALQSLMPFIKAEGFQNVAKMMEGAGGII 67
           ++QR G A+  PV +    G+ +A+    +   +   +   K  G+ +   ++E     +
Sbjct: 11  KIQRFGGAMFTPVIMFGVFGIFVAVSILCKNPMILGSIAE-KGTGWYDFWYIVEQGAWTV 69

Query: 68  FDNLAIIFALGVAIGLASGDGVAAIA-AFVGFIVLN-------KTMGMFLGVTPEKAADA 119
           F+ L ++F +G+ IGLA  +   A+  AF+ +++ N          G   GV   + A  
Sbjct: 70  FNQLPLLFVIGLPIGLAKKENARAVMEAFLIYLIFNYFISAMLNLWGASFGVDFAQEAVP 129

Query: 120 ATGFANVLGIPTLQTGVFGGIIIGALAAWCYNKFYNISLPSYLGFFAGKRFVPIMMATCS 179
            +G   +  I TL T + G I+I ++A W +NK +++ +P +LG F G   V I      
Sbjct: 130 GSGLTTIANIKTLDTSMVGAILISSIAVWLHNKLFDVDVPEWLGLFKGSSLVVI----AG 185

Query: 180 FILAFPMAI----IWPSIQGGLNAFSEGLLASNTGLAVFLFGFIKRLLIPFGLHHIFHAP 235
           F+L  P+A+    IWP  Q  L A  +G L     L V+++  ++R+LIP GLHH  + P
Sbjct: 186 FVLMLPIALLFCLIWPHFQS-LIASLQGFLTGAGVLGVWVYTSLERILIPTGLHHFIYIP 244

Query: 236 FWFEFGSYKNAAGQIIHGDQRIFIEQIRDNVPLTAGK---FMQGEFPV----MMFGLPAA 288
           F +           +  G Q  +++ I++    T      F QG F +     MFG P  
Sbjct: 245 FIY-------GPAVVDGGIQAYWLQHIQEFAQSTKPLIELFPQGGFALHGMSKMFGCPGI 297

Query: 289 ALAIYQTAKKENKKVVAGLMLSGALTSFLTGITEPLEFSFLFVAPLLFFIHAVLDGLSFL 348
           ALAIY +AKKEN+K V  L+   A+ + L GITEPLEF+FLFVAP+LF IHA+L G    
Sbjct: 298 ALAIYFSAKKENRKRVGSLLFPAAIVAVLCGITEPLEFTFLFVAPVLFLIHAILAGTLAA 357

Query: 349 ILYLLDLHLGYTFSGGFIDFFL----LGILPNKTQWWLV-IPVGLVYAAIYYIIFRFLIV 403
            +Y     +   F GG +D  L    + +  N    +L  I +GL++  I++++FRFLIV
Sbjct: 358 TMYFFG--VTGNFGGGLLDTDLFQNWIPLFNNHWDTYLTQIIIGLIFTCIWFVVFRFLIV 415

Query: 404 KFNFKTPGR-EDKEVK-------SSNVAASELPFKV---LDAMGGKANIKHLDACITRLR 452
           K N KTPGR +D+E+K          VA  E   K    L+A+GGK NI  +  C TRLR
Sbjct: 416 KLNLKTPGRGDDEEIKLYRKADYKQKVAGDERDRKAAAFLEALGGKNNIVDVTNCATRLR 475

Query: 453 VEVNDKAKV-DVQELKDLGASGVLEVGNNMQAIFGPKSDQIKHDMQQIMDGKITSPEETT 511
           V V D + V D       GA G++  G  +Q I G    Q++   + ++ G     +E  
Sbjct: 476 VTVKDDSLVKDTNVFMAAGAHGLVHNGCAIQVIVGLSVPQVRERFEALLQGPDVLQKEEL 535

Query: 512 VTEEGDKETAEIAAAGGGVVYAPIKGEVVDISEVPDKVFSEKMMGDGIAIKPETGEVVAP 571
           V+   DK  +  A A          G++V I  VPD+ F+ + MGDG+AI P    + AP
Sbjct: 536 VSNT-DKSPSLKAFA---------TGDLVSIENVPDEAFASRAMGDGVAIIPTEKYITAP 585

Query: 572 FDGVVKMVF-PTKHAIGLESKDGIELLIHFGLETVKLEGKGFDILVKENDNIVLGQPLMK 630
            +G V MV   T HA+GL+    +E+LIH G++TV ++G GF +LVK  D + +G  L++
Sbjct: 586 ANGSVMMVMEETGHALGLKLDTDMEILIHIGIDTVNMKGDGFKVLVKAGDEVKVGDKLVE 645

Query: 631 VDLDYIKEHADSTITPIVVTNLN 653
           +D++ IK    + IT ++ TN +
Sbjct: 646 IDIEKIKRAGYNPITIMIATNFD 668


Lambda     K      H
   0.324    0.143    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1118
Number of extensions: 67
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 675
Length of database: 691
Length adjustment: 39
Effective length of query: 636
Effective length of database: 652
Effective search space:   414672
Effective search space used:   414672
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources