Alignments for a candidate for acs in Sporolactobacillus vineae SL153

GapMind for catabolism of small carbon sources

Alignments for a candidate for acs in Sporolactobacillus vineae SL153

Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate WP_010631183.1 RH97_RS04695 AMP-binding protein

Query= SwissProt::P39062
         (572 letters)



>NCBI__GCF_000246965.1:WP_010631183.1
          Length = 532

 Score =  288 bits (738), Expect = 3e-82
 Identities = 177/516 (34%), Positives = 282/516 (54%), Gaps = 22/516 (4%)

Query: 54  ESFRKNKVALYYKDAKRDEK-YTFKEMKEESNRAGNVLRRYGNVEKGDRVFIFMPRSPEL 112
           + F   ++AL ++  K ++K  T+ ++ +   +  NVL   G ++KGDR+   +PR  + 
Sbjct: 19  DRFDSERIALRWESDKGEKKTITYHQLIQRGKQFANVLSGLG-LKKGDRIMTIVPRIIDA 77

Query: 113 YFIMLGAIKIGAIAGPLFEAFMEGAVKDRLENSEAKVVVTTPELLERIPVDK--LPHLQH 170
           Y I LGA++ G    P  E      +  RL   EAK +V   +   ++   K  LP L++
Sbjct: 78  YVIYLGALRAGIAVIPGSEMLRSADIALRLSQGEAKAIVAYRDYTSQVDGIKETLPALKY 137

Query: 171 VFVVGGEAESGTNIINYDEAAKQESTRLDIEWMDKKDGFLLHYTSGSTGTPKGVLHVHEA 230
              V GE +   ++      A  + T +      K D   L YTSG++GTPK V+H H  
Sbjct: 138 KISVDGETDGWLSMNRLVSEASADFTTVRTS---KDDMAFLTYTSGTSGTPKAVIHSHGW 194

Query: 231 MIQQYQTG--KWVLDLKEEDIYWCTADPGWVTGTVYGIFAPWLN----GATNVIVGGRFS 284
                +    +W LD+ + D +W TA PGW       I++P+L+    G T ++  GRF 
Sbjct: 195 AYAHLRIAPSRW-LDIHDGDTFWATAGPGWQKW----IWSPFLSVLGKGVTGLVYFGRFH 249

Query: 285 PESWYGTIEQLGVNVWYSAPTAFRMLMGAGDEMAAKYDLTSLRHVLSVGEPLNPEVIRWG 344
           PE     ++   VNV    PT +R++    +    KYDL+ LR+  S GE LN EV    
Sbjct: 250 PEKQLQLLQDYRVNVLCCTPTEYRLMAKVNN--LGKYDLSHLRNACSAGEALNREVTDIF 307

Query: 345 HKVFNKRIHDTWWMTETGSQLICNYPCMDIKPGSMGKPIPGVEAAIVDNQGNELPPYRMG 404
            + F+ ++ D +  TE+ + LI       I+ GSMGK I      ++D +G+ +P   +G
Sbjct: 308 KRTFDIQLKDGYGQTES-TLLIGTMVGDQIRVGSMGKAILDPFITVIDEEGHPVPDGTVG 366

Query: 405 NLAIKKGWPSMMHTIWNNPEKYESYFMPGGWYVSGDSAYMDEEGYFWFQGRVDDVIMTSG 464
           N+A+++ +P++    ++  ++YE+  + G ++++GD A  D++GYFWF GR DDVI++SG
Sbjct: 367 NIAVRRDFPALFRGYFHQEDRYEAA-VRGNYFLTGDRAAKDKDGYFWFHGRRDDVIISSG 425

Query: 465 ERVGPFEVESKLVEHPAIAEAGVIGKPDPVRGEIIKAFIALREGFEPSDKLKEEIRLFVK 524
             +GP EVE  L++  A+ +  V+G P  +RG I+KAF+ LR+G+EPS  L  E++ FVK
Sbjct: 426 YTIGPVEVEDALLKSQAVKDCAVVGIPHEIRGNIVKAFVILRDGYEPSQDLIHELQTFVK 485

Query: 525 QGLAAHAAPREIEFKDKLPKTRSGKIMRRVLKAWEL 560
           +  A +  PREIEF   LPKT SGKI R  L+  E+
Sbjct: 486 KETAPYKYPREIEFVQDLPKTISGKIKRSALRDLEV 521


Lambda     K      H
   0.318    0.136    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 793
Number of extensions: 45
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 572
Length of database: 532
Length adjustment: 36
Effective length of query: 536
Effective length of database: 496
Effective search space:   265856
Effective search space used:   265856
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources