Alignments for a candidate for acs in Sporolactobacillus vineae SL153

GapMind for catabolism of small carbon sources

Alignments for a candidate for acs in Sporolactobacillus vineae SL153

Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate WP_010631218.1 RH97_RS04870 acetate--CoA ligase

Query= SwissProt::P39062
         (572 letters)



>NCBI__GCF_000246965.1:WP_010631218.1
          Length = 573

 Score =  865 bits (2235), Expect = 0.0
 Identities = 407/567 (71%), Positives = 467/567 (82%)

Query: 6   LPAIEGDHNLKNYEETYRHFDWAEAEKHFSWHETGKLNAAYEAIDRHAESFRKNKVALYY 65
           LPA  G H+L N E+  + F W +AE  FSW +TGK+N AYEAIDRHAE  R  + ALYY
Sbjct: 7   LPAEHGTHDLDNIEKLRKSFQWKQAENFFSWKKTGKMNLAYEAIDRHAEHGRGEQTALYY 66

Query: 66  KDAKRDEKYTFKEMKEESNRAGNVLRRYGNVEKGDRVFIFMPRSPELYFIMLGAIKIGAI 125
            D  RDE YTF ++K  +N+A N+L+    VEKGDRVFIFMPRSPELY + LG IK GAI
Sbjct: 67  SDQVRDETYTFNQLKRLTNQAANILKNDAGVEKGDRVFIFMPRSPELYILFLGVIKAGAI 126

Query: 126 AGPLFEAFMEGAVKDRLENSEAKVVVTTPELLERIPVDKLPHLQHVFVVGGEAESGTNII 185
            GPLFEAFMEGAV+DRLE+S A V+ TTPELL R+P  +LP+L+ + +VG   +     I
Sbjct: 127 VGPLFEAFMEGAVRDRLEDSGASVLCTTPELLSRVPYTELPNLKKIILVGDTVQDDGYFI 186

Query: 186 NYDEAAKQESTRLDIEWMDKKDGFLLHYTSGSTGTPKGVLHVHEAMIQQYQTGKWVLDLK 245
           +Y +A  +      IEWMDK DG +LHYTSGSTG PKGVLHVH AM+Q  QTG+WVLDLK
Sbjct: 187 DYQKAMDRAEPTFPIEWMDKDDGLILHYTSGSTGKPKGVLHVHYAMVQHAQTGRWVLDLK 246

Query: 246 EEDIYWCTADPGWVTGTVYGIFAPWLNGATNVIVGGRFSPESWYGTIEQLGVNVWYSAPT 305
           ++DIYWCTADPGWVTGT YGIFAPWLNGA NV+ GGRFSP+ WY TIE+  V VWYSAPT
Sbjct: 247 KDDIYWCTADPGWVTGTSYGIFAPWLNGAANVVRGGRFSPQDWYETIEKYQVTVWYSAPT 306

Query: 306 AFRMLMGAGDEMAAKYDLTSLRHVLSVGEPLNPEVIRWGHKVFNKRIHDTWWMTETGSQL 365
           AFRMLMGAG  +A ++DL+SLRHVLSVGEPLNPEV++WG +V++KRIHDTWWMTETG+QL
Sbjct: 307 AFRMLMGAGKALADQHDLSSLRHVLSVGEPLNPEVVKWGWEVYHKRIHDTWWMTETGAQL 366

Query: 366 ICNYPCMDIKPGSMGKPIPGVEAAIVDNQGNELPPYRMGNLAIKKGWPSMMHTIWNNPEK 425
           ICN P M IK GSMGKP+PG+ AAIVDN G+ELPP  MGNLAIKKGWPSMMHTIWNNPEK
Sbjct: 367 ICNVPSMTIKAGSMGKPLPGITAAIVDNSGHELPPNTMGNLAIKKGWPSMMHTIWNNPEK 426

Query: 426 YESYFMPGGWYVSGDSAYMDEEGYFWFQGRVDDVIMTSGERVGPFEVESKLVEHPAIAEA 485
           + SYF P GWYVSGDSAYMDE+GYFWFQGRVDDVIMTSGER+GPFEVESKLVEHPA++EA
Sbjct: 427 FNSYFTPNGWYVSGDSAYMDEDGYFWFQGRVDDVIMTSGERIGPFEVESKLVEHPAVSEA 486

Query: 486 GVIGKPDPVRGEIIKAFIALREGFEPSDKLKEEIRLFVKQGLAAHAAPREIEFKDKLPKT 545
           GVIG PDPVRGEIIKAFI+LREGF  ++ LK EI  FVK GLAAHAAP++I+F +KLPKT
Sbjct: 487 GVIGIPDPVRGEIIKAFISLREGFTATESLKYEIHHFVKNGLAAHAAPKKIDFLEKLPKT 546

Query: 546 RSGKIMRRVLKAWELNLPAGDLSTMED 572
           RSGKIMRRVLKAWELNLP GDLSTMED
Sbjct: 547 RSGKIMRRVLKAWELNLPTGDLSTMED 573


Lambda     K      H
   0.318    0.136    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1179
Number of extensions: 41
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 572
Length of database: 573
Length adjustment: 36
Effective length of query: 536
Effective length of database: 537
Effective search space:   287832
Effective search space used:   287832
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources